miRNAs in the Expression Regulation of Dopamine-Related Genes and Proteins in Endometrial Cancer

Disruption of the dopaminergic system leads to many diseases, including cancer. Dopamine and its receptors are involved in the regulation of proliferation, cell death, invasion, and migration. Better understanding of the mechanisms involved in these processes could reveal new molecular markers and t...

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Autores principales:	Michał Czerwiński, Anna Bednarska-Czerwińska, Nikola Zmarzły, Dariusz Boroń, Marcin Oplawski, Beniamin Oskar Grabarek
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	endometrial cancer dopamine dopamine receptors miRNAs Medicine R
Acceso en línea:	https://doaj.org/article/a15be5e953ec4b9f81f5d006ff0b90d5
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id	oai:doaj.org-article:a15be5e953ec4b9f81f5d006ff0b90d5
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spelling	oai:doaj.org-article:a15be5e953ec4b9f81f5d006ff0b90d52021-11-11T17:34:31ZmiRNAs in the Expression Regulation of Dopamine-Related Genes and Proteins in Endometrial Cancer10.3390/jcm102149392077-0383https://doaj.org/article/a15be5e953ec4b9f81f5d006ff0b90d52021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/4939https://doaj.org/toc/2077-0383Disruption of the dopaminergic system leads to many diseases, including cancer. Dopamine and its receptors are involved in the regulation of proliferation, cell death, invasion, and migration. Better understanding of the mechanisms involved in these processes could reveal new molecular markers and therapeutic targets. The aim of this study was to determine the expression profile of dopamine-related genes and proteins in endometrial cancer and to assess whether miRNAs are involved in its regulation. Sixty women were recruited for the study: 30 with endometrial cancer and 30 without cancer. The expression profiles of dopamine-related genes were determined in endometrial tissue samples using microarrays and qRT-PCR. Then, protein concentration was determined with the ELISA test. In the last step, miRNA detection was performed using microarrays. The matching of miRNAs to the studied genes was carried out using the TargetScan tool. The analysis showed DRD2 and DRD3 overexpression, with a reduction in DRD5 expression, which could be due to miR-15a-5p, miR-141-3p, miR-4640-5p, and miR-221-5p activity. High levels of OPRK1 and CXCL12, related to the activity of miR-124-3p.1 and miR-135b-5p, have also been reported. Low COMT expression was probably not associated with miRNA regulation in endometrial cancer.Michał CzerwińskiAnna Bednarska-CzerwińskaNikola ZmarzłyDariusz BorońMarcin OplawskiBeniamin Oskar GrabarekMDPI AGarticleendometrial cancerdopaminedopamine receptorsmiRNAsMedicineRENJournal of Clinical Medicine, Vol 10, Iss 4939, p 4939 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endometrial cancer dopamine dopamine receptors miRNAs Medicine R
spellingShingle	endometrial cancer dopamine dopamine receptors miRNAs Medicine R Michał Czerwiński Anna Bednarska-Czerwińska Nikola Zmarzły Dariusz Boroń Marcin Oplawski Beniamin Oskar Grabarek miRNAs in the Expression Regulation of Dopamine-Related Genes and Proteins in Endometrial Cancer
description	Disruption of the dopaminergic system leads to many diseases, including cancer. Dopamine and its receptors are involved in the regulation of proliferation, cell death, invasion, and migration. Better understanding of the mechanisms involved in these processes could reveal new molecular markers and therapeutic targets. The aim of this study was to determine the expression profile of dopamine-related genes and proteins in endometrial cancer and to assess whether miRNAs are involved in its regulation. Sixty women were recruited for the study: 30 with endometrial cancer and 30 without cancer. The expression profiles of dopamine-related genes were determined in endometrial tissue samples using microarrays and qRT-PCR. Then, protein concentration was determined with the ELISA test. In the last step, miRNA detection was performed using microarrays. The matching of miRNAs to the studied genes was carried out using the TargetScan tool. The analysis showed DRD2 and DRD3 overexpression, with a reduction in DRD5 expression, which could be due to miR-15a-5p, miR-141-3p, miR-4640-5p, and miR-221-5p activity. High levels of OPRK1 and CXCL12, related to the activity of miR-124-3p.1 and miR-135b-5p, have also been reported. Low COMT expression was probably not associated with miRNA regulation in endometrial cancer.
format	article
author	Michał Czerwiński Anna Bednarska-Czerwińska Nikola Zmarzły Dariusz Boroń Marcin Oplawski Beniamin Oskar Grabarek
author_facet	Michał Czerwiński Anna Bednarska-Czerwińska Nikola Zmarzły Dariusz Boroń Marcin Oplawski Beniamin Oskar Grabarek
author_sort	Michał Czerwiński
title	miRNAs in the Expression Regulation of Dopamine-Related Genes and Proteins in Endometrial Cancer
title_short	miRNAs in the Expression Regulation of Dopamine-Related Genes and Proteins in Endometrial Cancer
title_full	miRNAs in the Expression Regulation of Dopamine-Related Genes and Proteins in Endometrial Cancer
title_fullStr	miRNAs in the Expression Regulation of Dopamine-Related Genes and Proteins in Endometrial Cancer
title_full_unstemmed	miRNAs in the Expression Regulation of Dopamine-Related Genes and Proteins in Endometrial Cancer
title_sort	mirnas in the expression regulation of dopamine-related genes and proteins in endometrial cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a15be5e953ec4b9f81f5d006ff0b90d5
work_keys_str_mv	AT michałczerwinski mirnasintheexpressionregulationofdopaminerelatedgenesandproteinsinendometrialcancer AT annabednarskaczerwinska mirnasintheexpressionregulationofdopaminerelatedgenesandproteinsinendometrialcancer AT nikolazmarzły mirnasintheexpressionregulationofdopaminerelatedgenesandproteinsinendometrialcancer AT dariuszboron mirnasintheexpressionregulationofdopaminerelatedgenesandproteinsinendometrialcancer AT marcinoplawski mirnasintheexpressionregulationofdopaminerelatedgenesandproteinsinendometrialcancer AT beniaminoskargrabarek mirnasintheexpressionregulationofdopaminerelatedgenesandproteinsinendometrialcancer
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miRNAs in the Expression Regulation of Dopamine-Related Genes and Proteins in Endometrial Cancer

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