Exploiting the Anti-Inflammatory Potential of White <i>Capsicum</i> Extract by the Nanoformulation in Phospholipid Vesicles

The peppers of the <i>Capsicum</i> species are exploited in many fields, as flavoring agents in food industry, or as decorative and therapeutic plants. Peppers show a diversified phytochemical content responsible for different biological activities. Synergic activity exerted by high leve...

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Autores principales:	Ilaria Pappalardo, Anna Santarsiero, Maria De Luca, Maria Assunta Acquavia, Simona Todisco, Carla Caddeo, Giuliana Bianco, Vittoria Infantino, Giuseppe Martelli, Antonio Vassallo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	white <i>Capsicum</i> extract phospholipid vesicles anti-inflammatory ROS NO cells Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/a12e1ef255f949dda185a9557945a91b
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spelling	oai:doaj.org-article:a12e1ef255f949dda185a9557945a91b2021-11-25T16:26:03ZExploiting the Anti-Inflammatory Potential of White <i>Capsicum</i> Extract by the Nanoformulation in Phospholipid Vesicles10.3390/antiox101116832076-3921https://doaj.org/article/a12e1ef255f949dda185a9557945a91b2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1683https://doaj.org/toc/2076-3921The peppers of the <i>Capsicum</i> species are exploited in many fields, as flavoring agents in food industry, or as decorative and therapeutic plants. Peppers show a diversified phytochemical content responsible for different biological activities. Synergic activity exerted by high levels of antioxidant compounds is responsible for their important anti-inflammatory property. A methanolic extract was obtained from a new pepper genotype and tested for anti-inflammatory activity. The extract was incorporated into phospholipid vesicles to increase the bioavailability of its bioactive components. Two types of phospholipid vesicles were produced, conventional liposomes and Penetration Enhancer containing Vesicles (PEVs). They were tested in human monoblastic leukemia U937 cell line, showing no cytotoxic effect. The intracellular reactive oxygen species (ROS) and nitric oxide (NO) levels were measured to value the in vitro efficacy of the vesicles in regulating inflammatory responses. Liposomal incorporation significantly reduced ROS levels in extract-treated LPS-activated cells. Furthermore, LC-MS/MS analyses demonstrated that liposomes facilitated the transport of the extract components across the cell membrane and their accumulation into the cytoplasm.Ilaria PappalardoAnna SantarsieroMaria De LucaMaria Assunta AcquaviaSimona TodiscoCarla CaddeoGiuliana BiancoVittoria InfantinoGiuseppe MartelliAntonio VassalloMDPI AGarticlewhite <i>Capsicum</i> extractphospholipid vesiclesanti-inflammatoryROSNOcellsTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1683, p 1683 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	white <i>Capsicum</i> extract phospholipid vesicles anti-inflammatory ROS NO cells Therapeutics. Pharmacology RM1-950
spellingShingle	white <i>Capsicum</i> extract phospholipid vesicles anti-inflammatory ROS NO cells Therapeutics. Pharmacology RM1-950 Ilaria Pappalardo Anna Santarsiero Maria De Luca Maria Assunta Acquavia Simona Todisco Carla Caddeo Giuliana Bianco Vittoria Infantino Giuseppe Martelli Antonio Vassallo Exploiting the Anti-Inflammatory Potential of White <i>Capsicum</i> Extract by the Nanoformulation in Phospholipid Vesicles
description	The peppers of the <i>Capsicum</i> species are exploited in many fields, as flavoring agents in food industry, or as decorative and therapeutic plants. Peppers show a diversified phytochemical content responsible for different biological activities. Synergic activity exerted by high levels of antioxidant compounds is responsible for their important anti-inflammatory property. A methanolic extract was obtained from a new pepper genotype and tested for anti-inflammatory activity. The extract was incorporated into phospholipid vesicles to increase the bioavailability of its bioactive components. Two types of phospholipid vesicles were produced, conventional liposomes and Penetration Enhancer containing Vesicles (PEVs). They were tested in human monoblastic leukemia U937 cell line, showing no cytotoxic effect. The intracellular reactive oxygen species (ROS) and nitric oxide (NO) levels were measured to value the in vitro efficacy of the vesicles in regulating inflammatory responses. Liposomal incorporation significantly reduced ROS levels in extract-treated LPS-activated cells. Furthermore, LC-MS/MS analyses demonstrated that liposomes facilitated the transport of the extract components across the cell membrane and their accumulation into the cytoplasm.
format	article
author	Ilaria Pappalardo Anna Santarsiero Maria De Luca Maria Assunta Acquavia Simona Todisco Carla Caddeo Giuliana Bianco Vittoria Infantino Giuseppe Martelli Antonio Vassallo
author_facet	Ilaria Pappalardo Anna Santarsiero Maria De Luca Maria Assunta Acquavia Simona Todisco Carla Caddeo Giuliana Bianco Vittoria Infantino Giuseppe Martelli Antonio Vassallo
author_sort	Ilaria Pappalardo
title	Exploiting the Anti-Inflammatory Potential of White <i>Capsicum</i> Extract by the Nanoformulation in Phospholipid Vesicles
title_short	Exploiting the Anti-Inflammatory Potential of White <i>Capsicum</i> Extract by the Nanoformulation in Phospholipid Vesicles
title_full	Exploiting the Anti-Inflammatory Potential of White <i>Capsicum</i> Extract by the Nanoformulation in Phospholipid Vesicles
title_fullStr	Exploiting the Anti-Inflammatory Potential of White <i>Capsicum</i> Extract by the Nanoformulation in Phospholipid Vesicles
title_full_unstemmed	Exploiting the Anti-Inflammatory Potential of White <i>Capsicum</i> Extract by the Nanoformulation in Phospholipid Vesicles
title_sort	exploiting the anti-inflammatory potential of white <i>capsicum</i> extract by the nanoformulation in phospholipid vesicles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a12e1ef255f949dda185a9557945a91b
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Exploiting the Anti-Inflammatory Potential of White <i>Capsicum</i> Extract by the Nanoformulation in Phospholipid Vesicles

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