Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover

Abstract The biological pretreatment of lignocellulosic biomass is a low-cost and eco-friendly method for facilitating enzymatic hydrolysis. In this study, strains with lignin depletion capability were screened using a high-throughput screening method. Sixty-three strains were screened out and Myrot...

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Autores principales:	Yingjie Su, Xiaoxiao Yu, Yang Sun, Gang Wang, Huan Chen, Guang Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a0e8ad3e67554168b2fc8c40d60f1d67
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spelling	oai:doaj.org-article:a0e8ad3e67554168b2fc8c40d60f1d672021-12-02T15:08:12ZEvaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover10.1038/s41598-018-23626-62045-2322https://doaj.org/article/a0e8ad3e67554168b2fc8c40d60f1d672018-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-23626-6https://doaj.org/toc/2045-2322Abstract The biological pretreatment of lignocellulosic biomass is a low-cost and eco-friendly method for facilitating enzymatic hydrolysis. In this study, strains with lignin depletion capability were screened using a high-throughput screening method. Sixty-three strains were screened out and Myrothecium verrucaria secreted three lignin-degrading enzymes simultaneously during the bio-pretreatment process. The activity levels of laccase, lignin peroxidase and manganese peroxidase were 6.61, 0.78 and 1.31 U g−1 dry biomass. The content of lignin in corn stover decreased by 42.30% after bio-pretreatment, and the conversion rate increased by 123.84% during the subsequent saccharification process in comparison with the untreated corn stover. Furthermore, the effects of bio-pretreatment on the structure of corn stover were presented using a scanning electron microscope (SEM), Brunauer-Emmet-Teller (BET), X-ray diffractometer (XRD) and Fourier transform infrared spectroscopy (FTIR). The results showed that M.V. is a promising lignin-degrading fungus. This research demonstrated an efficient pretreatment approach for enhancing the enzymatic saccharification of corn stover.Yingjie SuXiaoxiao YuYang SunGang WangHuan ChenGuang ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yingjie Su Xiaoxiao Yu Yang Sun Gang Wang Huan Chen Guang Chen Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover
description	Abstract The biological pretreatment of lignocellulosic biomass is a low-cost and eco-friendly method for facilitating enzymatic hydrolysis. In this study, strains with lignin depletion capability were screened using a high-throughput screening method. Sixty-three strains were screened out and Myrothecium verrucaria secreted three lignin-degrading enzymes simultaneously during the bio-pretreatment process. The activity levels of laccase, lignin peroxidase and manganese peroxidase were 6.61, 0.78 and 1.31 U g−1 dry biomass. The content of lignin in corn stover decreased by 42.30% after bio-pretreatment, and the conversion rate increased by 123.84% during the subsequent saccharification process in comparison with the untreated corn stover. Furthermore, the effects of bio-pretreatment on the structure of corn stover were presented using a scanning electron microscope (SEM), Brunauer-Emmet-Teller (BET), X-ray diffractometer (XRD) and Fourier transform infrared spectroscopy (FTIR). The results showed that M.V. is a promising lignin-degrading fungus. This research demonstrated an efficient pretreatment approach for enhancing the enzymatic saccharification of corn stover.
format	article
author	Yingjie Su Xiaoxiao Yu Yang Sun Gang Wang Huan Chen Guang Chen
author_facet	Yingjie Su Xiaoxiao Yu Yang Sun Gang Wang Huan Chen Guang Chen
author_sort	Yingjie Su
title	Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover
title_short	Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover
title_full	Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover
title_fullStr	Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover
title_full_unstemmed	Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover
title_sort	evaluation of screened lignin-degrading fungi for the biological pretreatment of corn stover
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a0e8ad3e67554168b2fc8c40d60f1d67
work_keys_str_mv	AT yingjiesu evaluationofscreenedlignindegradingfungiforthebiologicalpretreatmentofcornstover AT xiaoxiaoyu evaluationofscreenedlignindegradingfungiforthebiologicalpretreatmentofcornstover AT yangsun evaluationofscreenedlignindegradingfungiforthebiologicalpretreatmentofcornstover AT gangwang evaluationofscreenedlignindegradingfungiforthebiologicalpretreatmentofcornstover AT huanchen evaluationofscreenedlignindegradingfungiforthebiologicalpretreatmentofcornstover AT guangchen evaluationofscreenedlignindegradingfungiforthebiologicalpretreatmentofcornstover
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Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover

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