Circulating microRNA profiles of Hendra virus infection in horses

Abstract Hendra virus (HeV) is an emerging zoonotic pathogen harbored by Australian mainland flying foxes. HeV infection can cause lethal disease in humans and horses, and to date all cases of human HeV disease have resulted from contact with infected horses. Currently, diagnosis of acute HeV infect...

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Autores principales:	Christopher Cowled, Chwan-Hong Foo, Celine Deffrasnes, Christina L. Rootes, David T. Williams, Deborah Middleton, Lin-Fa Wang, Andrew G. D. Bean, Cameron R. Stewart
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a14697fa40a64d4786ec438ad18eadcf
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spelling	oai:doaj.org-article:a14697fa40a64d4786ec438ad18eadcf2021-12-02T15:05:14ZCirculating microRNA profiles of Hendra virus infection in horses10.1038/s41598-017-06939-w2045-2322https://doaj.org/article/a14697fa40a64d4786ec438ad18eadcf2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06939-whttps://doaj.org/toc/2045-2322Abstract Hendra virus (HeV) is an emerging zoonotic pathogen harbored by Australian mainland flying foxes. HeV infection can cause lethal disease in humans and horses, and to date all cases of human HeV disease have resulted from contact with infected horses. Currently, diagnosis of acute HeV infections in horses relies on the productive phase of infection when virus shedding may occur. An assay that identifies infected horses during the preclinical phase of infection would reduce the risk of zoonotic viral transmission during management of HeV outbreaks. Having previously shown that the host microRNA (miR)-146a is upregulated in the blood of HeV-infected horses days prior to the detection of viremia, we have profiled miRNAs at the transcriptome-wide level to comprehensively assess differences between infected and uninfected horses. Next-generation sequencing and the miRDeep2 algorithm identified 742 mature miRNA transcripts corresponding to 593 miRNAs in whole blood of six horses (three HeV-infected, three uninfected). Thirty seven miRNAs were differentially expressed in infected horses, two of which were validated by qRT-PCR. This study describes a methodology for the transcriptome-wide profiling of miRNAs in whole blood and supports the notion that measuring host miRNA expression levels may aid infectious disease diagnosis in the future.Christopher CowledChwan-Hong FooCeline DeffrasnesChristina L. RootesDavid T. WilliamsDeborah MiddletonLin-Fa WangAndrew G. D. BeanCameron R. StewartNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christopher Cowled Chwan-Hong Foo Celine Deffrasnes Christina L. Rootes David T. Williams Deborah Middleton Lin-Fa Wang Andrew G. D. Bean Cameron R. Stewart Circulating microRNA profiles of Hendra virus infection in horses
description	Abstract Hendra virus (HeV) is an emerging zoonotic pathogen harbored by Australian mainland flying foxes. HeV infection can cause lethal disease in humans and horses, and to date all cases of human HeV disease have resulted from contact with infected horses. Currently, diagnosis of acute HeV infections in horses relies on the productive phase of infection when virus shedding may occur. An assay that identifies infected horses during the preclinical phase of infection would reduce the risk of zoonotic viral transmission during management of HeV outbreaks. Having previously shown that the host microRNA (miR)-146a is upregulated in the blood of HeV-infected horses days prior to the detection of viremia, we have profiled miRNAs at the transcriptome-wide level to comprehensively assess differences between infected and uninfected horses. Next-generation sequencing and the miRDeep2 algorithm identified 742 mature miRNA transcripts corresponding to 593 miRNAs in whole blood of six horses (three HeV-infected, three uninfected). Thirty seven miRNAs were differentially expressed in infected horses, two of which were validated by qRT-PCR. This study describes a methodology for the transcriptome-wide profiling of miRNAs in whole blood and supports the notion that measuring host miRNA expression levels may aid infectious disease diagnosis in the future.
format	article
author	Christopher Cowled Chwan-Hong Foo Celine Deffrasnes Christina L. Rootes David T. Williams Deborah Middleton Lin-Fa Wang Andrew G. D. Bean Cameron R. Stewart
author_facet	Christopher Cowled Chwan-Hong Foo Celine Deffrasnes Christina L. Rootes David T. Williams Deborah Middleton Lin-Fa Wang Andrew G. D. Bean Cameron R. Stewart
author_sort	Christopher Cowled
title	Circulating microRNA profiles of Hendra virus infection in horses
title_short	Circulating microRNA profiles of Hendra virus infection in horses
title_full	Circulating microRNA profiles of Hendra virus infection in horses
title_fullStr	Circulating microRNA profiles of Hendra virus infection in horses
title_full_unstemmed	Circulating microRNA profiles of Hendra virus infection in horses
title_sort	circulating microrna profiles of hendra virus infection in horses
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a14697fa40a64d4786ec438ad18eadcf
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Circulating microRNA profiles of Hendra virus infection in horses

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