Molecular approach to the epidemiology of urinary schistosomiasis in France.

<h4>Background</h4>The diagnosis of urogenital schistosomiasis is based on the complementarity of serological technique and microscopic examination (ME). Between 2015 and 2019, the number of urinary schistosomiasis tests received in our laboratory increased sharply from 300 to 900 per ye...

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Autores principales:	Marie-Laure Gillardie, Oussama Babba, Caroline Mahinc, Maureen Duthel, Claire de Bengy, Clotilde Morineaud, Elisabeth Rivollier, Pierre Flori
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/a0fb0456ba6c450b84b191c95dff7d51
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spelling	oai:doaj.org-article:a0fb0456ba6c450b84b191c95dff7d512021-11-25T06:33:32ZMolecular approach to the epidemiology of urinary schistosomiasis in France.1935-27271935-273510.1371/journal.pntd.0009515https://doaj.org/article/a0fb0456ba6c450b84b191c95dff7d512021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009515https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>The diagnosis of urogenital schistosomiasis is based on the complementarity of serological technique and microscopic examination (ME). Between 2015 and 2019, the number of urinary schistosomiasis tests received in our laboratory increased sharply from 300 to 900 per year. Therefore, we wanted to evaluate the reliability of urine microscopic examination (ME, reference and routine technique) from urine sample by comparing it to other techniques (antigenic technique and PCR). To this end, we optimized two real-time PCRs targeting respectively Schistosoma haematobium (Sh) and Schistosoma mansoni (Sm).<h4>Methodology/principal findings</h4>914 urine samples from 846 patients suspected of urogenital schistosomiasis were prescribed and analyzed by PCR and also by antigenic technique for the first 143 samples. The antigenic technique evaluated was Schisto POC-CCA, Rapid Medical Diagnostics. These results (antigenic technique and PCR) were compared to ME which was performed from all urines. The percentage of 14% (128/914) positive cases with the PCR technique and the percentage of 6.0% (54/914) positive cases with ME is significantly different (Chi 2 test, p<0.001). These 128 positive PCRs correspond to 120 different patients, 88.3% (106/120) of them were young migrants and 11.7% (14/120) were French patients returning from travel. Among these migrants, more than 75% (80/106) came from French-speaking West Africa. In addition, the Schisto POC-CCA showed a specificity of 39% (46/117), too poor to be used as a screening tool in low or non-endemic areas.<h4>Conclusion/significance</h4>Targeted Sh and Sm PCRs in urine are reliable techniques compared to ME (reference technique). In view of our results, we decided to screen urinary schistosomiasis by direct ME always coupled by the PCR technique, which has shown better reliability criteria.Marie-Laure GillardieOussama BabbaCaroline MahincMaureen DuthelClaire de BengyClotilde MorineaudElisabeth RivollierPierre FloriPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 7, p e0009515 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Marie-Laure Gillardie Oussama Babba Caroline Mahinc Maureen Duthel Claire de Bengy Clotilde Morineaud Elisabeth Rivollier Pierre Flori Molecular approach to the epidemiology of urinary schistosomiasis in France.
description	<h4>Background</h4>The diagnosis of urogenital schistosomiasis is based on the complementarity of serological technique and microscopic examination (ME). Between 2015 and 2019, the number of urinary schistosomiasis tests received in our laboratory increased sharply from 300 to 900 per year. Therefore, we wanted to evaluate the reliability of urine microscopic examination (ME, reference and routine technique) from urine sample by comparing it to other techniques (antigenic technique and PCR). To this end, we optimized two real-time PCRs targeting respectively Schistosoma haematobium (Sh) and Schistosoma mansoni (Sm).<h4>Methodology/principal findings</h4>914 urine samples from 846 patients suspected of urogenital schistosomiasis were prescribed and analyzed by PCR and also by antigenic technique for the first 143 samples. The antigenic technique evaluated was Schisto POC-CCA, Rapid Medical Diagnostics. These results (antigenic technique and PCR) were compared to ME which was performed from all urines. The percentage of 14% (128/914) positive cases with the PCR technique and the percentage of 6.0% (54/914) positive cases with ME is significantly different (Chi 2 test, p<0.001). These 128 positive PCRs correspond to 120 different patients, 88.3% (106/120) of them were young migrants and 11.7% (14/120) were French patients returning from travel. Among these migrants, more than 75% (80/106) came from French-speaking West Africa. In addition, the Schisto POC-CCA showed a specificity of 39% (46/117), too poor to be used as a screening tool in low or non-endemic areas.<h4>Conclusion/significance</h4>Targeted Sh and Sm PCRs in urine are reliable techniques compared to ME (reference technique). In view of our results, we decided to screen urinary schistosomiasis by direct ME always coupled by the PCR technique, which has shown better reliability criteria.
format	article
author	Marie-Laure Gillardie Oussama Babba Caroline Mahinc Maureen Duthel Claire de Bengy Clotilde Morineaud Elisabeth Rivollier Pierre Flori
author_facet	Marie-Laure Gillardie Oussama Babba Caroline Mahinc Maureen Duthel Claire de Bengy Clotilde Morineaud Elisabeth Rivollier Pierre Flori
author_sort	Marie-Laure Gillardie
title	Molecular approach to the epidemiology of urinary schistosomiasis in France.
title_short	Molecular approach to the epidemiology of urinary schistosomiasis in France.
title_full	Molecular approach to the epidemiology of urinary schistosomiasis in France.
title_fullStr	Molecular approach to the epidemiology of urinary schistosomiasis in France.
title_full_unstemmed	Molecular approach to the epidemiology of urinary schistosomiasis in France.
title_sort	molecular approach to the epidemiology of urinary schistosomiasis in france.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a0fb0456ba6c450b84b191c95dff7d51
work_keys_str_mv	AT marielauregillardie molecularapproachtotheepidemiologyofurinaryschistosomiasisinfrance AT oussamababba molecularapproachtotheepidemiologyofurinaryschistosomiasisinfrance AT carolinemahinc molecularapproachtotheepidemiologyofurinaryschistosomiasisinfrance AT maureenduthel molecularapproachtotheepidemiologyofurinaryschistosomiasisinfrance AT clairedebengy molecularapproachtotheepidemiologyofurinaryschistosomiasisinfrance AT clotildemorineaud molecularapproachtotheepidemiologyofurinaryschistosomiasisinfrance AT elisabethrivollier molecularapproachtotheepidemiologyofurinaryschistosomiasisinfrance AT pierreflori molecularapproachtotheepidemiologyofurinaryschistosomiasisinfrance
_version_	1718413637889032192

Molecular approach to the epidemiology of urinary schistosomiasis in France.

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