Secreted β3-integrin enhances natural killer cell activity against acute myeloid leukemia cells.

Integrins are a large family of heterodimeric proteins that are involved in cell adhesion, migration, and proliferation. Integrin diversity and function is regulated by alternative splicing. Membrane-bound and truncated β3-integrins were shown to be key players in cancer metastasis. However, the imm...

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Autores principales:	Younis Skaik, Stefanie Vahlsing, Lilia Goudeva, Britta Eiz-Vesper, Anja Battermann, Rainer Blasczyk, Constança Figueiredo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a10ee79f68d34974a18932fb872a6f7f
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spelling	oai:doaj.org-article:a10ee79f68d34974a18932fb872a6f7f2021-11-18T08:16:14ZSecreted β3-integrin enhances natural killer cell activity against acute myeloid leukemia cells.1932-620310.1371/journal.pone.0098936https://doaj.org/article/a10ee79f68d34974a18932fb872a6f7f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24919191/?tool=EBIhttps://doaj.org/toc/1932-6203Integrins are a large family of heterodimeric proteins that are involved in cell adhesion, migration, and proliferation. Integrin diversity and function is regulated by alternative splicing. Membrane-bound and truncated β3-integrins were shown to be key players in cancer metastasis. However, the immunomodulatory functions of the soluble (s) β3-integrin have not been investigated yet. In this study, we described a novel form of sβ3-integrin in acute myeloid leukaemia (AML) patients. Furthermore, we assessed the role of the sβ3-integrin in the modulation of natural killer (NK)-cell activity. Levels of sβ3-integrin were analysed in plasma samples of 23 AML patients and 26 healthy donors by ELISA. The capacity of sβ3-integrin to regulate NK cell activity was investigated using proliferation, cytokine secretion, and cytotoxicity assays. Circulating sβ3-integrin was detected in the plasma of 8 AML patients. NK cells showed significantly higher proliferation rates after stimulation with sβ3-integrin and IL-2, IL-15 (73%). Significant increases in the NK cells' secreted levels of TNF-α, IFN-γ were measured in presence of sβ3-integrin. In addition, sβ3-integrin caused the upregulation of Granzyme B transcripts levels as well as FasL expression levels in NK cells. Most importantly, significantly higher K562 or AML blast target cell lysis rates were observed when NK cells were exposed to sβ3-integrin. This study reports the identification of a novel sβ3-integrin in AML patients and provides novel insights into its role in the immunomodulation of NK cell activity.Younis SkaikStefanie VahlsingLilia GoudevaBritta Eiz-VesperAnja BattermannRainer BlasczykConstança FigueiredoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e98936 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Younis Skaik Stefanie Vahlsing Lilia Goudeva Britta Eiz-Vesper Anja Battermann Rainer Blasczyk Constança Figueiredo Secreted β3-integrin enhances natural killer cell activity against acute myeloid leukemia cells.
description	Integrins are a large family of heterodimeric proteins that are involved in cell adhesion, migration, and proliferation. Integrin diversity and function is regulated by alternative splicing. Membrane-bound and truncated β3-integrins were shown to be key players in cancer metastasis. However, the immunomodulatory functions of the soluble (s) β3-integrin have not been investigated yet. In this study, we described a novel form of sβ3-integrin in acute myeloid leukaemia (AML) patients. Furthermore, we assessed the role of the sβ3-integrin in the modulation of natural killer (NK)-cell activity. Levels of sβ3-integrin were analysed in plasma samples of 23 AML patients and 26 healthy donors by ELISA. The capacity of sβ3-integrin to regulate NK cell activity was investigated using proliferation, cytokine secretion, and cytotoxicity assays. Circulating sβ3-integrin was detected in the plasma of 8 AML patients. NK cells showed significantly higher proliferation rates after stimulation with sβ3-integrin and IL-2, IL-15 (73%). Significant increases in the NK cells' secreted levels of TNF-α, IFN-γ were measured in presence of sβ3-integrin. In addition, sβ3-integrin caused the upregulation of Granzyme B transcripts levels as well as FasL expression levels in NK cells. Most importantly, significantly higher K562 or AML blast target cell lysis rates were observed when NK cells were exposed to sβ3-integrin. This study reports the identification of a novel sβ3-integrin in AML patients and provides novel insights into its role in the immunomodulation of NK cell activity.
format	article
author	Younis Skaik Stefanie Vahlsing Lilia Goudeva Britta Eiz-Vesper Anja Battermann Rainer Blasczyk Constança Figueiredo
author_facet	Younis Skaik Stefanie Vahlsing Lilia Goudeva Britta Eiz-Vesper Anja Battermann Rainer Blasczyk Constança Figueiredo
author_sort	Younis Skaik
title	Secreted β3-integrin enhances natural killer cell activity against acute myeloid leukemia cells.
title_short	Secreted β3-integrin enhances natural killer cell activity against acute myeloid leukemia cells.
title_full	Secreted β3-integrin enhances natural killer cell activity against acute myeloid leukemia cells.
title_fullStr	Secreted β3-integrin enhances natural killer cell activity against acute myeloid leukemia cells.
title_full_unstemmed	Secreted β3-integrin enhances natural killer cell activity against acute myeloid leukemia cells.
title_sort	secreted β3-integrin enhances natural killer cell activity against acute myeloid leukemia cells.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/a10ee79f68d34974a18932fb872a6f7f
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Secreted β3-integrin enhances natural killer cell activity against acute myeloid leukemia cells.

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