Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids

Abstract Rapid infection diagnosis is critical to improving patient treatment and outcome. Recent studies have shown microbial lipids to be sensitive and selective biomarkers for identifying bacterial and fungal species and antimicrobial resistance. Practical procedures for microbial lipid biomarker...

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Autores principales:	Matthew Sorensen, Courtney E. Chandler, Francesca M. Gardner, Salma Ramadan, Prasanna D. Khot, Lisa M. Leung, Christine E. Farrance, David R. Goodlett, Robert K. Ernst, Erik Nilsson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a15136a5d376457a8d05aa37957a3c9b
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spelling	oai:doaj.org-article:a15136a5d376457a8d05aa37957a3c9b2021-12-02T12:33:14ZRapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids10.1038/s41598-020-78401-32045-2322https://doaj.org/article/a15136a5d376457a8d05aa37957a3c9b2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78401-3https://doaj.org/toc/2045-2322Abstract Rapid infection diagnosis is critical to improving patient treatment and outcome. Recent studies have shown microbial lipids to be sensitive and selective biomarkers for identifying bacterial and fungal species and antimicrobial resistance. Practical procedures for microbial lipid biomarker analysis will therefore improve patient outcomes and antimicrobial stewardship. However, current lipid extraction methods require significant hands-on time and are thus not suited for direct adoption as a clinical assay for microbial identification. Here, we have developed a method for lipid extraction directly on the surface of stainless-steel matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) plates, termed fast lipid analysis technique or FLAT, which facilitates the identification of bacterial and fungal species using a sub-60-minute workflow. Additionally, our method detects lipid A modifications in Gram-negative bacteria that are associated with antimicrobial resistance, including to colistin.Matthew SorensenCourtney E. ChandlerFrancesca M. GardnerSalma RamadanPrasanna D. KhotLisa M. LeungChristine E. FarranceDavid R. GoodlettRobert K. ErnstErik NilssonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Matthew Sorensen Courtney E. Chandler Francesca M. Gardner Salma Ramadan Prasanna D. Khot Lisa M. Leung Christine E. Farrance David R. Goodlett Robert K. Ernst Erik Nilsson Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids
description	Abstract Rapid infection diagnosis is critical to improving patient treatment and outcome. Recent studies have shown microbial lipids to be sensitive and selective biomarkers for identifying bacterial and fungal species and antimicrobial resistance. Practical procedures for microbial lipid biomarker analysis will therefore improve patient outcomes and antimicrobial stewardship. However, current lipid extraction methods require significant hands-on time and are thus not suited for direct adoption as a clinical assay for microbial identification. Here, we have developed a method for lipid extraction directly on the surface of stainless-steel matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) plates, termed fast lipid analysis technique or FLAT, which facilitates the identification of bacterial and fungal species using a sub-60-minute workflow. Additionally, our method detects lipid A modifications in Gram-negative bacteria that are associated with antimicrobial resistance, including to colistin.
format	article
author	Matthew Sorensen Courtney E. Chandler Francesca M. Gardner Salma Ramadan Prasanna D. Khot Lisa M. Leung Christine E. Farrance David R. Goodlett Robert K. Ernst Erik Nilsson
author_facet	Matthew Sorensen Courtney E. Chandler Francesca M. Gardner Salma Ramadan Prasanna D. Khot Lisa M. Leung Christine E. Farrance David R. Goodlett Robert K. Ernst Erik Nilsson
author_sort	Matthew Sorensen
title	Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids
title_short	Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids
title_full	Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids
title_fullStr	Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids
title_full_unstemmed	Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids
title_sort	rapid microbial identification and colistin resistance detection via maldi-tof ms using a novel on-target extraction of membrane lipids
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/a15136a5d376457a8d05aa37957a3c9b
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Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids

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