Evaluation of the long-term effect of polyhexamethylene guanidine phosphate in a rat lung model using conventional chest computed tomography with histopathologic analysis.

There have been no studies on the effects of polyhexamethylene guanidine phosphate (PHMG) after a long period of exposure in the rodent model. We aimed to evaluate long-term lung damage after PHMG exposure using conventional chest computed tomography (CT) and histopathologic analysis in a rat model....

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Autores principales:	Cherry Kim, Sang Hoon Jeong, Jaeyoung Kim, Ja Young Kang, Yoon Jeong Nam, Ariunaa Togloom, Jaehyung Cha, Ki Yeol Lee, Chang Hyun Lee, Eun-Kee Park, Ju-Han Lee
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a1673d60a5674b0ea3c849115d62b83f
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spelling	oai:doaj.org-article:a1673d60a5674b0ea3c849115d62b83f2021-12-02T20:08:28ZEvaluation of the long-term effect of polyhexamethylene guanidine phosphate in a rat lung model using conventional chest computed tomography with histopathologic analysis.1932-620310.1371/journal.pone.0256756https://doaj.org/article/a1673d60a5674b0ea3c849115d62b83f2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256756https://doaj.org/toc/1932-6203There have been no studies on the effects of polyhexamethylene guanidine phosphate (PHMG) after a long period of exposure in the rodent model. We aimed to evaluate long-term lung damage after PHMG exposure using conventional chest computed tomography (CT) and histopathologic analysis in a rat model. A PHMG solution was intratracheally administrated to 24 male rats. At 8, 26, and 52 weeks after PHMG instillation, conventional chest CT was performed in all rats and both lungs were extracted for histopathologic evaluation. At 52 weeks after PHMG instillation, four carcinomas had developed in three of the eight rats (37.5%). Bronchiolo-alveolar hyperplasia and adenoma were found in rats at 8, 26, and 52 weeks post-instillation. The number of bronchiolo-alveolar hyperplasia significantly increased over time (P-value for trend< 0.001). The severity of lung fibrosis and fibrosis scores significantly increased over time (P-values for trend = 0.002 and 0.023, respectively). Conventional chest CT analysis showed that bronchiectasis and linear density scores suggestive of fibrosis significantly increased over time (P-value for trend < 0.001). Our study revealed that one instillation of PHMG in a rat model resulted in lung carcinomas and progressive and irreversible fibrosis one year later based on conventional chest CT and histopathologic analysis. PHMG may be a lung carcinogen in the rat model.Cherry KimSang Hoon JeongJaeyoung KimJa Young KangYoon Jeong NamAriunaa TogloomJaehyung ChaKi Yeol LeeChang Hyun LeeEun-Kee ParkJu-Han LeePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256756 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cherry Kim Sang Hoon Jeong Jaeyoung Kim Ja Young Kang Yoon Jeong Nam Ariunaa Togloom Jaehyung Cha Ki Yeol Lee Chang Hyun Lee Eun-Kee Park Ju-Han Lee Evaluation of the long-term effect of polyhexamethylene guanidine phosphate in a rat lung model using conventional chest computed tomography with histopathologic analysis.
description	There have been no studies on the effects of polyhexamethylene guanidine phosphate (PHMG) after a long period of exposure in the rodent model. We aimed to evaluate long-term lung damage after PHMG exposure using conventional chest computed tomography (CT) and histopathologic analysis in a rat model. A PHMG solution was intratracheally administrated to 24 male rats. At 8, 26, and 52 weeks after PHMG instillation, conventional chest CT was performed in all rats and both lungs were extracted for histopathologic evaluation. At 52 weeks after PHMG instillation, four carcinomas had developed in three of the eight rats (37.5%). Bronchiolo-alveolar hyperplasia and adenoma were found in rats at 8, 26, and 52 weeks post-instillation. The number of bronchiolo-alveolar hyperplasia significantly increased over time (P-value for trend< 0.001). The severity of lung fibrosis and fibrosis scores significantly increased over time (P-values for trend = 0.002 and 0.023, respectively). Conventional chest CT analysis showed that bronchiectasis and linear density scores suggestive of fibrosis significantly increased over time (P-value for trend < 0.001). Our study revealed that one instillation of PHMG in a rat model resulted in lung carcinomas and progressive and irreversible fibrosis one year later based on conventional chest CT and histopathologic analysis. PHMG may be a lung carcinogen in the rat model.
format	article
author	Cherry Kim Sang Hoon Jeong Jaeyoung Kim Ja Young Kang Yoon Jeong Nam Ariunaa Togloom Jaehyung Cha Ki Yeol Lee Chang Hyun Lee Eun-Kee Park Ju-Han Lee
author_facet	Cherry Kim Sang Hoon Jeong Jaeyoung Kim Ja Young Kang Yoon Jeong Nam Ariunaa Togloom Jaehyung Cha Ki Yeol Lee Chang Hyun Lee Eun-Kee Park Ju-Han Lee
author_sort	Cherry Kim
title	Evaluation of the long-term effect of polyhexamethylene guanidine phosphate in a rat lung model using conventional chest computed tomography with histopathologic analysis.
title_short	Evaluation of the long-term effect of polyhexamethylene guanidine phosphate in a rat lung model using conventional chest computed tomography with histopathologic analysis.
title_full	Evaluation of the long-term effect of polyhexamethylene guanidine phosphate in a rat lung model using conventional chest computed tomography with histopathologic analysis.
title_fullStr	Evaluation of the long-term effect of polyhexamethylene guanidine phosphate in a rat lung model using conventional chest computed tomography with histopathologic analysis.
title_full_unstemmed	Evaluation of the long-term effect of polyhexamethylene guanidine phosphate in a rat lung model using conventional chest computed tomography with histopathologic analysis.
title_sort	evaluation of the long-term effect of polyhexamethylene guanidine phosphate in a rat lung model using conventional chest computed tomography with histopathologic analysis.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a1673d60a5674b0ea3c849115d62b83f
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Evaluation of the long-term effect of polyhexamethylene guanidine phosphate in a rat lung model using conventional chest computed tomography with histopathologic analysis.

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