Metabolic engineering of Escherichia coli for biosynthesis of β‐nicotinamide mononucleotide from nicotinamide

Summary The β‐nicotinamide mononucleotide (NMN) is a key intermediate of an essential coenzyme for cellular redox reactions, NAD. Administration of NMN is reported to improve various symptoms, such as diabetes and age‐related physiological decline. Thus, NMN is attracting much attention as a promisi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yang Liu, Montri Yasawong, Bo Yu
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/a1185ff7edf8420095876a8e05f87a2f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a1185ff7edf8420095876a8e05f87a2f
record_format	dspace
spelling	oai:doaj.org-article:a1185ff7edf8420095876a8e05f87a2f2021-11-18T15:39:53ZMetabolic engineering of Escherichia coli for biosynthesis of β‐nicotinamide mononucleotide from nicotinamide1751-791510.1111/1751-7915.13901https://doaj.org/article/a1185ff7edf8420095876a8e05f87a2f2021-11-01T00:00:00Zhttps://doi.org/10.1111/1751-7915.13901https://doaj.org/toc/1751-7915Summary The β‐nicotinamide mononucleotide (NMN) is a key intermediate of an essential coenzyme for cellular redox reactions, NAD. Administration of NMN is reported to improve various symptoms, such as diabetes and age‐related physiological decline. Thus, NMN is attracting much attention as a promising nutraceutical. Here, we engineered an Escherichia coli strain to produce NMN from cheap substrate nicotinamide (NAM) and glucose. The supply of in vivo precursor phosphoribosyl pyrophosphate (PRPP) and ATP was enhanced by strengthening the metabolic flux from glucose. A nicotinamide phosphoribosyltransferase with high activity was newly screened, which is the key enzyme for converting NAM to NMN with PRPP as cofactor. Notably, the E. coli endogenous protein YgcS, which function is primarily in the uptake of sugars, was firstly proven to be beneficial for NMN production in this study. Fine‐tuning regulation of ygcS gene expression in the engineered E. coli strain increased NMN production. Combined with process optimization of whole‐cell biocatalysts reaction, a final NMN titre of 496.2 mg l‐1 was obtained.Yang LiuMontri YasawongBo YuWileyarticleBiotechnologyTP248.13-248.65ENMicrobial Biotechnology, Vol 14, Iss 6, Pp 2581-2591 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65
spellingShingle	Biotechnology TP248.13-248.65 Yang Liu Montri Yasawong Bo Yu Metabolic engineering of Escherichia coli for biosynthesis of β‐nicotinamide mononucleotide from nicotinamide
description	Summary The β‐nicotinamide mononucleotide (NMN) is a key intermediate of an essential coenzyme for cellular redox reactions, NAD. Administration of NMN is reported to improve various symptoms, such as diabetes and age‐related physiological decline. Thus, NMN is attracting much attention as a promising nutraceutical. Here, we engineered an Escherichia coli strain to produce NMN from cheap substrate nicotinamide (NAM) and glucose. The supply of in vivo precursor phosphoribosyl pyrophosphate (PRPP) and ATP was enhanced by strengthening the metabolic flux from glucose. A nicotinamide phosphoribosyltransferase with high activity was newly screened, which is the key enzyme for converting NAM to NMN with PRPP as cofactor. Notably, the E. coli endogenous protein YgcS, which function is primarily in the uptake of sugars, was firstly proven to be beneficial for NMN production in this study. Fine‐tuning regulation of ygcS gene expression in the engineered E. coli strain increased NMN production. Combined with process optimization of whole‐cell biocatalysts reaction, a final NMN titre of 496.2 mg l‐1 was obtained.
format	article
author	Yang Liu Montri Yasawong Bo Yu
author_facet	Yang Liu Montri Yasawong Bo Yu
author_sort	Yang Liu
title	Metabolic engineering of Escherichia coli for biosynthesis of β‐nicotinamide mononucleotide from nicotinamide
title_short	Metabolic engineering of Escherichia coli for biosynthesis of β‐nicotinamide mononucleotide from nicotinamide
title_full	Metabolic engineering of Escherichia coli for biosynthesis of β‐nicotinamide mononucleotide from nicotinamide
title_fullStr	Metabolic engineering of Escherichia coli for biosynthesis of β‐nicotinamide mononucleotide from nicotinamide
title_full_unstemmed	Metabolic engineering of Escherichia coli for biosynthesis of β‐nicotinamide mononucleotide from nicotinamide
title_sort	metabolic engineering of escherichia coli for biosynthesis of β‐nicotinamide mononucleotide from nicotinamide
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/a1185ff7edf8420095876a8e05f87a2f
work_keys_str_mv	AT yangliu metabolicengineeringofescherichiacoliforbiosynthesisofbnicotinamidemononucleotidefromnicotinamide AT montriyasawong metabolicengineeringofescherichiacoliforbiosynthesisofbnicotinamidemononucleotidefromnicotinamide AT boyu metabolicengineeringofescherichiacoliforbiosynthesisofbnicotinamidemononucleotidefromnicotinamide
_version_	1718420801321959424

Metabolic engineering of Escherichia coli for biosynthesis of β‐nicotinamide mononucleotide from nicotinamide

Ejemplares similares