Nanotoxic Effects of Silver Nanoparticles on Normal HEK-293 Cells in Comparison to Cancerous HeLa Cell Line

Xiongwei Liu,1,* Kuizhong Shan,2,* Xiaxia Shao,1 Xianqing Shi,3 Yun He,4 Zhen Liu,1 Joe Antony Jacob,5 Lichun Deng1 1Department of Oncology, Affiliated Jiangyin Hospital of Medical College, Southeast University, Jiangyin, People’s Republic of China; 2Department of Oncology, The Second Peop...

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Autores principales:	Liu X, Shan K, Shao X, Shi X, He Y, Liu Z, Jacob JA, Deng L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	agnps xrd hek-293 hela nanotoxicity. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a11dd5d5b2494ed68e028f89d827a82b
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spelling	oai:doaj.org-article:a11dd5d5b2494ed68e028f89d827a82b2021-12-02T13:50:57ZNanotoxic Effects of Silver Nanoparticles on Normal HEK-293 Cells in Comparison to Cancerous HeLa Cell Line1178-2013https://doaj.org/article/a11dd5d5b2494ed68e028f89d827a82b2021-02-01T00:00:00Zhttps://www.dovepress.com/nanotoxic-effects-of-silver-nanoparticles-on-normal-hek-293-cells-in-c-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xiongwei Liu,1,* Kuizhong Shan,2,* Xiaxia Shao,1 Xianqing Shi,3 Yun He,4 Zhen Liu,1 Joe Antony Jacob,5 Lichun Deng1 1Department of Oncology, Affiliated Jiangyin Hospital of Medical College, Southeast University, Jiangyin, People’s Republic of China; 2Department of Oncology, The Second People’s Hospital of Kunshan, Kunshan, People’s Republic of China; 3Department of Oncology, Liyang People’s Hospital, Liyang, People’s Republic of China; 4Department of Oncology, Affiliated Hospital of Chinese Medicine of Changshu City, Nanjing University of Chinese Medicine, Changshu, People’s Republic of China; 5Nanosynthesis Unit, Nanome Consulting, Salem, Tamil Nadu, India*These authors contributed equally to this workCorrespondence: Lichun DengDepartment of Oncology, Affiliated Jiangyin Hospital of Medical College, Southeast University, No. 163 Shoushan Road, Jiangyin, 214400, Jiangsu Province, People’s Republic of ChinaTel +86 510 8061 5131Email 18921233265@163.comJoe Antony JacobNanosynthesis Unit, Nanome Consulting, No. 11, Kannankurichi Road, Salem, 636008, Tamil Nadu, IndiaTel +91-9443861940Email joeantonyjacob@gmail.comPurpose: Biomimetic approaches for the synthesis of silver nanoparticles (AgNPs) had created a substantial impression among the research community that focuses on nano-bio interactions. In this study, an eco-friendly method using Rhizophora apiculata aqueous leaf extract as a reductant-rich hydrosol was followed to synthesize AgNPs and test its cytotoxicity.Methods: To optimise the parameters for the synthesis of AgNPs, central composite design based on response surface methodology was used. The particles synthesized at a nano-scale were characterized in our previously published report. The present report further characterizes the nanoparticles by X-ray diffraction, SEM and TEM at varying sites and magnifications. The characterized AgNPs were tested for their cytotoxic effects on HEK-293 and HeLa cells.Results: The cytotoxicity on the cell lines was dose-dependent. At a concentration of 2.5 μL/mL of the AgNPs-containing hydrosol, 100% inhibition of HEK-293 cells and 75% inhibition of the HeLa cells were observed. The IC50 value for AgNPs on HEK-293 was 0.622 μL/mL (12.135 ng), whereas, for HeLa cells, it was 1.98 μL/mL (38.629 ng).Conclusion: The nanoparticles were three-fold toxic towards the HEK-293 cells in comparison to the HeLa cells. Therefore, the therapeutic index is low for R. apiculata derived AgNPs on HeLa cells when tested in comparison with the HEK-293 cells. The nanotoxicity profile of the synthesized AgNPs seems more prominent than the nanotherapeutic index. According to our knowledge, this is the first-ever report on the optimization of synthesis of AgNPs using response surface methodology and identifying the therapeutic index of mangrove leaf-derived AgNPs.Keywords: AgNPs, XRD, HEK-293, HeLa, nanotoxicityLiu XShan KShao XShi XHe YLiu ZJacob JADeng LDove Medical Pressarticleagnpsxrdhek-293helananotoxicity.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 753-761 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	agnps xrd hek-293 hela nanotoxicity. Medicine (General) R5-920
spellingShingle	agnps xrd hek-293 hela nanotoxicity. Medicine (General) R5-920 Liu X Shan K Shao X Shi X He Y Liu Z Jacob JA Deng L Nanotoxic Effects of Silver Nanoparticles on Normal HEK-293 Cells in Comparison to Cancerous HeLa Cell Line
description	Xiongwei Liu,1,* Kuizhong Shan,2,* Xiaxia Shao,1 Xianqing Shi,3 Yun He,4 Zhen Liu,1 Joe Antony Jacob,5 Lichun Deng1 1Department of Oncology, Affiliated Jiangyin Hospital of Medical College, Southeast University, Jiangyin, People’s Republic of China; 2Department of Oncology, The Second People’s Hospital of Kunshan, Kunshan, People’s Republic of China; 3Department of Oncology, Liyang People’s Hospital, Liyang, People’s Republic of China; 4Department of Oncology, Affiliated Hospital of Chinese Medicine of Changshu City, Nanjing University of Chinese Medicine, Changshu, People’s Republic of China; 5Nanosynthesis Unit, Nanome Consulting, Salem, Tamil Nadu, India*These authors contributed equally to this workCorrespondence: Lichun DengDepartment of Oncology, Affiliated Jiangyin Hospital of Medical College, Southeast University, No. 163 Shoushan Road, Jiangyin, 214400, Jiangsu Province, People’s Republic of ChinaTel +86 510 8061 5131Email 18921233265@163.comJoe Antony JacobNanosynthesis Unit, Nanome Consulting, No. 11, Kannankurichi Road, Salem, 636008, Tamil Nadu, IndiaTel +91-9443861940Email joeantonyjacob@gmail.comPurpose: Biomimetic approaches for the synthesis of silver nanoparticles (AgNPs) had created a substantial impression among the research community that focuses on nano-bio interactions. In this study, an eco-friendly method using Rhizophora apiculata aqueous leaf extract as a reductant-rich hydrosol was followed to synthesize AgNPs and test its cytotoxicity.Methods: To optimise the parameters for the synthesis of AgNPs, central composite design based on response surface methodology was used. The particles synthesized at a nano-scale were characterized in our previously published report. The present report further characterizes the nanoparticles by X-ray diffraction, SEM and TEM at varying sites and magnifications. The characterized AgNPs were tested for their cytotoxic effects on HEK-293 and HeLa cells.Results: The cytotoxicity on the cell lines was dose-dependent. At a concentration of 2.5 μL/mL of the AgNPs-containing hydrosol, 100% inhibition of HEK-293 cells and 75% inhibition of the HeLa cells were observed. The IC50 value for AgNPs on HEK-293 was 0.622 μL/mL (12.135 ng), whereas, for HeLa cells, it was 1.98 μL/mL (38.629 ng).Conclusion: The nanoparticles were three-fold toxic towards the HEK-293 cells in comparison to the HeLa cells. Therefore, the therapeutic index is low for R. apiculata derived AgNPs on HeLa cells when tested in comparison with the HEK-293 cells. The nanotoxicity profile of the synthesized AgNPs seems more prominent than the nanotherapeutic index. According to our knowledge, this is the first-ever report on the optimization of synthesis of AgNPs using response surface methodology and identifying the therapeutic index of mangrove leaf-derived AgNPs.Keywords: AgNPs, XRD, HEK-293, HeLa, nanotoxicity
format	article
author	Liu X Shan K Shao X Shi X He Y Liu Z Jacob JA Deng L
author_facet	Liu X Shan K Shao X Shi X He Y Liu Z Jacob JA Deng L
author_sort	Liu X
title	Nanotoxic Effects of Silver Nanoparticles on Normal HEK-293 Cells in Comparison to Cancerous HeLa Cell Line
title_short	Nanotoxic Effects of Silver Nanoparticles on Normal HEK-293 Cells in Comparison to Cancerous HeLa Cell Line
title_full	Nanotoxic Effects of Silver Nanoparticles on Normal HEK-293 Cells in Comparison to Cancerous HeLa Cell Line
title_fullStr	Nanotoxic Effects of Silver Nanoparticles on Normal HEK-293 Cells in Comparison to Cancerous HeLa Cell Line
title_full_unstemmed	Nanotoxic Effects of Silver Nanoparticles on Normal HEK-293 Cells in Comparison to Cancerous HeLa Cell Line
title_sort	nanotoxic effects of silver nanoparticles on normal hek-293 cells in comparison to cancerous hela cell line
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/a11dd5d5b2494ed68e028f89d827a82b
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Nanotoxic Effects of Silver Nanoparticles on Normal HEK-293 Cells in Comparison to Cancerous HeLa Cell Line

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