Transforming a pair of orthogonal tRNA-aminoacyl-tRNA synthetase from Archaea to function in mammalian cells.

A previously engineered Methanocaldococcus jannaschii tRNA(CUA Tyr)-tyrosyl-tRNA synthetase pair orthogonal to Escherichia coli was modified to become orthogonal in mammalian cells. The resulting tRNA(CUA Tyr)-tyrosyl-tRNA synthetase pair was able to suppress an amber codon in the green fluorescent...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gabrielle Nina Thibodeaux, Xiang Liang, Kathryn Moncivais, Aiko Umeda, Oded Singer, Lital Alfonta, Zhiwen Jonathan Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a1127cd8f5f447949c89c2e572599f23
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a1127cd8f5f447949c89c2e572599f23
record_format	dspace
spelling	oai:doaj.org-article:a1127cd8f5f447949c89c2e572599f232021-12-02T20:20:36ZTransforming a pair of orthogonal tRNA-aminoacyl-tRNA synthetase from Archaea to function in mammalian cells.1932-620310.1371/journal.pone.0011263https://doaj.org/article/a1127cd8f5f447949c89c2e572599f232010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20582317/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203A previously engineered Methanocaldococcus jannaschii tRNA(CUA Tyr)-tyrosyl-tRNA synthetase pair orthogonal to Escherichia coli was modified to become orthogonal in mammalian cells. The resulting tRNA(CUA Tyr)-tyrosyl-tRNA synthetase pair was able to suppress an amber codon in the green fluorescent protein, GFP, and in a foldon protein in mammalian cells. The methodology reported here will allow rapid transformation of the much larger collection of existing tyrosyl-tRNA synthetases that were already evolved for the incorporation of an array of over 50 unnatural amino acids into proteins in Escherichia coli into proteins in mammalian cells. Thus we will be able to introduce a large array of possibilities for protein modifications in mammalian cells.Gabrielle Nina ThibodeauxXiang LiangKathryn MoncivaisAiko UmedaOded SingerLital AlfontaZhiwen Jonathan ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 6, p e11263 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gabrielle Nina Thibodeaux Xiang Liang Kathryn Moncivais Aiko Umeda Oded Singer Lital Alfonta Zhiwen Jonathan Zhang Transforming a pair of orthogonal tRNA-aminoacyl-tRNA synthetase from Archaea to function in mammalian cells.
description	A previously engineered Methanocaldococcus jannaschii tRNA(CUA Tyr)-tyrosyl-tRNA synthetase pair orthogonal to Escherichia coli was modified to become orthogonal in mammalian cells. The resulting tRNA(CUA Tyr)-tyrosyl-tRNA synthetase pair was able to suppress an amber codon in the green fluorescent protein, GFP, and in a foldon protein in mammalian cells. The methodology reported here will allow rapid transformation of the much larger collection of existing tyrosyl-tRNA synthetases that were already evolved for the incorporation of an array of over 50 unnatural amino acids into proteins in Escherichia coli into proteins in mammalian cells. Thus we will be able to introduce a large array of possibilities for protein modifications in mammalian cells.
format	article
author	Gabrielle Nina Thibodeaux Xiang Liang Kathryn Moncivais Aiko Umeda Oded Singer Lital Alfonta Zhiwen Jonathan Zhang
author_facet	Gabrielle Nina Thibodeaux Xiang Liang Kathryn Moncivais Aiko Umeda Oded Singer Lital Alfonta Zhiwen Jonathan Zhang
author_sort	Gabrielle Nina Thibodeaux
title	Transforming a pair of orthogonal tRNA-aminoacyl-tRNA synthetase from Archaea to function in mammalian cells.
title_short	Transforming a pair of orthogonal tRNA-aminoacyl-tRNA synthetase from Archaea to function in mammalian cells.
title_full	Transforming a pair of orthogonal tRNA-aminoacyl-tRNA synthetase from Archaea to function in mammalian cells.
title_fullStr	Transforming a pair of orthogonal tRNA-aminoacyl-tRNA synthetase from Archaea to function in mammalian cells.
title_full_unstemmed	Transforming a pair of orthogonal tRNA-aminoacyl-tRNA synthetase from Archaea to function in mammalian cells.
title_sort	transforming a pair of orthogonal trna-aminoacyl-trna synthetase from archaea to function in mammalian cells.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/a1127cd8f5f447949c89c2e572599f23
work_keys_str_mv	AT gabrielleninathibodeaux transformingapairoforthogonaltrnaaminoacyltrnasynthetasefromarchaeatofunctioninmammaliancells AT xiangliang transformingapairoforthogonaltrnaaminoacyltrnasynthetasefromarchaeatofunctioninmammaliancells AT kathrynmoncivais transformingapairoforthogonaltrnaaminoacyltrnasynthetasefromarchaeatofunctioninmammaliancells AT aikoumeda transformingapairoforthogonaltrnaaminoacyltrnasynthetasefromarchaeatofunctioninmammaliancells AT odedsinger transformingapairoforthogonaltrnaaminoacyltrnasynthetasefromarchaeatofunctioninmammaliancells AT litalalfonta transformingapairoforthogonaltrnaaminoacyltrnasynthetasefromarchaeatofunctioninmammaliancells AT zhiwenjonathanzhang transformingapairoforthogonaltrnaaminoacyltrnasynthetasefromarchaeatofunctioninmammaliancells
_version_	1718374180557160448

Transforming a pair of orthogonal tRNA-aminoacyl-tRNA synthetase from Archaea to function in mammalian cells.

Ejemplares similares