Systematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells

Abstract Urine pH has been thought to be an important factor that can modulate kidney stone formation. Nevertheless, there was no systematic evaluation of such pH effect. Our present study thus addressed effects of differential urine pH (4.0–8.0) on calcium oxalate (CaOx) crystallization, crystal-ce...

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Autores principales:	Juthatip Manissorn, Kedsarin Fong-ngern, Paleerath Peerapen, Visith Thongboonkerd
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a140fb2f93fa475690c8c0591a1c41f1
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spelling	oai:doaj.org-article:a140fb2f93fa475690c8c0591a1c41f12021-12-02T16:06:57ZSystematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells10.1038/s41598-017-01953-42045-2322https://doaj.org/article/a140fb2f93fa475690c8c0591a1c41f12017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01953-4https://doaj.org/toc/2045-2322Abstract Urine pH has been thought to be an important factor that can modulate kidney stone formation. Nevertheless, there was no systematic evaluation of such pH effect. Our present study thus addressed effects of differential urine pH (4.0–8.0) on calcium oxalate (CaOx) crystallization, crystal-cell adhesion, crystal internalization into renal tubular cells, and binding of apical membrane proteins to the crystals. Microscopic examination revealed that CaOx monohydrate (COM), the pathogenic form, was crystallized with greatest size, number and total mass at pH 4.0 and least crystallized at pH 8.0, whereas COD was crystallized with the vice versa order. Fourier-transform infrared (FT-IR) spectroscopy confirmed such morphological study. Crystal-cell adhesion assay showed the greatest degree of crystal-cell adhesion at the most acidic pH and least at the most basic pH. Crystal internalization assay using fluorescein isothiocyanate (FITC)-labelled crystals and flow cytometry demonstrated that crystal internalization into renal tubular cells was maximal at the neutral pH (7.0). Finally, there were no significant differences in binding capacity of the crystals to apical membrane proteins at different pH. We concluded that the acidic urine pH may promote CaOx kidney stone formation, whereas the basic urine pH (i.e. by alkalinization) may help to prevent CaOx kidney stone disease.Juthatip ManissornKedsarin Fong-ngernPaleerath PeerapenVisith ThongboonkerdNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juthatip Manissorn Kedsarin Fong-ngern Paleerath Peerapen Visith Thongboonkerd Systematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells
description	Abstract Urine pH has been thought to be an important factor that can modulate kidney stone formation. Nevertheless, there was no systematic evaluation of such pH effect. Our present study thus addressed effects of differential urine pH (4.0–8.0) on calcium oxalate (CaOx) crystallization, crystal-cell adhesion, crystal internalization into renal tubular cells, and binding of apical membrane proteins to the crystals. Microscopic examination revealed that CaOx monohydrate (COM), the pathogenic form, was crystallized with greatest size, number and total mass at pH 4.0 and least crystallized at pH 8.0, whereas COD was crystallized with the vice versa order. Fourier-transform infrared (FT-IR) spectroscopy confirmed such morphological study. Crystal-cell adhesion assay showed the greatest degree of crystal-cell adhesion at the most acidic pH and least at the most basic pH. Crystal internalization assay using fluorescein isothiocyanate (FITC)-labelled crystals and flow cytometry demonstrated that crystal internalization into renal tubular cells was maximal at the neutral pH (7.0). Finally, there were no significant differences in binding capacity of the crystals to apical membrane proteins at different pH. We concluded that the acidic urine pH may promote CaOx kidney stone formation, whereas the basic urine pH (i.e. by alkalinization) may help to prevent CaOx kidney stone disease.
format	article
author	Juthatip Manissorn Kedsarin Fong-ngern Paleerath Peerapen Visith Thongboonkerd
author_facet	Juthatip Manissorn Kedsarin Fong-ngern Paleerath Peerapen Visith Thongboonkerd
author_sort	Juthatip Manissorn
title	Systematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells
title_short	Systematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells
title_full	Systematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells
title_fullStr	Systematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells
title_full_unstemmed	Systematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells
title_sort	systematic evaluation for effects of urine ph on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a140fb2f93fa475690c8c0591a1c41f1
work_keys_str_mv	AT juthatipmanissorn systematicevaluationforeffectsofurinephoncalciumoxalatecrystallizationcrystalcelladhesionandinternalizationintorenaltubularcells AT kedsarinfongngern systematicevaluationforeffectsofurinephoncalciumoxalatecrystallizationcrystalcelladhesionandinternalizationintorenaltubularcells AT paleerathpeerapen systematicevaluationforeffectsofurinephoncalciumoxalatecrystallizationcrystalcelladhesionandinternalizationintorenaltubularcells AT visiththongboonkerd systematicevaluationforeffectsofurinephoncalciumoxalatecrystallizationcrystalcelladhesionandinternalizationintorenaltubularcells
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Systematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells

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