Direct electrochemical measurement of metanephrines in spot urine samples for the diagnosis of phaeochromocytomas

Abstract Metanephrines (MNs) were suggested as a potential first-line biochemical index for the diagnosis of phaeochromocytomas (PHEO). In this study, we developed a simple electrochemical method for the quantitative measurement of MNs in spot urine samples. As MNs contain a hydroxyphenyl group, the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zheng-Hu Shi, Xiao-Qing Zhang, Qian-Na Zhen, Ming Zuo, Gang Tian, Yi-Fan He, Min Ding
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a10fd7a6c5ad4da6b0e4eba530f9f23f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a10fd7a6c5ad4da6b0e4eba530f9f23f
record_format	dspace
spelling	oai:doaj.org-article:a10fd7a6c5ad4da6b0e4eba530f9f23f2021-12-02T11:52:44ZDirect electrochemical measurement of metanephrines in spot urine samples for the diagnosis of phaeochromocytomas10.1038/s41598-017-08612-82045-2322https://doaj.org/article/a10fd7a6c5ad4da6b0e4eba530f9f23f2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08612-8https://doaj.org/toc/2045-2322Abstract Metanephrines (MNs) were suggested as a potential first-line biochemical index for the diagnosis of phaeochromocytomas (PHEO). In this study, we developed a simple electrochemical method for the quantitative measurement of MNs in spot urine samples. As MNs contain a hydroxyphenyl group, they could be oxidized at a certain potential to quinines, which could be further detected by the differential pulse voltammetry (DPV) method using unmodified screen-printed carbon electrode (SPCE). Meanwhile, the solid phase extraction (SPE) technique was used to eliminate the matrix effect in the samples. Consequently, free MNs from the extracted urine sample were screened in a linear range from 0.25 mg/L to 12.5 mg/L. The lowest limit of quantification (LLOQ) for MNs was 0.25 mg/L and the limit of detection (LOD) was 0.05 mg/L. Both the precisions and recoveries were sufficient for clinical applications. The urine samples from 22 patients with PHEO and 63 controls were analyzed by the proposed method. The area under the ROC curve was 0.981 (95% CI, 0.958–1.000) with the sensitivity of 95.5% and the specificity of 92.4% at the cut-off value of 0.404 mg/L in these urine samples. Overall, the proposed method provides a cost-effective, rapid and simple tool for clinical diagnosis of PHEO.Zheng-Hu ShiXiao-Qing ZhangQian-Na ZhenMing ZuoGang TianYi-Fan HeMin DingNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zheng-Hu Shi Xiao-Qing Zhang Qian-Na Zhen Ming Zuo Gang Tian Yi-Fan He Min Ding Direct electrochemical measurement of metanephrines in spot urine samples for the diagnosis of phaeochromocytomas
description	Abstract Metanephrines (MNs) were suggested as a potential first-line biochemical index for the diagnosis of phaeochromocytomas (PHEO). In this study, we developed a simple electrochemical method for the quantitative measurement of MNs in spot urine samples. As MNs contain a hydroxyphenyl group, they could be oxidized at a certain potential to quinines, which could be further detected by the differential pulse voltammetry (DPV) method using unmodified screen-printed carbon electrode (SPCE). Meanwhile, the solid phase extraction (SPE) technique was used to eliminate the matrix effect in the samples. Consequently, free MNs from the extracted urine sample were screened in a linear range from 0.25 mg/L to 12.5 mg/L. The lowest limit of quantification (LLOQ) for MNs was 0.25 mg/L and the limit of detection (LOD) was 0.05 mg/L. Both the precisions and recoveries were sufficient for clinical applications. The urine samples from 22 patients with PHEO and 63 controls were analyzed by the proposed method. The area under the ROC curve was 0.981 (95% CI, 0.958–1.000) with the sensitivity of 95.5% and the specificity of 92.4% at the cut-off value of 0.404 mg/L in these urine samples. Overall, the proposed method provides a cost-effective, rapid and simple tool for clinical diagnosis of PHEO.
format	article
author	Zheng-Hu Shi Xiao-Qing Zhang Qian-Na Zhen Ming Zuo Gang Tian Yi-Fan He Min Ding
author_facet	Zheng-Hu Shi Xiao-Qing Zhang Qian-Na Zhen Ming Zuo Gang Tian Yi-Fan He Min Ding
author_sort	Zheng-Hu Shi
title	Direct electrochemical measurement of metanephrines in spot urine samples for the diagnosis of phaeochromocytomas
title_short	Direct electrochemical measurement of metanephrines in spot urine samples for the diagnosis of phaeochromocytomas
title_full	Direct electrochemical measurement of metanephrines in spot urine samples for the diagnosis of phaeochromocytomas
title_fullStr	Direct electrochemical measurement of metanephrines in spot urine samples for the diagnosis of phaeochromocytomas
title_full_unstemmed	Direct electrochemical measurement of metanephrines in spot urine samples for the diagnosis of phaeochromocytomas
title_sort	direct electrochemical measurement of metanephrines in spot urine samples for the diagnosis of phaeochromocytomas
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a10fd7a6c5ad4da6b0e4eba530f9f23f
work_keys_str_mv	AT zhenghushi directelectrochemicalmeasurementofmetanephrinesinspoturinesamplesforthediagnosisofphaeochromocytomas AT xiaoqingzhang directelectrochemicalmeasurementofmetanephrinesinspoturinesamplesforthediagnosisofphaeochromocytomas AT qiannazhen directelectrochemicalmeasurementofmetanephrinesinspoturinesamplesforthediagnosisofphaeochromocytomas AT mingzuo directelectrochemicalmeasurementofmetanephrinesinspoturinesamplesforthediagnosisofphaeochromocytomas AT gangtian directelectrochemicalmeasurementofmetanephrinesinspoturinesamplesforthediagnosisofphaeochromocytomas AT yifanhe directelectrochemicalmeasurementofmetanephrinesinspoturinesamplesforthediagnosisofphaeochromocytomas AT minding directelectrochemicalmeasurementofmetanephrinesinspoturinesamplesforthediagnosisofphaeochromocytomas
_version_	1718394942200479744

Direct electrochemical measurement of metanephrines in spot urine samples for the diagnosis of phaeochromocytomas

Ejemplares similares