Ectomycorrhizal Fungal Strains Facilitate Cd<sup>2+</sup> Enrichment in a Woody Hyperaccumulator under Co-Existing Stress of Cadmium and Salt
Cadmium (Cd<sup>2+</sup>) pollution occurring in salt-affected soils has become an increasing environmental concern in the world. Fast-growing poplars have been widely utilized for phytoremediation of soil contaminating heavy metals (HMs). However, the woody Cd<sup>2+</sup>-h...
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oai:doaj.org-article:a0dfe0adf85347f9a2c21350ed2cdf642021-11-11T17:07:27ZEctomycorrhizal Fungal Strains Facilitate Cd<sup>2+</sup> Enrichment in a Woody Hyperaccumulator under Co-Existing Stress of Cadmium and Salt10.3390/ijms2221116511422-00671661-6596https://doaj.org/article/a0dfe0adf85347f9a2c21350ed2cdf642021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11651https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Cadmium (Cd<sup>2+</sup>) pollution occurring in salt-affected soils has become an increasing environmental concern in the world. Fast-growing poplars have been widely utilized for phytoremediation of soil contaminating heavy metals (HMs). However, the woody Cd<sup>2+</sup>-hyperaccumulator, <i>Populus</i> × <i>canescens</i>, is relatively salt-sensitive and therefore cannot be directly used to remediate HMs from salt-affected soils. The aim of the present study was to testify whether colonization of <i>P.</i> × <i>canescens</i> with ectomycorrhizal (EM) fungi, a strategy known to enhance salt tolerance, provides an opportunity for affordable remediation of Cd<sup>2+</sup>-polluted saline soils. Ectomycorrhization with <i>Paxillus involutus</i> strains facilitated Cd<sup>2+</sup> enrichment in <i>P.</i> × <i>canescens</i> upon CdCl<sub>2</sub> exposures (50 μM, 30 min to 24 h). The fungus-stimulated Cd<sup>2+</sup> in roots was significantly restricted by inhibitors of plasmalemma H<sup>+</sup>-ATPases and Ca<sup>2+</sup>-permeable channels (CaPCs), but stimulated by an activator of plasmalemma H<sup>+</sup>-ATPases. NaCl (100 mM) lowered the transient and steady-state Cd<sup>2+</sup> influx in roots and fungal mycelia. Noteworthy, <i>P. involutus</i> colonization partly reverted the salt suppression of Cd<sup>2+</sup> uptake in poplar roots. EM fungus colonization upregulated transcription of plasmalemma H<sup>+</sup>-ATPases (<i>PcHA4</i>, <i>8</i>, <i>11</i>) and annexins (<i>Pc</i><i>ANN1</i>, <i>2</i>, <i>4</i>), which might mediate Cd<sup>2+</sup> conductance through CaPCs. EM roots retained relatively highly expressed <i>PcHAs</i> and <i>PcANNs</i>, thus facilitating Cd<sup>2+</sup> enrichment under co-occurring stress of cadmium and salinity. We conclude that ectomycorrhization of woody hyperaccumulator species such as poplar could improve phytoremediation of Cd<sup>2+</sup> in salt-affected areas.Chen DengZhimei ZhuJian LiuYing ZhangYinan ZhangDade YuSiyuan HouYanli ZhangJun YaoHuilong ZhangNan ZhaoGang SaYuhong ZhangXujun MaRui ZhaoAndrea PolleShaoliang ChenMDPI AGarticleannexinscalcium-permeable channelsCd fluxMAJNaClNAUBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11651, p 11651 (2021) |
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| language |
EN |
| topic |
annexins calcium-permeable channels Cd flux MAJ NaCl NAU Biology (General) QH301-705.5 Chemistry QD1-999 |
| spellingShingle |
annexins calcium-permeable channels Cd flux MAJ NaCl NAU Biology (General) QH301-705.5 Chemistry QD1-999 Chen Deng Zhimei Zhu Jian Liu Ying Zhang Yinan Zhang Dade Yu Siyuan Hou Yanli Zhang Jun Yao Huilong Zhang Nan Zhao Gang Sa Yuhong Zhang Xujun Ma Rui Zhao Andrea Polle Shaoliang Chen Ectomycorrhizal Fungal Strains Facilitate Cd<sup>2+</sup> Enrichment in a Woody Hyperaccumulator under Co-Existing Stress of Cadmium and Salt |
| description |
Cadmium (Cd<sup>2+</sup>) pollution occurring in salt-affected soils has become an increasing environmental concern in the world. Fast-growing poplars have been widely utilized for phytoremediation of soil contaminating heavy metals (HMs). However, the woody Cd<sup>2+</sup>-hyperaccumulator, <i>Populus</i> × <i>canescens</i>, is relatively salt-sensitive and therefore cannot be directly used to remediate HMs from salt-affected soils. The aim of the present study was to testify whether colonization of <i>P.</i> × <i>canescens</i> with ectomycorrhizal (EM) fungi, a strategy known to enhance salt tolerance, provides an opportunity for affordable remediation of Cd<sup>2+</sup>-polluted saline soils. Ectomycorrhization with <i>Paxillus involutus</i> strains facilitated Cd<sup>2+</sup> enrichment in <i>P.</i> × <i>canescens</i> upon CdCl<sub>2</sub> exposures (50 μM, 30 min to 24 h). The fungus-stimulated Cd<sup>2+</sup> in roots was significantly restricted by inhibitors of plasmalemma H<sup>+</sup>-ATPases and Ca<sup>2+</sup>-permeable channels (CaPCs), but stimulated by an activator of plasmalemma H<sup>+</sup>-ATPases. NaCl (100 mM) lowered the transient and steady-state Cd<sup>2+</sup> influx in roots and fungal mycelia. Noteworthy, <i>P. involutus</i> colonization partly reverted the salt suppression of Cd<sup>2+</sup> uptake in poplar roots. EM fungus colonization upregulated transcription of plasmalemma H<sup>+</sup>-ATPases (<i>PcHA4</i>, <i>8</i>, <i>11</i>) and annexins (<i>Pc</i><i>ANN1</i>, <i>2</i>, <i>4</i>), which might mediate Cd<sup>2+</sup> conductance through CaPCs. EM roots retained relatively highly expressed <i>PcHAs</i> and <i>PcANNs</i>, thus facilitating Cd<sup>2+</sup> enrichment under co-occurring stress of cadmium and salinity. We conclude that ectomycorrhization of woody hyperaccumulator species such as poplar could improve phytoremediation of Cd<sup>2+</sup> in salt-affected areas. |
| format |
article |
| author |
Chen Deng Zhimei Zhu Jian Liu Ying Zhang Yinan Zhang Dade Yu Siyuan Hou Yanli Zhang Jun Yao Huilong Zhang Nan Zhao Gang Sa Yuhong Zhang Xujun Ma Rui Zhao Andrea Polle Shaoliang Chen |
| author_facet |
Chen Deng Zhimei Zhu Jian Liu Ying Zhang Yinan Zhang Dade Yu Siyuan Hou Yanli Zhang Jun Yao Huilong Zhang Nan Zhao Gang Sa Yuhong Zhang Xujun Ma Rui Zhao Andrea Polle Shaoliang Chen |
| author_sort |
Chen Deng |
| title |
Ectomycorrhizal Fungal Strains Facilitate Cd<sup>2+</sup> Enrichment in a Woody Hyperaccumulator under Co-Existing Stress of Cadmium and Salt |
| title_short |
Ectomycorrhizal Fungal Strains Facilitate Cd<sup>2+</sup> Enrichment in a Woody Hyperaccumulator under Co-Existing Stress of Cadmium and Salt |
| title_full |
Ectomycorrhizal Fungal Strains Facilitate Cd<sup>2+</sup> Enrichment in a Woody Hyperaccumulator under Co-Existing Stress of Cadmium and Salt |
| title_fullStr |
Ectomycorrhizal Fungal Strains Facilitate Cd<sup>2+</sup> Enrichment in a Woody Hyperaccumulator under Co-Existing Stress of Cadmium and Salt |
| title_full_unstemmed |
Ectomycorrhizal Fungal Strains Facilitate Cd<sup>2+</sup> Enrichment in a Woody Hyperaccumulator under Co-Existing Stress of Cadmium and Salt |
| title_sort |
ectomycorrhizal fungal strains facilitate cd<sup>2+</sup> enrichment in a woody hyperaccumulator under co-existing stress of cadmium and salt |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/a0dfe0adf85347f9a2c21350ed2cdf64 |
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