Colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms

Jeevan Thavanathan,1 Nay Ming Huang,1 Kwai Lin Thong2 1Low Dimension Material Research Center, Department of Physics, 2Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia Abstract: We have developed a colorimetric biosensor using a dual plat...

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Autores principales:	Thavanathan J, Huang NM, Thong KL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a11ddbc22c4347479f2e61df166bd401
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spelling	oai:doaj.org-article:a11ddbc22c4347479f2e61df166bd4012021-12-02T00:39:21ZColorimetric biosensing of targeted gene sequence using dual nanoparticle platforms1178-2013https://doaj.org/article/a11ddbc22c4347479f2e61df166bd4012015-04-01T00:00:00Zhttp://www.dovepress.com/colorimetric-biosensing-of-targeted-gene-sequence-using-dual-nanoparti-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Jeevan Thavanathan,1 Nay Ming Huang,1 Kwai Lin Thong2 1Low Dimension Material Research Center, Department of Physics, 2Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia Abstract: We have developed a colorimetric biosensor using a dual platform of gold nanoparticles and graphene oxide sheets for the detection of Salmonella enterica. The presence of the invA gene in S. enterica causes a change in color of the biosensor from its original pinkish-red to a light purplish solution. This occurs through the aggregation of the primary gold nanoparticles–conjugated DNA probe onto the surface of the secondary graphene oxide–conjugated DNA probe through DNA hybridization with the targeted DNA sequence. Spectrophotometry analysis showed a shift in wavelength from 525 nm to 600 nm with 1 µM of DNA target. Specificity testing revealed that the biosensor was able to detect various serovars of the S. enterica while no color change was observed with the other bacterial species. Sensitivity testing revealed the limit of detection was at 1 nM of DNA target. This proves the effectiveness of the biosensor in the detection of S. enterica through DNA hybridization. Keywords: biosensor, DNA hybridization, DNA probe, gold nanoparticles, graphene oxide, Salmonella entericaThavanathan JHuang NMThong KLDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 2711-2722 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Thavanathan J Huang NM Thong KL Colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms
description	Jeevan Thavanathan,1 Nay Ming Huang,1 Kwai Lin Thong2 1Low Dimension Material Research Center, Department of Physics, 2Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia Abstract: We have developed a colorimetric biosensor using a dual platform of gold nanoparticles and graphene oxide sheets for the detection of Salmonella enterica. The presence of the invA gene in S. enterica causes a change in color of the biosensor from its original pinkish-red to a light purplish solution. This occurs through the aggregation of the primary gold nanoparticles–conjugated DNA probe onto the surface of the secondary graphene oxide–conjugated DNA probe through DNA hybridization with the targeted DNA sequence. Spectrophotometry analysis showed a shift in wavelength from 525 nm to 600 nm with 1 µM of DNA target. Specificity testing revealed that the biosensor was able to detect various serovars of the S. enterica while no color change was observed with the other bacterial species. Sensitivity testing revealed the limit of detection was at 1 nM of DNA target. This proves the effectiveness of the biosensor in the detection of S. enterica through DNA hybridization. Keywords: biosensor, DNA hybridization, DNA probe, gold nanoparticles, graphene oxide, Salmonella enterica
format	article
author	Thavanathan J Huang NM Thong KL
author_facet	Thavanathan J Huang NM Thong KL
author_sort	Thavanathan J
title	Colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms
title_short	Colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms
title_full	Colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms
title_fullStr	Colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms
title_full_unstemmed	Colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms
title_sort	colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/a11ddbc22c4347479f2e61df166bd401
work_keys_str_mv	AT thavanathanj colorimetricbiosensingoftargetedgenesequenceusingdualnanoparticleplatforms AT huangnm colorimetricbiosensingoftargetedgenesequenceusingdualnanoparticleplatforms AT thongkl colorimetricbiosensingoftargetedgenesequenceusingdualnanoparticleplatforms
_version_	1718403572429750272

Colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms

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