Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells

In this study, polyethylene glycol (PEG) with hydroxyapatite (HA), with the incorporation of physical gold nanoparticles (AuNPs), was created and equipped through a surface coating technique in order to form PEG-HA-AuNP nanocomposites. The surface morphology and chemical composition were characteriz...

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Autores principales:	Chiung-Chyi Shen, Shan-hui Hsu, Kai-Bo Chang, Chun-An Yeh, Hsiang-Chun Chang, Cheng-Ming Tang, Yi-Chin Yang, Hsien-Hsu Hsieh, Huey-Shan Hung
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	polyethylene glycol hydroxyapatite physical gold nanoparticle mesenchymal stem cells Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a14a46415f944a958b206f1ac8bbc418
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spelling	oai:doaj.org-article:a14a46415f944a958b206f1ac8bbc4182021-11-25T16:49:50ZPhysical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells10.3390/biomedicines91116322227-9059https://doaj.org/article/a14a46415f944a958b206f1ac8bbc4182021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1632https://doaj.org/toc/2227-9059In this study, polyethylene glycol (PEG) with hydroxyapatite (HA), with the incorporation of physical gold nanoparticles (AuNPs), was created and equipped through a surface coating technique in order to form PEG-HA-AuNP nanocomposites. The surface morphology and chemical composition were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), UV–Vis spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and contact angle assessment. The effects of PEG-HA-AuNP nanocomposites on the biocompatibility and biological activity of MC3T3-E1 osteoblast cells, endothelial cells (EC), macrophages (RAW 264.7), and human mesenchymal stem cells (MSCs), as well as the guiding of osteogenic differentiation, were estimated through the use of an in vitro assay. Moreover, the anti-inflammatory, biocompatibility, and endothelialization capacities were further assessed through in vivo evaluation. The PEG-HA-AuNP nanocomposites showed superior biological properties and biocompatibility capacity for cell behavior in both MC3T3-E1 cells and MSCs. These biological events surrounding the cells could be associated with the activation of adhesion, proliferation, migration, and differentiation processes on the PEG-HA-AuNP nanocomposites. Indeed, the induction of the osteogenic differentiation of MSCs by PEG-HA-AuNP nanocomposites and enhanced mineralization activity were also evidenced in this study. Moreover, from the in vivo assay, we further found that PEG-HA-AuNP nanocomposites not only facilitate the anti-immune response, as well as reducing CD86 expression, but also facilitate the endothelialization ability, as well as promoting CD31 expression, when implanted into rats subcutaneously for a period of 1 month. The current research illustrates the potential of PEG-HA-AuNP nanocomposites when used in combination with MSCs for the regeneration of bone tissue, with their nanotopography being employed as an applicable surface modification approach for the fabrication of biomaterials.Chiung-Chyi ShenShan-hui HsuKai-Bo ChangChun-An YehHsiang-Chun ChangCheng-Ming TangYi-Chin YangHsien-Hsu HsiehHuey-Shan HungMDPI AGarticlepolyethylene glycolhydroxyapatitephysical gold nanoparticlemesenchymal stem cellsBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1632, p 1632 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyethylene glycol hydroxyapatite physical gold nanoparticle mesenchymal stem cells Biology (General) QH301-705.5
spellingShingle	polyethylene glycol hydroxyapatite physical gold nanoparticle mesenchymal stem cells Biology (General) QH301-705.5 Chiung-Chyi Shen Shan-hui Hsu Kai-Bo Chang Chun-An Yeh Hsiang-Chun Chang Cheng-Ming Tang Yi-Chin Yang Hsien-Hsu Hsieh Huey-Shan Hung Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells
description	In this study, polyethylene glycol (PEG) with hydroxyapatite (HA), with the incorporation of physical gold nanoparticles (AuNPs), was created and equipped through a surface coating technique in order to form PEG-HA-AuNP nanocomposites. The surface morphology and chemical composition were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), UV–Vis spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and contact angle assessment. The effects of PEG-HA-AuNP nanocomposites on the biocompatibility and biological activity of MC3T3-E1 osteoblast cells, endothelial cells (EC), macrophages (RAW 264.7), and human mesenchymal stem cells (MSCs), as well as the guiding of osteogenic differentiation, were estimated through the use of an in vitro assay. Moreover, the anti-inflammatory, biocompatibility, and endothelialization capacities were further assessed through in vivo evaluation. The PEG-HA-AuNP nanocomposites showed superior biological properties and biocompatibility capacity for cell behavior in both MC3T3-E1 cells and MSCs. These biological events surrounding the cells could be associated with the activation of adhesion, proliferation, migration, and differentiation processes on the PEG-HA-AuNP nanocomposites. Indeed, the induction of the osteogenic differentiation of MSCs by PEG-HA-AuNP nanocomposites and enhanced mineralization activity were also evidenced in this study. Moreover, from the in vivo assay, we further found that PEG-HA-AuNP nanocomposites not only facilitate the anti-immune response, as well as reducing CD86 expression, but also facilitate the endothelialization ability, as well as promoting CD31 expression, when implanted into rats subcutaneously for a period of 1 month. The current research illustrates the potential of PEG-HA-AuNP nanocomposites when used in combination with MSCs for the regeneration of bone tissue, with their nanotopography being employed as an applicable surface modification approach for the fabrication of biomaterials.
format	article
author	Chiung-Chyi Shen Shan-hui Hsu Kai-Bo Chang Chun-An Yeh Hsiang-Chun Chang Cheng-Ming Tang Yi-Chin Yang Hsien-Hsu Hsieh Huey-Shan Hung
author_facet	Chiung-Chyi Shen Shan-hui Hsu Kai-Bo Chang Chun-An Yeh Hsiang-Chun Chang Cheng-Ming Tang Yi-Chin Yang Hsien-Hsu Hsieh Huey-Shan Hung
author_sort	Chiung-Chyi Shen
title	Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells
title_short	Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells
title_full	Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells
title_fullStr	Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells
title_full_unstemmed	Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells
title_sort	physical gold nanoparticle-decorated polyethylene glycol-hydroxyapatite composites guide osteogenesis and angiogenesis of mesenchymal stem cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a14a46415f944a958b206f1ac8bbc418
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Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells

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