Physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice.

We evaluated whether the water dispersibility of lactic acid bacteria (Enterococcus faecalis KH2) affects their efficacy. When cultured lactic acid bacteria are washed, heat-killed, and powdered, adhesion occurs between results in aggregation (non-treated lactic acid bacteria, n-LAB). However, dispe...

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Auteurs principaux:	Takumi Watanabe, Kyoko Hayashi, Isao Takahashi, Makoto Ohwaki, Tatsuhiko Kan, Toshio Kawahara
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2021
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/a134502f5f984f3a95125b3834faee86
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spelling	oai:doaj.org-article:a134502f5f984f3a95125b3834faee862021-11-25T06:19:08ZPhysical properties of lactic acid bacteria influence the level of protection against influenza infection in mice.1932-620310.1371/journal.pone.0251784https://doaj.org/article/a134502f5f984f3a95125b3834faee862021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251784https://doaj.org/toc/1932-6203We evaluated whether the water dispersibility of lactic acid bacteria (Enterococcus faecalis KH2) affects their efficacy. When cultured lactic acid bacteria are washed, heat-killed, and powdered, adhesion occurs between results in aggregation (non-treated lactic acid bacteria, n-LAB). However, dispersed lactic acid bacteria (d-LAB) with a lower number of aggregates can be prepared by treating them with a high-pressure homogenizer and adding an excipient during powdering. Mice were administered n-LAB or d-LAB Peyer's patches in the small intestine were observed. Following n-LAB administration, a high amount of aggregated bacteria drifting in the intestinal mucosa was observed; meanwhile, d-LAB reached the Peyer's patches and was absorbed into them. Evaluation in a mouse influenza virus infection model showed that d-LAB was more effective than n-LAB in the influenza yield of bronchoalveolar lavage fluids on day 3 post-infection and neutralizing antibody titers of sera and influenza virus-specific immunoglobulin A in the feces on day 14 post-infection. Therefore, the physical properties of lactic acid bacteria affect their efficacy; controlling their water dispersibility can improve their effectiveness.Takumi WatanabeKyoko HayashiIsao TakahashiMakoto OhwakiTatsuhiko KanToshio KawaharaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251784 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takumi Watanabe Kyoko Hayashi Isao Takahashi Makoto Ohwaki Tatsuhiko Kan Toshio Kawahara Physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice.
description	We evaluated whether the water dispersibility of lactic acid bacteria (Enterococcus faecalis KH2) affects their efficacy. When cultured lactic acid bacteria are washed, heat-killed, and powdered, adhesion occurs between results in aggregation (non-treated lactic acid bacteria, n-LAB). However, dispersed lactic acid bacteria (d-LAB) with a lower number of aggregates can be prepared by treating them with a high-pressure homogenizer and adding an excipient during powdering. Mice were administered n-LAB or d-LAB Peyer's patches in the small intestine were observed. Following n-LAB administration, a high amount of aggregated bacteria drifting in the intestinal mucosa was observed; meanwhile, d-LAB reached the Peyer's patches and was absorbed into them. Evaluation in a mouse influenza virus infection model showed that d-LAB was more effective than n-LAB in the influenza yield of bronchoalveolar lavage fluids on day 3 post-infection and neutralizing antibody titers of sera and influenza virus-specific immunoglobulin A in the feces on day 14 post-infection. Therefore, the physical properties of lactic acid bacteria affect their efficacy; controlling their water dispersibility can improve their effectiveness.
format	article
author	Takumi Watanabe Kyoko Hayashi Isao Takahashi Makoto Ohwaki Tatsuhiko Kan Toshio Kawahara
author_facet	Takumi Watanabe Kyoko Hayashi Isao Takahashi Makoto Ohwaki Tatsuhiko Kan Toshio Kawahara
author_sort	Takumi Watanabe
title	Physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice.
title_short	Physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice.
title_full	Physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice.
title_fullStr	Physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice.
title_full_unstemmed	Physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice.
title_sort	physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a134502f5f984f3a95125b3834faee86
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Physical properties of lactic acid bacteria influence the level of protection against influenza infection in mice.

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