Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice

Abstract Numerous studies have shown that carbon ion radiotherapy (CIRT) induces anti-cancer immune responses in melanoma patients, yet the mechanism remains elusive. The abundance of myeloid-derived suppressor cells (MDSC) in the tumour microenvironment is associated with therapeutic efficacy and d...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Heng Zhou, Pengfei Yang, Haining Li, Liying Zhang, Jin Li, Tianyi Zhang, Chengyan Sheng, Jufang Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Publishing Group 2021
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/a10ce986536f4368886a573223230d48
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a10ce986536f4368886a573223230d48
record_format	dspace
spelling	oai:doaj.org-article:a10ce986536f4368886a573223230d482021-11-07T12:21:19ZCarbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice10.1038/s41420-021-00731-62058-7716https://doaj.org/article/a10ce986536f4368886a573223230d482021-11-01T00:00:00Zhttps://doi.org/10.1038/s41420-021-00731-6https://doaj.org/toc/2058-7716Abstract Numerous studies have shown that carbon ion radiotherapy (CIRT) induces anti-cancer immune responses in melanoma patients, yet the mechanism remains elusive. The abundance of myeloid-derived suppressor cells (MDSC) in the tumour microenvironment is associated with therapeutic efficacy and disease outcome. This study analysed the changes in the immune contexture in response to the carbon ion treatment. The murine melanoma B16, MelanA, and S91 tumour models were established in syngeneic immunocompetent mice. Then, the tumours were irradiated with carbon ion beams, and flow cytometry was utilised to observe the immune contexture changes in the bone marrow, peripheral blood, spleen, and tumours. The immune infiltrates in the tumour tissues were further assessed using haematoxylin/eosin staining and immunohistochemistry. The immunoblot detected the expression of proteins associated with the JAK/STAT signalling pathway. The secretion of immune-related cytokines was examined using ELISA. Compared to conventional radiotherapy, particle beams have distinct advantages in cancer therapy. Here, the use of carbon ion beams (5 GyE) for melanoma-bearing mice was found to reduce the population of MDSC in the bone marrow, peripheral blood, and spleen of the animals via a JAK2/STAT3-dependent mechanism. The percentage of CD3+, CD4+, CD8+ T cells, macrophages, and natural killer cells increased after radiation, resulting in reduced tumour growth and prolonged overall survival in the three different mouse models of melanoma. This study, therefore, substantiated that CIRT boosts anti-tumour immune responses via the inhibition of MDSC.Heng ZhouPengfei YangHaining LiLiying ZhangJin LiTianyi ZhangChengyan ShengJufang WangNature Publishing GrouparticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282CytologyQH573-671ENCell Death Discovery, Vol 7, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671 Heng Zhou Pengfei Yang Haining Li Liying Zhang Jin Li Tianyi Zhang Chengyan Sheng Jufang Wang Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice
description	Abstract Numerous studies have shown that carbon ion radiotherapy (CIRT) induces anti-cancer immune responses in melanoma patients, yet the mechanism remains elusive. The abundance of myeloid-derived suppressor cells (MDSC) in the tumour microenvironment is associated with therapeutic efficacy and disease outcome. This study analysed the changes in the immune contexture in response to the carbon ion treatment. The murine melanoma B16, MelanA, and S91 tumour models were established in syngeneic immunocompetent mice. Then, the tumours were irradiated with carbon ion beams, and flow cytometry was utilised to observe the immune contexture changes in the bone marrow, peripheral blood, spleen, and tumours. The immune infiltrates in the tumour tissues were further assessed using haematoxylin/eosin staining and immunohistochemistry. The immunoblot detected the expression of proteins associated with the JAK/STAT signalling pathway. The secretion of immune-related cytokines was examined using ELISA. Compared to conventional radiotherapy, particle beams have distinct advantages in cancer therapy. Here, the use of carbon ion beams (5 GyE) for melanoma-bearing mice was found to reduce the population of MDSC in the bone marrow, peripheral blood, and spleen of the animals via a JAK2/STAT3-dependent mechanism. The percentage of CD3+, CD4+, CD8+ T cells, macrophages, and natural killer cells increased after radiation, resulting in reduced tumour growth and prolonged overall survival in the three different mouse models of melanoma. This study, therefore, substantiated that CIRT boosts anti-tumour immune responses via the inhibition of MDSC.
format	article
author	Heng Zhou Pengfei Yang Haining Li Liying Zhang Jin Li Tianyi Zhang Chengyan Sheng Jufang Wang
author_facet	Heng Zhou Pengfei Yang Haining Li Liying Zhang Jin Li Tianyi Zhang Chengyan Sheng Jufang Wang
author_sort	Heng Zhou
title	Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice
title_short	Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice
title_full	Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice
title_fullStr	Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice
title_full_unstemmed	Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice
title_sort	carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice
publisher	Nature Publishing Group
publishDate	2021
url	https://doaj.org/article/a10ce986536f4368886a573223230d48
work_keys_str_mv	AT hengzhou carbonionradiotherapyboostsantitumourimmuneresponsesbyinhibitingmyeloidderivedsuppressorcellsinmelanomabearingmice AT pengfeiyang carbonionradiotherapyboostsantitumourimmuneresponsesbyinhibitingmyeloidderivedsuppressorcellsinmelanomabearingmice AT hainingli carbonionradiotherapyboostsantitumourimmuneresponsesbyinhibitingmyeloidderivedsuppressorcellsinmelanomabearingmice AT liyingzhang carbonionradiotherapyboostsantitumourimmuneresponsesbyinhibitingmyeloidderivedsuppressorcellsinmelanomabearingmice AT jinli carbonionradiotherapyboostsantitumourimmuneresponsesbyinhibitingmyeloidderivedsuppressorcellsinmelanomabearingmice AT tianyizhang carbonionradiotherapyboostsantitumourimmuneresponsesbyinhibitingmyeloidderivedsuppressorcellsinmelanomabearingmice AT chengyansheng carbonionradiotherapyboostsantitumourimmuneresponsesbyinhibitingmyeloidderivedsuppressorcellsinmelanomabearingmice AT jufangwang carbonionradiotherapyboostsantitumourimmuneresponsesbyinhibitingmyeloidderivedsuppressorcellsinmelanomabearingmice
_version_	1718443506329976832

Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice

Ejemplares similares