Para-aminobenzoic acid (PABA) synthase enhances thermotolerance of mushroom Agaricus bisporus.

Most mushrooms are thermo-sensitive to temperatures over 23°C, which greatly restricts their agricultural cultivation. Understanding mushroom's innate heat-tolerance mechanisms may facilitate genetic improvements of their thermotolerance. Agaricus bisporus strain 02 is a relatively thermotolera...

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Autores principales:	Zhonglei Lu, Xiangxiang Kong, Zhaoming Lu, Meixiang Xiao, Meiyuan Chen, Liang Zhu, Yuemao Shen, Xiangyang Hu, Siyang Song
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a15e07b398ad4925a9fadad224678549
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spelling	oai:doaj.org-article:a15e07b398ad4925a9fadad2246785492021-11-18T08:29:02ZPara-aminobenzoic acid (PABA) synthase enhances thermotolerance of mushroom Agaricus bisporus.1932-620310.1371/journal.pone.0091298https://doaj.org/article/a15e07b398ad4925a9fadad2246785492014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24614118/?tool=EBIhttps://doaj.org/toc/1932-6203Most mushrooms are thermo-sensitive to temperatures over 23°C, which greatly restricts their agricultural cultivation. Understanding mushroom's innate heat-tolerance mechanisms may facilitate genetic improvements of their thermotolerance. Agaricus bisporus strain 02 is a relatively thermotolerant mushroom strain, while strain 8213 is quite thermo-sensitive. Here, we compared their responses at proteomic level to heat treatment at 33°C. We identified 73 proteins that are differentially expressed between 02 and 8213 or induced upon heat stress in strain 02 itself, 48 of which with a known identity. Among them, 4 proteins are constitutively more highly expressed in 02 than 8213; and they can be further upregulated in response to heat stress in 02, but not in 8213. One protein is encoded by the para-aminobenzoic acid (PABA) synthase gene Pabs, which has been shown to scavenge the reactive oxygen species in vitro. Pabs mRNA and its chemical product PABA show similar heat stress induction pattern as PABA synthase protein and are more abundant in 02, indicating transcriptional level upregulation of Pabs upon heat stress. A specific inhibitor of PABA synthesis impaired thermotolerance of 02, while exogenous PABA or transgenic overexpression of 02 derived PABA synthase enhanced thermotolerance of 8213. Furthermore, compared to 8213, 02 accumulated less H2O2 but more defense-related proteins (e.g., HSPs and Chitinase) under heat stress. Together, these results demonstrate a role of PABA in enhancing mushroom thermotolerance by removing H2O2 and elevating defense-related proteins.Zhonglei LuXiangxiang KongZhaoming LuMeixiang XiaoMeiyuan ChenLiang ZhuYuemao ShenXiangyang HuSiyang SongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91298 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhonglei Lu Xiangxiang Kong Zhaoming Lu Meixiang Xiao Meiyuan Chen Liang Zhu Yuemao Shen Xiangyang Hu Siyang Song Para-aminobenzoic acid (PABA) synthase enhances thermotolerance of mushroom Agaricus bisporus.
description	Most mushrooms are thermo-sensitive to temperatures over 23°C, which greatly restricts their agricultural cultivation. Understanding mushroom's innate heat-tolerance mechanisms may facilitate genetic improvements of their thermotolerance. Agaricus bisporus strain 02 is a relatively thermotolerant mushroom strain, while strain 8213 is quite thermo-sensitive. Here, we compared their responses at proteomic level to heat treatment at 33°C. We identified 73 proteins that are differentially expressed between 02 and 8213 or induced upon heat stress in strain 02 itself, 48 of which with a known identity. Among them, 4 proteins are constitutively more highly expressed in 02 than 8213; and they can be further upregulated in response to heat stress in 02, but not in 8213. One protein is encoded by the para-aminobenzoic acid (PABA) synthase gene Pabs, which has been shown to scavenge the reactive oxygen species in vitro. Pabs mRNA and its chemical product PABA show similar heat stress induction pattern as PABA synthase protein and are more abundant in 02, indicating transcriptional level upregulation of Pabs upon heat stress. A specific inhibitor of PABA synthesis impaired thermotolerance of 02, while exogenous PABA or transgenic overexpression of 02 derived PABA synthase enhanced thermotolerance of 8213. Furthermore, compared to 8213, 02 accumulated less H2O2 but more defense-related proteins (e.g., HSPs and Chitinase) under heat stress. Together, these results demonstrate a role of PABA in enhancing mushroom thermotolerance by removing H2O2 and elevating defense-related proteins.
format	article
author	Zhonglei Lu Xiangxiang Kong Zhaoming Lu Meixiang Xiao Meiyuan Chen Liang Zhu Yuemao Shen Xiangyang Hu Siyang Song
author_facet	Zhonglei Lu Xiangxiang Kong Zhaoming Lu Meixiang Xiao Meiyuan Chen Liang Zhu Yuemao Shen Xiangyang Hu Siyang Song
author_sort	Zhonglei Lu
title	Para-aminobenzoic acid (PABA) synthase enhances thermotolerance of mushroom Agaricus bisporus.
title_short	Para-aminobenzoic acid (PABA) synthase enhances thermotolerance of mushroom Agaricus bisporus.
title_full	Para-aminobenzoic acid (PABA) synthase enhances thermotolerance of mushroom Agaricus bisporus.
title_fullStr	Para-aminobenzoic acid (PABA) synthase enhances thermotolerance of mushroom Agaricus bisporus.
title_full_unstemmed	Para-aminobenzoic acid (PABA) synthase enhances thermotolerance of mushroom Agaricus bisporus.
title_sort	para-aminobenzoic acid (paba) synthase enhances thermotolerance of mushroom agaricus bisporus.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/a15e07b398ad4925a9fadad224678549
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Para-aminobenzoic acid (PABA) synthase enhances thermotolerance of mushroom Agaricus bisporus.

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