Key Cannabis Salt-Responsive Genes and Pathways Revealed by Comparative Transcriptome and Physiological Analyses of Contrasting Varieties

For the dissection and identification of the molecular response mechanisms to salt stress in cannabis, an experiment was conducted surveying the diversity of physiological characteristics. RNA-seq profiling was carried out to identify differential expression genes and pathway which respond to salt s...

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Autores principales:	Jiangjiang Zhang, Cuiping Zhang, Siqi Huang, Li Chang, Jianjun Li, Huijuan Tang, Susmita Dey, Ashok Biswas, Dengxiang Du, Defang Li, Lining Zhao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cannabis differentially expressed genes salt stress transcriptome MAPK Agriculture S
Acceso en línea:	https://doaj.org/article/a114e6a202ce48d3a23036ae0a63ce4e
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spelling	oai:doaj.org-article:a114e6a202ce48d3a23036ae0a63ce4e2021-11-25T16:11:59ZKey Cannabis Salt-Responsive Genes and Pathways Revealed by Comparative Transcriptome and Physiological Analyses of Contrasting Varieties10.3390/agronomy111123382073-4395https://doaj.org/article/a114e6a202ce48d3a23036ae0a63ce4e2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2338https://doaj.org/toc/2073-4395For the dissection and identification of the molecular response mechanisms to salt stress in cannabis, an experiment was conducted surveying the diversity of physiological characteristics. RNA-seq profiling was carried out to identify differential expression genes and pathway which respond to salt stress in different cannabis materials. The result of physiological diversity analyses showed that it is more sensitive to proline contents in K94 than in W20; 6 h was needed to reach the maximum in K94, compared to 12 h in W20. For profiling 0–72 h after treatment, a total of 10,149 differentially expressed genes were identified, and 249 genes exhibited significantly diverse expression levels in K94, which were clustered in plant hormone signal transduction and the MAPK signaling pathway. A total of 371 genes showed significant diversity expression variations in W20, which were clustered in the phenylpropanoid biosynthesis and plant hormone signal transduction pathway. The pathway enrichment by genes which were identified in K94 and W20 showed a similar trend to those clustered in plant hormone signal transduction pathways and MAPK signaling. Otherwise, there were 85 genes which identified overlaps between the two materials, indicating that these may be underlying genes related to salt stress in cannabis. The 86.67% agreement of the RNA-seq and qRT-PCR indicated the accuracy and reliability of the RNA-seq technique. Additionally, the result of physiological diversity was consistent with the predicted RNA-seq-based findings. This research may offer new insights into the molecular networks mediating cannabis to respond to salt stress.Jiangjiang ZhangCuiping ZhangSiqi HuangLi ChangJianjun LiHuijuan TangSusmita DeyAshok BiswasDengxiang DuDefang LiLining ZhaoMDPI AGarticlecannabisdifferentially expressed genessalt stresstranscriptomeMAPKAgricultureSENAgronomy, Vol 11, Iss 2338, p 2338 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cannabis differentially expressed genes salt stress transcriptome MAPK Agriculture S
spellingShingle	cannabis differentially expressed genes salt stress transcriptome MAPK Agriculture S Jiangjiang Zhang Cuiping Zhang Siqi Huang Li Chang Jianjun Li Huijuan Tang Susmita Dey Ashok Biswas Dengxiang Du Defang Li Lining Zhao Key Cannabis Salt-Responsive Genes and Pathways Revealed by Comparative Transcriptome and Physiological Analyses of Contrasting Varieties
description	For the dissection and identification of the molecular response mechanisms to salt stress in cannabis, an experiment was conducted surveying the diversity of physiological characteristics. RNA-seq profiling was carried out to identify differential expression genes and pathway which respond to salt stress in different cannabis materials. The result of physiological diversity analyses showed that it is more sensitive to proline contents in K94 than in W20; 6 h was needed to reach the maximum in K94, compared to 12 h in W20. For profiling 0–72 h after treatment, a total of 10,149 differentially expressed genes were identified, and 249 genes exhibited significantly diverse expression levels in K94, which were clustered in plant hormone signal transduction and the MAPK signaling pathway. A total of 371 genes showed significant diversity expression variations in W20, which were clustered in the phenylpropanoid biosynthesis and plant hormone signal transduction pathway. The pathway enrichment by genes which were identified in K94 and W20 showed a similar trend to those clustered in plant hormone signal transduction pathways and MAPK signaling. Otherwise, there were 85 genes which identified overlaps between the two materials, indicating that these may be underlying genes related to salt stress in cannabis. The 86.67% agreement of the RNA-seq and qRT-PCR indicated the accuracy and reliability of the RNA-seq technique. Additionally, the result of physiological diversity was consistent with the predicted RNA-seq-based findings. This research may offer new insights into the molecular networks mediating cannabis to respond to salt stress.
format	article
author	Jiangjiang Zhang Cuiping Zhang Siqi Huang Li Chang Jianjun Li Huijuan Tang Susmita Dey Ashok Biswas Dengxiang Du Defang Li Lining Zhao
author_facet	Jiangjiang Zhang Cuiping Zhang Siqi Huang Li Chang Jianjun Li Huijuan Tang Susmita Dey Ashok Biswas Dengxiang Du Defang Li Lining Zhao
author_sort	Jiangjiang Zhang
title	Key Cannabis Salt-Responsive Genes and Pathways Revealed by Comparative Transcriptome and Physiological Analyses of Contrasting Varieties
title_short	Key Cannabis Salt-Responsive Genes and Pathways Revealed by Comparative Transcriptome and Physiological Analyses of Contrasting Varieties
title_full	Key Cannabis Salt-Responsive Genes and Pathways Revealed by Comparative Transcriptome and Physiological Analyses of Contrasting Varieties
title_fullStr	Key Cannabis Salt-Responsive Genes and Pathways Revealed by Comparative Transcriptome and Physiological Analyses of Contrasting Varieties
title_full_unstemmed	Key Cannabis Salt-Responsive Genes and Pathways Revealed by Comparative Transcriptome and Physiological Analyses of Contrasting Varieties
title_sort	key cannabis salt-responsive genes and pathways revealed by comparative transcriptome and physiological analyses of contrasting varieties
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a114e6a202ce48d3a23036ae0a63ce4e
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Key Cannabis Salt-Responsive Genes and Pathways Revealed by Comparative Transcriptome and Physiological Analyses of Contrasting Varieties

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