Exosomes derived from microRNA-129-5p-modified tumor cells selectively enhanced suppressive effect in malignant behaviors of homologous colon cancer cells

Exosome-encapsulated microRNAs (miRNAs) are novel diagnostic and predictive markers in colon cancer. Hence, the study of serum exosomal miRNAs in patients with colon cancer may help its diagnosis and treatment. PKH26-labeled exosomal uptake analysis identified whether exosomes transfer miRNA-129-5p...

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Autores principales:	Yong Ji, Jianxiang Ji, Hongming Yin, Xu Chen, Pengcheng Zhao, Huahu Lu, Taowu Wang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	homologous colon cancer exosomes mirna-129-5p gene expression molecular mechanism Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/a11d824343964ef1ab2de14ff87ed8e4
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spelling	oai:doaj.org-article:a11d824343964ef1ab2de14ff87ed8e42021-11-17T14:21:59ZExosomes derived from microRNA-129-5p-modified tumor cells selectively enhanced suppressive effect in malignant behaviors of homologous colon cancer cells2165-59792165-598710.1080/21655979.2021.2004981https://doaj.org/article/a11d824343964ef1ab2de14ff87ed8e42021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2004981https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Exosome-encapsulated microRNAs (miRNAs) are novel diagnostic and predictive markers in colon cancer. Hence, the study of serum exosomal miRNAs in patients with colon cancer may help its diagnosis and treatment. PKH26-labeled exosomal uptake analysis identified whether exosomes transfer miRNA-129-5p to target cells. Transmission electron microscopy and dynamic light scattering analysis were applied to determine exosome morphology and size distribution. The Cell Counting Kit-8, wound healing assay and Transwell assays were used to detect cell proliferation, migration, and invasion after treatment with engineered exosomes. Moreover, the western blotting was used to quantify the expression of proteins involved in cell apoptosis. In our study, hepatocellular liver carcinoma, cervical cancer and colon cancer cells were selected as the target cells of miRNA-129-5p exosomes. Exosomes containing miRNA-129-5p were found to be significantly more easily absorbed by colon cancer cells, presenting a stronger inhibitory effect on colon cancer cell proliferation. MiRNA-129-5p exosomes induced apoptosis in colon cancer cells while inhibiting their proliferation, migration, and invasion. In conclusion, exosomes derived from miRNA-129-5p-modified tumor cells selectively inhibited colon cancer progression, shedding new insights to therapeutic efficacy of this cancer.Yong JiJianxiang JiHongming YinXu ChenPengcheng ZhaoHuahu LuTaowu WangTaylor & Francis Grouparticlehomologous colon cancerexosomesmirna-129-5pgene expressionmolecular mechanismBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	homologous colon cancer exosomes mirna-129-5p gene expression molecular mechanism Biotechnology TP248.13-248.65
spellingShingle	homologous colon cancer exosomes mirna-129-5p gene expression molecular mechanism Biotechnology TP248.13-248.65 Yong Ji Jianxiang Ji Hongming Yin Xu Chen Pengcheng Zhao Huahu Lu Taowu Wang Exosomes derived from microRNA-129-5p-modified tumor cells selectively enhanced suppressive effect in malignant behaviors of homologous colon cancer cells
description	Exosome-encapsulated microRNAs (miRNAs) are novel diagnostic and predictive markers in colon cancer. Hence, the study of serum exosomal miRNAs in patients with colon cancer may help its diagnosis and treatment. PKH26-labeled exosomal uptake analysis identified whether exosomes transfer miRNA-129-5p to target cells. Transmission electron microscopy and dynamic light scattering analysis were applied to determine exosome morphology and size distribution. The Cell Counting Kit-8, wound healing assay and Transwell assays were used to detect cell proliferation, migration, and invasion after treatment with engineered exosomes. Moreover, the western blotting was used to quantify the expression of proteins involved in cell apoptosis. In our study, hepatocellular liver carcinoma, cervical cancer and colon cancer cells were selected as the target cells of miRNA-129-5p exosomes. Exosomes containing miRNA-129-5p were found to be significantly more easily absorbed by colon cancer cells, presenting a stronger inhibitory effect on colon cancer cell proliferation. MiRNA-129-5p exosomes induced apoptosis in colon cancer cells while inhibiting their proliferation, migration, and invasion. In conclusion, exosomes derived from miRNA-129-5p-modified tumor cells selectively inhibited colon cancer progression, shedding new insights to therapeutic efficacy of this cancer.
format	article
author	Yong Ji Jianxiang Ji Hongming Yin Xu Chen Pengcheng Zhao Huahu Lu Taowu Wang
author_facet	Yong Ji Jianxiang Ji Hongming Yin Xu Chen Pengcheng Zhao Huahu Lu Taowu Wang
author_sort	Yong Ji
title	Exosomes derived from microRNA-129-5p-modified tumor cells selectively enhanced suppressive effect in malignant behaviors of homologous colon cancer cells
title_short	Exosomes derived from microRNA-129-5p-modified tumor cells selectively enhanced suppressive effect in malignant behaviors of homologous colon cancer cells
title_full	Exosomes derived from microRNA-129-5p-modified tumor cells selectively enhanced suppressive effect in malignant behaviors of homologous colon cancer cells
title_fullStr	Exosomes derived from microRNA-129-5p-modified tumor cells selectively enhanced suppressive effect in malignant behaviors of homologous colon cancer cells
title_full_unstemmed	Exosomes derived from microRNA-129-5p-modified tumor cells selectively enhanced suppressive effect in malignant behaviors of homologous colon cancer cells
title_sort	exosomes derived from microrna-129-5p-modified tumor cells selectively enhanced suppressive effect in malignant behaviors of homologous colon cancer cells
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/a11d824343964ef1ab2de14ff87ed8e4
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_version_	1718425433052020736

Exosomes derived from microRNA-129-5p-modified tumor cells selectively enhanced suppressive effect in malignant behaviors of homologous colon cancer cells

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