Molecular mechanism of oil induced growth inhibition in diatoms using Thalassiosira pseudonana as the model species

Abstract The 2010 Deepwater Horizon oil-spill exposed the microbes of Gulf of Mexico to unprecedented amount of oil. Conclusive evidence of the underlying molecular mechanism(s) on the negative effects of oil exposure on certain phytoplankton species such as Thalassiosira pseudonana is still lacking...

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Autores principales:	Manoj Kamalanathan, Savannah Mapes, Jessica Hillhouse, Noah Claflin, Joshua Leleux, David Hala, Antonietta Quigg
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a1071179661c4090957305b635bd7ff1
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spelling	oai:doaj.org-article:a1071179661c4090957305b635bd7ff12021-12-02T18:37:10ZMolecular mechanism of oil induced growth inhibition in diatoms using Thalassiosira pseudonana as the model species10.1038/s41598-021-98744-92045-2322https://doaj.org/article/a1071179661c4090957305b635bd7ff12021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98744-9https://doaj.org/toc/2045-2322Abstract The 2010 Deepwater Horizon oil-spill exposed the microbes of Gulf of Mexico to unprecedented amount of oil. Conclusive evidence of the underlying molecular mechanism(s) on the negative effects of oil exposure on certain phytoplankton species such as Thalassiosira pseudonana is still lacking, curtailing our understanding of how oil spills alter community composition. We performed experiments on model diatom T. pseudonana to understand the mechanisms underpinning observed reduced growth and photosynthesis rates during oil exposure. Results show severe impairment to processes upstream of photosynthesis, such as light absorption, with proteins associated with the light harvesting complex damaged while the pigments were unaffected. Proteins associated with photosynthetic electron transport were also damaged, severely affecting photosynthetic apparatus and depriving cells of energy and carbon for growth. Negative growth effects were alleviated when an organic carbon source was provided. Further investigation through proteomics combined with pathway enrichment analysis confirmed the above findings, while highlighting other negatively affected processes such as those associated with ferroxidase complex, high-affinity iron-permease complex, and multiple transmembrane transport. We also show that oxidative stress is not the primary route of negative effects, rather secondary. Overall, this study provides a mechanistic understanding of the cellular damage that occurs during oil exposure to T. pseudonana.Manoj KamalanathanSavannah MapesJessica HillhouseNoah ClaflinJoshua LeleuxDavid HalaAntonietta QuiggNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manoj Kamalanathan Savannah Mapes Jessica Hillhouse Noah Claflin Joshua Leleux David Hala Antonietta Quigg Molecular mechanism of oil induced growth inhibition in diatoms using Thalassiosira pseudonana as the model species
description	Abstract The 2010 Deepwater Horizon oil-spill exposed the microbes of Gulf of Mexico to unprecedented amount of oil. Conclusive evidence of the underlying molecular mechanism(s) on the negative effects of oil exposure on certain phytoplankton species such as Thalassiosira pseudonana is still lacking, curtailing our understanding of how oil spills alter community composition. We performed experiments on model diatom T. pseudonana to understand the mechanisms underpinning observed reduced growth and photosynthesis rates during oil exposure. Results show severe impairment to processes upstream of photosynthesis, such as light absorption, with proteins associated with the light harvesting complex damaged while the pigments were unaffected. Proteins associated with photosynthetic electron transport were also damaged, severely affecting photosynthetic apparatus and depriving cells of energy and carbon for growth. Negative growth effects were alleviated when an organic carbon source was provided. Further investigation through proteomics combined with pathway enrichment analysis confirmed the above findings, while highlighting other negatively affected processes such as those associated with ferroxidase complex, high-affinity iron-permease complex, and multiple transmembrane transport. We also show that oxidative stress is not the primary route of negative effects, rather secondary. Overall, this study provides a mechanistic understanding of the cellular damage that occurs during oil exposure to T. pseudonana.
format	article
author	Manoj Kamalanathan Savannah Mapes Jessica Hillhouse Noah Claflin Joshua Leleux David Hala Antonietta Quigg
author_facet	Manoj Kamalanathan Savannah Mapes Jessica Hillhouse Noah Claflin Joshua Leleux David Hala Antonietta Quigg
author_sort	Manoj Kamalanathan
title	Molecular mechanism of oil induced growth inhibition in diatoms using Thalassiosira pseudonana as the model species
title_short	Molecular mechanism of oil induced growth inhibition in diatoms using Thalassiosira pseudonana as the model species
title_full	Molecular mechanism of oil induced growth inhibition in diatoms using Thalassiosira pseudonana as the model species
title_fullStr	Molecular mechanism of oil induced growth inhibition in diatoms using Thalassiosira pseudonana as the model species
title_full_unstemmed	Molecular mechanism of oil induced growth inhibition in diatoms using Thalassiosira pseudonana as the model species
title_sort	molecular mechanism of oil induced growth inhibition in diatoms using thalassiosira pseudonana as the model species
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a1071179661c4090957305b635bd7ff1
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Molecular mechanism of oil induced growth inhibition in diatoms using Thalassiosira pseudonana as the model species

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