Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesian archipelago based on morphological and inter-simple sequence repeats markers

Abstract. Makmur K, Chikmawati T, Sobir. 2020. Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesia based on morphological characters and ISSR markers. Biodiversitas 21: 5948-5953. Lemon basil (Ocimum × africanum Lour.) or kemangi is a highly beneficial plant, yet still under...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kurnia Makmur, TATIK CHIKMAWATI, SOBIR SOBIR
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	lemon basil, genetic diversity, issr, morphology, ocimum × africanum Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a129d500ce0942fa94114e550a7a3316
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a129d500ce0942fa94114e550a7a3316
record_format	dspace
spelling	oai:doaj.org-article:a129d500ce0942fa94114e550a7a33162021-11-22T00:52:00ZGenetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesian archipelago based on morphological and inter-simple sequence repeats markers1412-033X2085-472210.13057/biodiv/d211259https://doaj.org/article/a129d500ce0942fa94114e550a7a33162020-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6719https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Makmur K, Chikmawati T, Sobir. 2020. Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesia based on morphological characters and ISSR markers. Biodiversitas 21: 5948-5953. Lemon basil (Ocimum × africanum Lour.) or kemangi is a highly beneficial plant, yet still underutilized. Therefore, providing necessary information on genetic diversity of this species is essential for further utilization. The aim of this study was to elucidate genetic diversity of 33 accessions of O. × africanum collected from four Islands of Indonesia along with three accessions of Ocimum basilicum L. Morphological observation was conducted on 37 morphological characters following the International Union for the Protection of New Varieties of Plants descriptor guidelines; subsequently, 13 ISSR primers were employed in molecular analysis. Both morphological and molecular data were analyzed based on simple matching similarity index using UPGMA method. Morphologically, O. × africanum and O. basilicum were clearly separated at the similarity index of 0.52, and among two species were divided into two groups according to two either character. ISSR analysis using 13 ISSR primers produced 111 DNA bands, and 108 of them (97.29%) were polymorphic. Cluster analysis based on ISSR data could not explicitly separate O. × africanum and O. basilicum accessions. Besides, Ocimum accessions collected from the same area did not always cluster into one group.Kurnia MakmurTATIK CHIKMAWATISOBIR SOBIRMBI & UNS Soloarticlelemon basil, genetic diversity, issr, morphology, ocimum × africanumBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	lemon basil, genetic diversity, issr, morphology, ocimum × africanum Biology (General) QH301-705.5
spellingShingle	lemon basil, genetic diversity, issr, morphology, ocimum × africanum Biology (General) QH301-705.5 Kurnia Makmur TATIK CHIKMAWATI SOBIR SOBIR Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesian archipelago based on morphological and inter-simple sequence repeats markers
description	Abstract. Makmur K, Chikmawati T, Sobir. 2020. Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesia based on morphological characters and ISSR markers. Biodiversitas 21: 5948-5953. Lemon basil (Ocimum × africanum Lour.) or kemangi is a highly beneficial plant, yet still underutilized. Therefore, providing necessary information on genetic diversity of this species is essential for further utilization. The aim of this study was to elucidate genetic diversity of 33 accessions of O. × africanum collected from four Islands of Indonesia along with three accessions of Ocimum basilicum L. Morphological observation was conducted on 37 morphological characters following the International Union for the Protection of New Varieties of Plants descriptor guidelines; subsequently, 13 ISSR primers were employed in molecular analysis. Both morphological and molecular data were analyzed based on simple matching similarity index using UPGMA method. Morphologically, O. × africanum and O. basilicum were clearly separated at the similarity index of 0.52, and among two species were divided into two groups according to two either character. ISSR analysis using 13 ISSR primers produced 111 DNA bands, and 108 of them (97.29%) were polymorphic. Cluster analysis based on ISSR data could not explicitly separate O. × africanum and O. basilicum accessions. Besides, Ocimum accessions collected from the same area did not always cluster into one group.
format	article
author	Kurnia Makmur TATIK CHIKMAWATI SOBIR SOBIR
author_facet	Kurnia Makmur TATIK CHIKMAWATI SOBIR SOBIR
author_sort	Kurnia Makmur
title	Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesian archipelago based on morphological and inter-simple sequence repeats markers
title_short	Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesian archipelago based on morphological and inter-simple sequence repeats markers
title_full	Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesian archipelago based on morphological and inter-simple sequence repeats markers
title_fullStr	Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesian archipelago based on morphological and inter-simple sequence repeats markers
title_full_unstemmed	Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesian archipelago based on morphological and inter-simple sequence repeats markers
title_sort	genetic variability of lemon basil (ocimum × africanum lour.) from indonesian archipelago based on morphological and inter-simple sequence repeats markers
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/a129d500ce0942fa94114e550a7a3316
work_keys_str_mv	AT kurniamakmur geneticvariabilityoflemonbasilocimumafricanumlourfromindonesianarchipelagobasedonmorphologicalandintersimplesequencerepeatsmarkers AT tatikchikmawati geneticvariabilityoflemonbasilocimumafricanumlourfromindonesianarchipelagobasedonmorphologicalandintersimplesequencerepeatsmarkers AT sobirsobir geneticvariabilityoflemonbasilocimumafricanumlourfromindonesianarchipelagobasedonmorphologicalandintersimplesequencerepeatsmarkers
_version_	1718418476928860160

Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesian archipelago based on morphological and inter-simple sequence repeats markers

Ejemplares similares