Identification of TRPV4 as a novel target in invasiveness of colorectal cancer

Abstract Background Emerging evidence has indicated the critical role of TRPV4 in diverse human cancers. However, the underlying molecular mechanism of TRPV4 in colon cancer invasiveness is still unknown. Methods Immunohistochemistry staining was used to analyze the expression of TRPV4 and ZEB1 in c...

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Autores principales:	Peng Zhang, Jian Xu, Hua Zhang, Xiao-Yu Liu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Colon cancer TRPV4 Invasiveness ZEB1 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/a13d0dccf86b4faeb0d75f172fdaf56e
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spelling	oai:doaj.org-article:a13d0dccf86b4faeb0d75f172fdaf56e2021-11-28T12:27:33ZIdentification of TRPV4 as a novel target in invasiveness of colorectal cancer10.1186/s12885-021-08970-71471-2407https://doaj.org/article/a13d0dccf86b4faeb0d75f172fdaf56e2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12885-021-08970-7https://doaj.org/toc/1471-2407Abstract Background Emerging evidence has indicated the critical role of TRPV4 in diverse human cancers. However, the underlying molecular mechanism of TRPV4 in colon cancer invasiveness is still unknown. Methods Immunohistochemistry staining was used to analyze the expression of TRPV4 and ZEB1 in clinical tissues; Wound healing and transwell assays were applied to determine the cell invasiveness; Western blot was used to explore the relation between TRPV4 and ZEB1. Results Colon cancer cells were transfected with siRNA against TRPV4 or HC067047 (a selective TRPV4 antagonist), TRPV4 full-length plasmid or siRNA against ZEB1, or both, in order to measure cell migration and invasion. And we found that TRPV4 silencing or inhibition exhibited an inhibitory role in colon cancer cell migration and invasion, coupled with compromised EMT process, and suppressed AKT activity. TRPV4 stimulated expression of ZEB1 and consequently contributed to EMT process and invasiveness. It was also revealed that overexpression of TRPV4 and ZEB1 in clinical patients with local metastasis, and positive correlation between TRPV4 and ZEB1. Conclusions Our results uncovered the role of TRPV4 in tumor metastasis and highlighted the potential mechanism of TRPV4-ZEB1 axis in indicating EMT.Peng ZhangJian XuHua ZhangXiao-Yu LiuBMCarticleColon cancerTRPV4InvasivenessZEB1Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBMC Cancer, Vol 21, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Colon cancer TRPV4 Invasiveness ZEB1 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Colon cancer TRPV4 Invasiveness ZEB1 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Peng Zhang Jian Xu Hua Zhang Xiao-Yu Liu Identification of TRPV4 as a novel target in invasiveness of colorectal cancer
description	Abstract Background Emerging evidence has indicated the critical role of TRPV4 in diverse human cancers. However, the underlying molecular mechanism of TRPV4 in colon cancer invasiveness is still unknown. Methods Immunohistochemistry staining was used to analyze the expression of TRPV4 and ZEB1 in clinical tissues; Wound healing and transwell assays were applied to determine the cell invasiveness; Western blot was used to explore the relation between TRPV4 and ZEB1. Results Colon cancer cells were transfected with siRNA against TRPV4 or HC067047 (a selective TRPV4 antagonist), TRPV4 full-length plasmid or siRNA against ZEB1, or both, in order to measure cell migration and invasion. And we found that TRPV4 silencing or inhibition exhibited an inhibitory role in colon cancer cell migration and invasion, coupled with compromised EMT process, and suppressed AKT activity. TRPV4 stimulated expression of ZEB1 and consequently contributed to EMT process and invasiveness. It was also revealed that overexpression of TRPV4 and ZEB1 in clinical patients with local metastasis, and positive correlation between TRPV4 and ZEB1. Conclusions Our results uncovered the role of TRPV4 in tumor metastasis and highlighted the potential mechanism of TRPV4-ZEB1 axis in indicating EMT.
format	article
author	Peng Zhang Jian Xu Hua Zhang Xiao-Yu Liu
author_facet	Peng Zhang Jian Xu Hua Zhang Xiao-Yu Liu
author_sort	Peng Zhang
title	Identification of TRPV4 as a novel target in invasiveness of colorectal cancer
title_short	Identification of TRPV4 as a novel target in invasiveness of colorectal cancer
title_full	Identification of TRPV4 as a novel target in invasiveness of colorectal cancer
title_fullStr	Identification of TRPV4 as a novel target in invasiveness of colorectal cancer
title_full_unstemmed	Identification of TRPV4 as a novel target in invasiveness of colorectal cancer
title_sort	identification of trpv4 as a novel target in invasiveness of colorectal cancer
publisher	BMC
publishDate	2021
url	https://doaj.org/article/a13d0dccf86b4faeb0d75f172fdaf56e
work_keys_str_mv	AT pengzhang identificationoftrpv4asanoveltargetininvasivenessofcolorectalcancer AT jianxu identificationoftrpv4asanoveltargetininvasivenessofcolorectalcancer AT huazhang identificationoftrpv4asanoveltargetininvasivenessofcolorectalcancer AT xiaoyuliu identificationoftrpv4asanoveltargetininvasivenessofcolorectalcancer
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Identification of TRPV4 as a novel target in invasiveness of colorectal cancer

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