Proteome-wide profiling and mapping of post translational modifications in human hearts

Abstract Post translational modifications (PTMs) are covalent modifications of proteins that can range from small chemical modifications to addition of entire proteins. PTMs contribute to regulation of protein function and thereby greatly increase the functional diversity of the proteome. In the hea...

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Autores principales:	Navratan Bagwan, Henrik H. El Ali, Alicia Lundby
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a132d662f1604d1b8f12973716e6e4c7
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spelling	oai:doaj.org-article:a132d662f1604d1b8f12973716e6e4c72021-12-02T10:48:03ZProteome-wide profiling and mapping of post translational modifications in human hearts10.1038/s41598-021-81986-y2045-2322https://doaj.org/article/a132d662f1604d1b8f12973716e6e4c72021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81986-yhttps://doaj.org/toc/2045-2322Abstract Post translational modifications (PTMs) are covalent modifications of proteins that can range from small chemical modifications to addition of entire proteins. PTMs contribute to regulation of protein function and thereby greatly increase the functional diversity of the proteome. In the heart, a few well-studied PTMs, such as phosphorylation and glycosylation, are known to play essential roles for cardiac function. Yet, only a fraction of the ~ 300 known PTMs have been studied in a cardiac context. Here we investigated the proteome-wide map of PTMs present in human hearts by utilizing high-resolution mass spectrometry measurements and a suite of PTM identification algorithms. Our approach led to identification of more than 150 different PTMs across three of the chambers in human hearts. This finding underscores that decoration of cardiac proteins by PTMs is much more diverse than hitherto appreciated and provides insights in cardiac protein PTMs not yet studied. The results presented serve as a catalogue of which PTMs are present in human hearts and outlines the particular protein and the specific amino acid modified, and thereby provides a detail-rich resource for exploring protein modifications in human hearts beyond the most studied PTMs.Navratan BagwanHenrik H. El AliAlicia LundbyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Navratan Bagwan Henrik H. El Ali Alicia Lundby Proteome-wide profiling and mapping of post translational modifications in human hearts
description	Abstract Post translational modifications (PTMs) are covalent modifications of proteins that can range from small chemical modifications to addition of entire proteins. PTMs contribute to regulation of protein function and thereby greatly increase the functional diversity of the proteome. In the heart, a few well-studied PTMs, such as phosphorylation and glycosylation, are known to play essential roles for cardiac function. Yet, only a fraction of the ~ 300 known PTMs have been studied in a cardiac context. Here we investigated the proteome-wide map of PTMs present in human hearts by utilizing high-resolution mass spectrometry measurements and a suite of PTM identification algorithms. Our approach led to identification of more than 150 different PTMs across three of the chambers in human hearts. This finding underscores that decoration of cardiac proteins by PTMs is much more diverse than hitherto appreciated and provides insights in cardiac protein PTMs not yet studied. The results presented serve as a catalogue of which PTMs are present in human hearts and outlines the particular protein and the specific amino acid modified, and thereby provides a detail-rich resource for exploring protein modifications in human hearts beyond the most studied PTMs.
format	article
author	Navratan Bagwan Henrik H. El Ali Alicia Lundby
author_facet	Navratan Bagwan Henrik H. El Ali Alicia Lundby
author_sort	Navratan Bagwan
title	Proteome-wide profiling and mapping of post translational modifications in human hearts
title_short	Proteome-wide profiling and mapping of post translational modifications in human hearts
title_full	Proteome-wide profiling and mapping of post translational modifications in human hearts
title_fullStr	Proteome-wide profiling and mapping of post translational modifications in human hearts
title_full_unstemmed	Proteome-wide profiling and mapping of post translational modifications in human hearts
title_sort	proteome-wide profiling and mapping of post translational modifications in human hearts
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a132d662f1604d1b8f12973716e6e4c7
work_keys_str_mv	AT navratanbagwan proteomewideprofilingandmappingofposttranslationalmodificationsinhumanhearts AT henrikhelali proteomewideprofilingandmappingofposttranslationalmodificationsinhumanhearts AT alicialundby proteomewideprofilingandmappingofposttranslationalmodificationsinhumanhearts
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Proteome-wide profiling and mapping of post translational modifications in human hearts

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