Repression by Cyclic AMP Receptor Protein at a Distance

ABSTRACT In a previous study of promoters dependent on the Escherichia coli cyclic AMP receptor protein (CRP), carrying tandem DNA sites for CRP, we found that the upstream-bound CRP could either enhance or repress transcription, depending on its location. Here, we have analyzed the interactions bet...

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Autores principales:	David J. Lee, Stephen J. W. Busby
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2012
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a0fb5f13ee2d4f36867640f0c7f63ac4
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spelling	oai:doaj.org-article:a0fb5f13ee2d4f36867640f0c7f63ac42021-11-15T15:39:12ZRepression by Cyclic AMP Receptor Protein at a Distance10.1128/mBio.00289-122150-7511https://doaj.org/article/a0fb5f13ee2d4f36867640f0c7f63ac42012-11-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00289-12https://doaj.org/toc/2150-7511ABSTRACT In a previous study of promoters dependent on the Escherichia coli cyclic AMP receptor protein (CRP), carrying tandem DNA sites for CRP, we found that the upstream-bound CRP could either enhance or repress transcription, depending on its location. Here, we have analyzed the interactions between CRP and the C-terminal domains of the RNA polymerase α subunits at some of these promoters. We report that the upstream-bound CRP interacts with these domains irrespective of whether it up- or downregulates promoter activity. Hence, disruption of this interaction can lead to either down- or upregulation, depending on its location. IMPORTANCE Many bacterial promoters carry multiple DNA sites for transcription factors. While most factors that downregulate promoter activity bind to targets that overlap or are downstream of the transcription start and −10 element, very few cases of repression from upstream locations have been reported. Since more Escherichia coli promoters are regulated by cyclic AMP receptor protein (CRP) than by any other transcription factor, and since multiple DNA sites for CRP are commonplace at promoters, our results suggest that promoter downregulation by transcription factors may be more prevalent than hitherto thought, and this will have implications for the annotation of promoters from new bacterial genome sequences.David J. LeeStephen J. W. BusbyAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 3, Iss 5 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 David J. Lee Stephen J. W. Busby Repression by Cyclic AMP Receptor Protein at a Distance
description	ABSTRACT In a previous study of promoters dependent on the Escherichia coli cyclic AMP receptor protein (CRP), carrying tandem DNA sites for CRP, we found that the upstream-bound CRP could either enhance or repress transcription, depending on its location. Here, we have analyzed the interactions between CRP and the C-terminal domains of the RNA polymerase α subunits at some of these promoters. We report that the upstream-bound CRP interacts with these domains irrespective of whether it up- or downregulates promoter activity. Hence, disruption of this interaction can lead to either down- or upregulation, depending on its location. IMPORTANCE Many bacterial promoters carry multiple DNA sites for transcription factors. While most factors that downregulate promoter activity bind to targets that overlap or are downstream of the transcription start and −10 element, very few cases of repression from upstream locations have been reported. Since more Escherichia coli promoters are regulated by cyclic AMP receptor protein (CRP) than by any other transcription factor, and since multiple DNA sites for CRP are commonplace at promoters, our results suggest that promoter downregulation by transcription factors may be more prevalent than hitherto thought, and this will have implications for the annotation of promoters from new bacterial genome sequences.
format	article
author	David J. Lee Stephen J. W. Busby
author_facet	David J. Lee Stephen J. W. Busby
author_sort	David J. Lee
title	Repression by Cyclic AMP Receptor Protein at a Distance
title_short	Repression by Cyclic AMP Receptor Protein at a Distance
title_full	Repression by Cyclic AMP Receptor Protein at a Distance
title_fullStr	Repression by Cyclic AMP Receptor Protein at a Distance
title_full_unstemmed	Repression by Cyclic AMP Receptor Protein at a Distance
title_sort	repression by cyclic amp receptor protein at a distance
publisher	American Society for Microbiology
publishDate	2012
url	https://doaj.org/article/a0fb5f13ee2d4f36867640f0c7f63ac4
work_keys_str_mv	AT davidjlee repressionbycyclicampreceptorproteinatadistance AT stephenjwbusby repressionbycyclicampreceptorproteinatadistance
_version_	1718427783307198464

Repression by Cyclic AMP Receptor Protein at a Distance

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