The overlaying oil type influences in vitro embryo production: differences in composition and compound transfer into incubation medium between oils

Abstract The oil overlay micro-drop system is widely used for cultures of mammalian gametes and embryos. We evaluated hereby the effects of two unaltered commercial oils— Sigma mineral oil (S-MO) and Nidoil paraffin oil (N-PO)—on in vitro embryo production (IVP) outcomes using a pig model. The resul...

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Autores principales:	Cristina A. Martinez, Alicia Nohalez, Inmaculada Parrilla, Miguel Motas, Jordi Roca, Inmaculada Romero, Diego L. García-González, Cristina Cuello, Heriberto Rodriguez-Martinez, Emilio A. Martinez, Maria A. Gil
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a158818e989e4ffd969d32d62d2707a9
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spelling	oai:doaj.org-article:a158818e989e4ffd969d32d62d2707a92021-12-02T15:05:40ZThe overlaying oil type influences in vitro embryo production: differences in composition and compound transfer into incubation medium between oils10.1038/s41598-017-10989-52045-2322https://doaj.org/article/a158818e989e4ffd969d32d62d2707a92017-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-10989-5https://doaj.org/toc/2045-2322Abstract The oil overlay micro-drop system is widely used for cultures of mammalian gametes and embryos. We evaluated hereby the effects of two unaltered commercial oils— Sigma mineral oil (S-MO) and Nidoil paraffin oil (N-PO)—on in vitro embryo production (IVP) outcomes using a pig model. The results showed that while either oil apparently did not affect oocyte maturation and fertilization rates, S-MO negatively affected embryo cleavage rates, blastocyst formation rates, and, consequently, total blastocyst efficiency of the system. No differences in the oxidation state were found between the oils or culture media incubated under S-MO or N-PO. Although both oils slightly differed in elemental composition, there were no differences in the concentrations of elements between fresh media and media incubated under oils. By contrast, we demonstrated clear oil-type differences in both the composition of volatile organic compounds (VOC) and the transfer of some of these VOC´s (straight-chain alkanes and pentanal and 1,3-diethyl benzene) to the culture medium, which could have influenced embryonic development.Cristina A. MartinezAlicia NohalezInmaculada ParrillaMiguel MotasJordi RocaInmaculada RomeroDiego L. García-GonzálezCristina CuelloHeriberto Rodriguez-MartinezEmilio A. MartinezMaria A. GilNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cristina A. Martinez Alicia Nohalez Inmaculada Parrilla Miguel Motas Jordi Roca Inmaculada Romero Diego L. García-González Cristina Cuello Heriberto Rodriguez-Martinez Emilio A. Martinez Maria A. Gil The overlaying oil type influences in vitro embryo production: differences in composition and compound transfer into incubation medium between oils
description	Abstract The oil overlay micro-drop system is widely used for cultures of mammalian gametes and embryos. We evaluated hereby the effects of two unaltered commercial oils— Sigma mineral oil (S-MO) and Nidoil paraffin oil (N-PO)—on in vitro embryo production (IVP) outcomes using a pig model. The results showed that while either oil apparently did not affect oocyte maturation and fertilization rates, S-MO negatively affected embryo cleavage rates, blastocyst formation rates, and, consequently, total blastocyst efficiency of the system. No differences in the oxidation state were found between the oils or culture media incubated under S-MO or N-PO. Although both oils slightly differed in elemental composition, there were no differences in the concentrations of elements between fresh media and media incubated under oils. By contrast, we demonstrated clear oil-type differences in both the composition of volatile organic compounds (VOC) and the transfer of some of these VOC´s (straight-chain alkanes and pentanal and 1,3-diethyl benzene) to the culture medium, which could have influenced embryonic development.
format	article
author	Cristina A. Martinez Alicia Nohalez Inmaculada Parrilla Miguel Motas Jordi Roca Inmaculada Romero Diego L. García-González Cristina Cuello Heriberto Rodriguez-Martinez Emilio A. Martinez Maria A. Gil
author_facet	Cristina A. Martinez Alicia Nohalez Inmaculada Parrilla Miguel Motas Jordi Roca Inmaculada Romero Diego L. García-González Cristina Cuello Heriberto Rodriguez-Martinez Emilio A. Martinez Maria A. Gil
author_sort	Cristina A. Martinez
title	The overlaying oil type influences in vitro embryo production: differences in composition and compound transfer into incubation medium between oils
title_short	The overlaying oil type influences in vitro embryo production: differences in composition and compound transfer into incubation medium between oils
title_full	The overlaying oil type influences in vitro embryo production: differences in composition and compound transfer into incubation medium between oils
title_fullStr	The overlaying oil type influences in vitro embryo production: differences in composition and compound transfer into incubation medium between oils
title_full_unstemmed	The overlaying oil type influences in vitro embryo production: differences in composition and compound transfer into incubation medium between oils
title_sort	overlaying oil type influences in vitro embryo production: differences in composition and compound transfer into incubation medium between oils
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a158818e989e4ffd969d32d62d2707a9
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The overlaying oil type influences in vitro embryo production: differences in composition and compound transfer into incubation medium between oils

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