Mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells

Abstract White mineral trioxide aggregate (WMTA) is a root canal treatment material, which is known to exhibit a dark brown color when in contact with sodium hypochlorite solution (NaOCl). This study aimed to investigate the effects of NaOCl on the surface properties of WMTA discs and WMTA-induced o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kozue Yamashita, Atsushi Tomokiyo, Taiga Ono, Keita Ipposhi, M. Anas Alhasan, Akira Tsuchiya, Sayuri Hamano, Hideki Sugii, Shinichiro Yoshida, Tomohiro Itoyama, Hidefumi Maeda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a0e6c86099b0426bb5746dc258c2ee62
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a0e6c86099b0426bb5746dc258c2ee62
record_format	dspace
spelling	oai:doaj.org-article:a0e6c86099b0426bb5746dc258c2ee622021-11-14T12:23:27ZMineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells10.1038/s41598-021-01545-32045-2322https://doaj.org/article/a0e6c86099b0426bb5746dc258c2ee622021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01545-3https://doaj.org/toc/2045-2322Abstract White mineral trioxide aggregate (WMTA) is a root canal treatment material, which is known to exhibit a dark brown color when in contact with sodium hypochlorite solution (NaOCl). This study aimed to investigate the effects of NaOCl on the surface properties of WMTA discs and WMTA-induced osteoblastic differentiation of periodontal ligament stem cells (PDLSCs). Mixed WMTA (ProRoot MTA) was filled into the molds to form WMTA discs. These discs were immersed in distilled water (D-WMTA) or 5% NaOCl (Na-WMTA). Their surface structures and Ca2+ release level was investigated. Moreover, they were cultured with a clonal human PDLSC line (line 1–17 cells). The main crystal structures of Na-WMTA were identical to the structures of D-WMTA. Globular aggregates with polygonal and needle-like crystals were found on D-WMTA and Na-WMTA, which included Ca, Si, Al, C and O. However, many amorphous structures were also identified on Na-WMTA. These structures consisted of Na and Cl, but did not include Ca. NaOCl immersion also reduced Ca2+ release level from whole WMTA discs. Line 1–17 cells cultured with D-WMTA formed many mineralized nodules and exhibited high expression levels of osteoblast-related genes. However, cells incubated with Na-WMTA generated a small number of nodules and showed low expression levels of osteoblast-related genes. These results indicated that NaOCl reduced Ca2+ release from WMTA by generating amorphous structures and changing its elemental distribution. NaOCl may also partially abolish the ability of WMTA to stimulate osteoblastic differentiation of PDLSCs.Kozue YamashitaAtsushi TomokiyoTaiga OnoKeita IpposhiM. Anas AlhasanAkira TsuchiyaSayuri HamanoHideki SugiiShinichiro YoshidaTomohiro ItoyamaHidefumi MaedaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kozue Yamashita Atsushi Tomokiyo Taiga Ono Keita Ipposhi M. Anas Alhasan Akira Tsuchiya Sayuri Hamano Hideki Sugii Shinichiro Yoshida Tomohiro Itoyama Hidefumi Maeda Mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells
description	Abstract White mineral trioxide aggregate (WMTA) is a root canal treatment material, which is known to exhibit a dark brown color when in contact with sodium hypochlorite solution (NaOCl). This study aimed to investigate the effects of NaOCl on the surface properties of WMTA discs and WMTA-induced osteoblastic differentiation of periodontal ligament stem cells (PDLSCs). Mixed WMTA (ProRoot MTA) was filled into the molds to form WMTA discs. These discs were immersed in distilled water (D-WMTA) or 5% NaOCl (Na-WMTA). Their surface structures and Ca2+ release level was investigated. Moreover, they were cultured with a clonal human PDLSC line (line 1–17 cells). The main crystal structures of Na-WMTA were identical to the structures of D-WMTA. Globular aggregates with polygonal and needle-like crystals were found on D-WMTA and Na-WMTA, which included Ca, Si, Al, C and O. However, many amorphous structures were also identified on Na-WMTA. These structures consisted of Na and Cl, but did not include Ca. NaOCl immersion also reduced Ca2+ release level from whole WMTA discs. Line 1–17 cells cultured with D-WMTA formed many mineralized nodules and exhibited high expression levels of osteoblast-related genes. However, cells incubated with Na-WMTA generated a small number of nodules and showed low expression levels of osteoblast-related genes. These results indicated that NaOCl reduced Ca2+ release from WMTA by generating amorphous structures and changing its elemental distribution. NaOCl may also partially abolish the ability of WMTA to stimulate osteoblastic differentiation of PDLSCs.
format	article
author	Kozue Yamashita Atsushi Tomokiyo Taiga Ono Keita Ipposhi M. Anas Alhasan Akira Tsuchiya Sayuri Hamano Hideki Sugii Shinichiro Yoshida Tomohiro Itoyama Hidefumi Maeda
author_facet	Kozue Yamashita Atsushi Tomokiyo Taiga Ono Keita Ipposhi M. Anas Alhasan Akira Tsuchiya Sayuri Hamano Hideki Sugii Shinichiro Yoshida Tomohiro Itoyama Hidefumi Maeda
author_sort	Kozue Yamashita
title	Mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells
title_short	Mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells
title_full	Mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells
title_fullStr	Mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells
title_full_unstemmed	Mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells
title_sort	mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a0e6c86099b0426bb5746dc258c2ee62
work_keys_str_mv	AT kozueyamashita mineraltrioxideaggregateimmersedinsodiumhypochloritereducetheosteoblasticdifferentiationofhumanperiodontalligamentstemcells AT atsushitomokiyo mineraltrioxideaggregateimmersedinsodiumhypochloritereducetheosteoblasticdifferentiationofhumanperiodontalligamentstemcells AT taigaono mineraltrioxideaggregateimmersedinsodiumhypochloritereducetheosteoblasticdifferentiationofhumanperiodontalligamentstemcells AT keitaipposhi mineraltrioxideaggregateimmersedinsodiumhypochloritereducetheosteoblasticdifferentiationofhumanperiodontalligamentstemcells AT manasalhasan mineraltrioxideaggregateimmersedinsodiumhypochloritereducetheosteoblasticdifferentiationofhumanperiodontalligamentstemcells AT akiratsuchiya mineraltrioxideaggregateimmersedinsodiumhypochloritereducetheosteoblasticdifferentiationofhumanperiodontalligamentstemcells AT sayurihamano mineraltrioxideaggregateimmersedinsodiumhypochloritereducetheosteoblasticdifferentiationofhumanperiodontalligamentstemcells AT hidekisugii mineraltrioxideaggregateimmersedinsodiumhypochloritereducetheosteoblasticdifferentiationofhumanperiodontalligamentstemcells AT shinichiroyoshida mineraltrioxideaggregateimmersedinsodiumhypochloritereducetheosteoblasticdifferentiationofhumanperiodontalligamentstemcells AT tomohiroitoyama mineraltrioxideaggregateimmersedinsodiumhypochloritereducetheosteoblasticdifferentiationofhumanperiodontalligamentstemcells AT hidefumimaeda mineraltrioxideaggregateimmersedinsodiumhypochloritereducetheosteoblasticdifferentiationofhumanperiodontalligamentstemcells
_version_	1718429246857150464

Mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells

Ejemplares similares