Wound-healing effect of micronized sacchachitin (mSC) nanogel on corneal epithelium

Ray-Neng Chen,1,* Lin-Wen Lee,2,5,* Ling-Chun Chen,3 Hsiu-O Ho,3 Shiao-Chuan Lui,2 Ming-Thau Sheu,3,4 Ching-Hua Su2 1Department of Cosmetic Science and Management, Mackay Medicine, Nursing and Management College, Taipei, 2Department of Microbiology and Immunology, School of Medicine, College of Medi...

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Autores principales:	Chen RN, Lee LW, Chen LC, Ho HO, Lui SC, Sheu MT, Su CH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a126589f69874cfdb750fd2e7eb3331d
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spelling	oai:doaj.org-article:a126589f69874cfdb750fd2e7eb3331d2021-12-02T01:19:12ZWound-healing effect of micronized sacchachitin (mSC) nanogel on corneal epithelium1176-91141178-2013https://doaj.org/article/a126589f69874cfdb750fd2e7eb3331d2012-08-01T00:00:00Zhttp://www.dovepress.com/wound-healing-effect-of-micronized-sacchachitin-msc-nanogel-on-corneal-a10838https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Ray-Neng Chen,1,* Lin-Wen Lee,2,5,* Ling-Chun Chen,3 Hsiu-O Ho,3 Shiao-Chuan Lui,2 Ming-Thau Sheu,3,4 Ching-Hua Su2 1Department of Cosmetic Science and Management, Mackay Medicine, Nursing and Management College, Taipei, 2Department of Microbiology and Immunology, School of Medicine, College of Medicine, 3School of Pharmacy, College of Pharmacy, 4Clinical Research Center and Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei Medical University, 5Research Center for Biomedical Devices and Prototyping Production, Taipei Medical University, Taipei, Taiwan, Republic of China*These authors contributed equally to this workAbstract: The extraction residue of the Ganoderma fruiting body, named sacchachitin, has been demonstrated to have the potential to enhance cutaneous wound healing by inducing cell proliferation. In this study, a nanogel formed from micronized sacchachitin (mSC) was investigated for the potential treatment of superficial chemical corneal burns. Reportedly, mSC has been produced successfully and its chemical properties confirmed, and physical and rheological properties characterized. An in vitro cell proliferation study has revealed that at the concentrations of 200, 300, and 400 &micro;g/mL, mSC nanogel significantly increased Statens Seruminstitut rabbit corneal (SIRC) cell proliferation after 24 hours of incubation. In cell migration assay, migration of SIRC cell to wound closure was observed after 24 hours of incubation with the addition of 200 &micro;g/mL mSC of nanogel. In an animal study, acceleration of corneal wound healing was probably due to the inhibition of proteolysis. In conclusion, the findings of this study substantiate the potential application of sacchachitin in the form of mSC nanogel for the treatment of superficial corneal injuries.Keywords: corneal wound healing, sacchachitin, matrix metalloproteinaseChen RNLee LWChen LCHo HOLui SCSheu MTSu CHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 4697-4706 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Chen RN Lee LW Chen LC Ho HO Lui SC Sheu MT Su CH Wound-healing effect of micronized sacchachitin (mSC) nanogel on corneal epithelium
description	Ray-Neng Chen,1,* Lin-Wen Lee,2,5,* Ling-Chun Chen,3 Hsiu-O Ho,3 Shiao-Chuan Lui,2 Ming-Thau Sheu,3,4 Ching-Hua Su2 1Department of Cosmetic Science and Management, Mackay Medicine, Nursing and Management College, Taipei, 2Department of Microbiology and Immunology, School of Medicine, College of Medicine, 3School of Pharmacy, College of Pharmacy, 4Clinical Research Center and Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei Medical University, 5Research Center for Biomedical Devices and Prototyping Production, Taipei Medical University, Taipei, Taiwan, Republic of China*These authors contributed equally to this workAbstract: The extraction residue of the Ganoderma fruiting body, named sacchachitin, has been demonstrated to have the potential to enhance cutaneous wound healing by inducing cell proliferation. In this study, a nanogel formed from micronized sacchachitin (mSC) was investigated for the potential treatment of superficial chemical corneal burns. Reportedly, mSC has been produced successfully and its chemical properties confirmed, and physical and rheological properties characterized. An in vitro cell proliferation study has revealed that at the concentrations of 200, 300, and 400 &micro;g/mL, mSC nanogel significantly increased Statens Seruminstitut rabbit corneal (SIRC) cell proliferation after 24 hours of incubation. In cell migration assay, migration of SIRC cell to wound closure was observed after 24 hours of incubation with the addition of 200 &micro;g/mL mSC of nanogel. In an animal study, acceleration of corneal wound healing was probably due to the inhibition of proteolysis. In conclusion, the findings of this study substantiate the potential application of sacchachitin in the form of mSC nanogel for the treatment of superficial corneal injuries.Keywords: corneal wound healing, sacchachitin, matrix metalloproteinase
format	article
author	Chen RN Lee LW Chen LC Ho HO Lui SC Sheu MT Su CH
author_facet	Chen RN Lee LW Chen LC Ho HO Lui SC Sheu MT Su CH
author_sort	Chen RN
title	Wound-healing effect of micronized sacchachitin (mSC) nanogel on corneal epithelium
title_short	Wound-healing effect of micronized sacchachitin (mSC) nanogel on corneal epithelium
title_full	Wound-healing effect of micronized sacchachitin (mSC) nanogel on corneal epithelium
title_fullStr	Wound-healing effect of micronized sacchachitin (mSC) nanogel on corneal epithelium
title_full_unstemmed	Wound-healing effect of micronized sacchachitin (mSC) nanogel on corneal epithelium
title_sort	wound-healing effect of micronized sacchachitin (msc) nanogel on corneal epithelium
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/a126589f69874cfdb750fd2e7eb3331d
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Wound-healing effect of micronized sacchachitin (mSC) nanogel on corneal epithelium

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