SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus

Biotrophic fungi have to infect their host to obtain nutrients and must establish an interaction with the host to complete their life cycle. In this process, effectors play important roles in manipulating the host’s immune system to avoid being attacked. <i>Sporisorium scitamineum</i> is...

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Autores principales:	Shan Lu, Yukun Wang, Xiaorui Shen, Feng Guo, Chunling Zhou, Ru Li, Baoshan Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Sporisorium scitamineum</i> effector cell function pathogenicity teliospore development Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a154be8a20344648b92017c4e9d4797c
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id	oai:doaj.org-article:a154be8a20344648b92017c4e9d4797c
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spelling	oai:doaj.org-article:a154be8a20344648b92017c4e9d4797c2021-11-25T18:06:07ZSsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus10.3390/jof71109542309-608Xhttps://doaj.org/article/a154be8a20344648b92017c4e9d4797c2021-11-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/954https://doaj.org/toc/2309-608XBiotrophic fungi have to infect their host to obtain nutrients and must establish an interaction with the host to complete their life cycle. In this process, effectors play important roles in manipulating the host’s immune system to avoid being attacked. <i>Sporisorium scitamineum</i> is the causative agent of sugarcane smut, the most important disease in sugarcane-producing regions worldwide. In this work, we functionally characterized the conserved effector PEP1 in <i>S. scitamineum</i>. The mating process and the expression of genes in the MAPK signaling pathway and the <i>a</i> and <i>b</i> loci were adversely affected in <i>Sspep1</i>-null mutants. The requirement for SsPEP1 in pathogenicity and symptom development was allele dosage-dependent, i.e., deleting one <i>Sspep1</i> allele in the mating pair turned a normal black whip with abundant teliospores into a white whip with few teliospores; however, deleting both alleles almost abolished infectivity and whip development. Δ<i>Sspep1</i> mutants produced significantly less mycelium mass within infected plants. Additionally, SsPEP1 was identified as a potent inhibitor of sugarcane POD-1a peroxidase activity, implying that SsPEP1 may function to relieve reactive oxygen species-related stress within the host plant. Taken together, our work demonstrated that SsPEP1 is a multifaceted effector essential for <i>S. scitamineum</i> growth, development, and pathogenicity.Shan LuYukun WangXiaorui ShenFeng GuoChunling ZhouRu LiBaoshan ChenMDPI AGarticle<i>Sporisorium scitamineum</i>effectorcell functionpathogenicityteliospore developmentBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 954, p 954 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Sporisorium scitamineum</i> effector cell function pathogenicity teliospore development Biology (General) QH301-705.5
spellingShingle	<i>Sporisorium scitamineum</i> effector cell function pathogenicity teliospore development Biology (General) QH301-705.5 Shan Lu Yukun Wang Xiaorui Shen Feng Guo Chunling Zhou Ru Li Baoshan Chen SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus
description	Biotrophic fungi have to infect their host to obtain nutrients and must establish an interaction with the host to complete their life cycle. In this process, effectors play important roles in manipulating the host’s immune system to avoid being attacked. <i>Sporisorium scitamineum</i> is the causative agent of sugarcane smut, the most important disease in sugarcane-producing regions worldwide. In this work, we functionally characterized the conserved effector PEP1 in <i>S. scitamineum</i>. The mating process and the expression of genes in the MAPK signaling pathway and the <i>a</i> and <i>b</i> loci were adversely affected in <i>Sspep1</i>-null mutants. The requirement for SsPEP1 in pathogenicity and symptom development was allele dosage-dependent, i.e., deleting one <i>Sspep1</i> allele in the mating pair turned a normal black whip with abundant teliospores into a white whip with few teliospores; however, deleting both alleles almost abolished infectivity and whip development. Δ<i>Sspep1</i> mutants produced significantly less mycelium mass within infected plants. Additionally, SsPEP1 was identified as a potent inhibitor of sugarcane POD-1a peroxidase activity, implying that SsPEP1 may function to relieve reactive oxygen species-related stress within the host plant. Taken together, our work demonstrated that SsPEP1 is a multifaceted effector essential for <i>S. scitamineum</i> growth, development, and pathogenicity.
format	article
author	Shan Lu Yukun Wang Xiaorui Shen Feng Guo Chunling Zhou Ru Li Baoshan Chen
author_facet	Shan Lu Yukun Wang Xiaorui Shen Feng Guo Chunling Zhou Ru Li Baoshan Chen
author_sort	Shan Lu
title	SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus
title_short	SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus
title_full	SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus
title_fullStr	SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus
title_full_unstemmed	SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus
title_sort	sspep1, an effector with essential cellular functions in sugarcane smut fungus
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a154be8a20344648b92017c4e9d4797c
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SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus

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