Physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice.
We evaluated whether the water dispersibility of lactic acid bacteria (Enterococcus faecalis KH2) affects their efficacy. When cultured lactic acid bacteria are washed, heat-killed, and powdered, adhesion occurs between results in aggregation (non-treated lactic acid bacteria, n-LAB). However, dispe...
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2021
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oai:doaj.org-article:a134502f5f984f3a95125b3834faee862021-11-25T06:19:08ZPhysical properties of lactic acid bacteria influence the level of protection against influenza infection in mice.1932-620310.1371/journal.pone.0251784https://doaj.org/article/a134502f5f984f3a95125b3834faee862021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251784https://doaj.org/toc/1932-6203We evaluated whether the water dispersibility of lactic acid bacteria (Enterococcus faecalis KH2) affects their efficacy. When cultured lactic acid bacteria are washed, heat-killed, and powdered, adhesion occurs between results in aggregation (non-treated lactic acid bacteria, n-LAB). However, dispersed lactic acid bacteria (d-LAB) with a lower number of aggregates can be prepared by treating them with a high-pressure homogenizer and adding an excipient during powdering. Mice were administered n-LAB or d-LAB Peyer's patches in the small intestine were observed. Following n-LAB administration, a high amount of aggregated bacteria drifting in the intestinal mucosa was observed; meanwhile, d-LAB reached the Peyer's patches and was absorbed into them. Evaluation in a mouse influenza virus infection model showed that d-LAB was more effective than n-LAB in the influenza yield of bronchoalveolar lavage fluids on day 3 post-infection and neutralizing antibody titers of sera and influenza virus-specific immunoglobulin A in the feces on day 14 post-infection. Therefore, the physical properties of lactic acid bacteria affect their efficacy; controlling their water dispersibility can improve their effectiveness.Takumi WatanabeKyoko HayashiIsao TakahashiMakoto OhwakiTatsuhiko KanToshio KawaharaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251784 (2021) |
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Medicine R Science Q Takumi Watanabe Kyoko Hayashi Isao Takahashi Makoto Ohwaki Tatsuhiko Kan Toshio Kawahara Physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice. |
description |
We evaluated whether the water dispersibility of lactic acid bacteria (Enterococcus faecalis KH2) affects their efficacy. When cultured lactic acid bacteria are washed, heat-killed, and powdered, adhesion occurs between results in aggregation (non-treated lactic acid bacteria, n-LAB). However, dispersed lactic acid bacteria (d-LAB) with a lower number of aggregates can be prepared by treating them with a high-pressure homogenizer and adding an excipient during powdering. Mice were administered n-LAB or d-LAB Peyer's patches in the small intestine were observed. Following n-LAB administration, a high amount of aggregated bacteria drifting in the intestinal mucosa was observed; meanwhile, d-LAB reached the Peyer's patches and was absorbed into them. Evaluation in a mouse influenza virus infection model showed that d-LAB was more effective than n-LAB in the influenza yield of bronchoalveolar lavage fluids on day 3 post-infection and neutralizing antibody titers of sera and influenza virus-specific immunoglobulin A in the feces on day 14 post-infection. Therefore, the physical properties of lactic acid bacteria affect their efficacy; controlling their water dispersibility can improve their effectiveness. |
format |
article |
author |
Takumi Watanabe Kyoko Hayashi Isao Takahashi Makoto Ohwaki Tatsuhiko Kan Toshio Kawahara |
author_facet |
Takumi Watanabe Kyoko Hayashi Isao Takahashi Makoto Ohwaki Tatsuhiko Kan Toshio Kawahara |
author_sort |
Takumi Watanabe |
title |
Physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice. |
title_short |
Physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice. |
title_full |
Physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice. |
title_fullStr |
Physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice. |
title_full_unstemmed |
Physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice. |
title_sort |
physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doaj.org/article/a134502f5f984f3a95125b3834faee86 |
work_keys_str_mv |
AT takumiwatanabe physicalpropertiesoflacticacidbacteriainfluencethelevelofprotectionagainstinfluenzainfectioninmice AT kyokohayashi physicalpropertiesoflacticacidbacteriainfluencethelevelofprotectionagainstinfluenzainfectioninmice AT isaotakahashi physicalpropertiesoflacticacidbacteriainfluencethelevelofprotectionagainstinfluenzainfectioninmice AT makotoohwaki physicalpropertiesoflacticacidbacteriainfluencethelevelofprotectionagainstinfluenzainfectioninmice AT tatsuhikokan physicalpropertiesoflacticacidbacteriainfluencethelevelofprotectionagainstinfluenzainfectioninmice AT toshiokawahara physicalpropertiesoflacticacidbacteriainfluencethelevelofprotectionagainstinfluenzainfectioninmice |
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1718413941369995264 |