Prion Infectivity and PrP<sup>BSE</sup> in the Peripheral and Central Nervous System of Cattle 8 Months Post Oral BSE Challenge

After oral exposure of cattle with classical bovine spongiform encephalopathy (C-BSE), the infectious agent ascends from the gut to the central nervous system (CNS) primarily via the autonomic nervous system. However, the timeline of this progression has thus far remained widely undetermined. Previo...

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Autores principales:	Ivett Ackermann, Reiner Ulrich, Kerstin Tauscher, Olanrewaju I. Fatola, Markus Keller, James C. Shawulu, Mark Arnold, Stefanie Czub, Martin H. Groschup, Anne Balkema-Buschmann
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	prion protein BSE infectivity PrP<sup>BSE</sup> cattle peripheral and central nervous system Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/a13d8f1186a444bca4f51ad6092fc8d2
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spelling	oai:doaj.org-article:a13d8f1186a444bca4f51ad6092fc8d22021-11-11T16:48:03ZPrion Infectivity and PrP<sup>BSE</sup> in the Peripheral and Central Nervous System of Cattle 8 Months Post Oral BSE Challenge10.3390/ijms2221113101422-00671661-6596https://doaj.org/article/a13d8f1186a444bca4f51ad6092fc8d22021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11310https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067After oral exposure of cattle with classical bovine spongiform encephalopathy (C-BSE), the infectious agent ascends from the gut to the central nervous system (CNS) primarily via the autonomic nervous system. However, the timeline of this progression has thus far remained widely undetermined. Previous studies were focused on later time points after oral exposure of animals that were already 4 to 6 months old when challenged. In contrast, in this present study, we have orally inoculated 4 to 6 weeks old unweaned calves with high doses of BSE to identify any possible BSE infectivity and/or PrP<sup>BSE</sup> in peripheral nervous tissues during the first eight months post-inoculation (mpi). For the detection of BSE infectivity, we used a bovine PrP transgenic mouse bioassay, while PrP<sup>BSE</sup> depositions were analyzed by immunohistochemistry (IHC) and by protein misfolding cyclic amplification (PMCA). We were able to show that as early as 8 mpi the thoracic spinal cord as well as the parasympathetic nodal ganglion of these animals contained PrP<sup>BSE</sup> and BSE infectivity. This shows that the centripetal prion spread starts early after challenge at least in this age group, which represents an essential piece of information for the risk assessments for food, feed, and pharmaceutical products produced from young calves.Ivett AckermannReiner UlrichKerstin TauscherOlanrewaju I. FatolaMarkus KellerJames C. ShawuluMark ArnoldStefanie CzubMartin H. GroschupAnne Balkema-BuschmannMDPI AGarticleprion proteinBSEinfectivityPrP<sup>BSE</sup>cattleperipheral and central nervous systemBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11310, p 11310 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	prion protein BSE infectivity PrP<sup>BSE</sup> cattle peripheral and central nervous system Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	prion protein BSE infectivity PrP<sup>BSE</sup> cattle peripheral and central nervous system Biology (General) QH301-705.5 Chemistry QD1-999 Ivett Ackermann Reiner Ulrich Kerstin Tauscher Olanrewaju I. Fatola Markus Keller James C. Shawulu Mark Arnold Stefanie Czub Martin H. Groschup Anne Balkema-Buschmann Prion Infectivity and PrP<sup>BSE</sup> in the Peripheral and Central Nervous System of Cattle 8 Months Post Oral BSE Challenge
description	After oral exposure of cattle with classical bovine spongiform encephalopathy (C-BSE), the infectious agent ascends from the gut to the central nervous system (CNS) primarily via the autonomic nervous system. However, the timeline of this progression has thus far remained widely undetermined. Previous studies were focused on later time points after oral exposure of animals that were already 4 to 6 months old when challenged. In contrast, in this present study, we have orally inoculated 4 to 6 weeks old unweaned calves with high doses of BSE to identify any possible BSE infectivity and/or PrP<sup>BSE</sup> in peripheral nervous tissues during the first eight months post-inoculation (mpi). For the detection of BSE infectivity, we used a bovine PrP transgenic mouse bioassay, while PrP<sup>BSE</sup> depositions were analyzed by immunohistochemistry (IHC) and by protein misfolding cyclic amplification (PMCA). We were able to show that as early as 8 mpi the thoracic spinal cord as well as the parasympathetic nodal ganglion of these animals contained PrP<sup>BSE</sup> and BSE infectivity. This shows that the centripetal prion spread starts early after challenge at least in this age group, which represents an essential piece of information for the risk assessments for food, feed, and pharmaceutical products produced from young calves.
format	article
author	Ivett Ackermann Reiner Ulrich Kerstin Tauscher Olanrewaju I. Fatola Markus Keller James C. Shawulu Mark Arnold Stefanie Czub Martin H. Groschup Anne Balkema-Buschmann
author_facet	Ivett Ackermann Reiner Ulrich Kerstin Tauscher Olanrewaju I. Fatola Markus Keller James C. Shawulu Mark Arnold Stefanie Czub Martin H. Groschup Anne Balkema-Buschmann
author_sort	Ivett Ackermann
title	Prion Infectivity and PrP<sup>BSE</sup> in the Peripheral and Central Nervous System of Cattle 8 Months Post Oral BSE Challenge
title_short	Prion Infectivity and PrP<sup>BSE</sup> in the Peripheral and Central Nervous System of Cattle 8 Months Post Oral BSE Challenge
title_full	Prion Infectivity and PrP<sup>BSE</sup> in the Peripheral and Central Nervous System of Cattle 8 Months Post Oral BSE Challenge
title_fullStr	Prion Infectivity and PrP<sup>BSE</sup> in the Peripheral and Central Nervous System of Cattle 8 Months Post Oral BSE Challenge
title_full_unstemmed	Prion Infectivity and PrP<sup>BSE</sup> in the Peripheral and Central Nervous System of Cattle 8 Months Post Oral BSE Challenge
title_sort	prion infectivity and prp<sup>bse</sup> in the peripheral and central nervous system of cattle 8 months post oral bse challenge
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a13d8f1186a444bca4f51ad6092fc8d2
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Prion Infectivity and PrP<sup>BSE</sup> in the Peripheral and Central Nervous System of Cattle 8 Months Post Oral BSE Challenge

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