Functional domains of androgen receptor coactivator p44/Mep50/WDR77and its interaction with Smad1.
p44/MEP50/WDR77 has been identified as a coactivator of androgen receptor (AR), with distinct growth suppression and promotion function in gender specific endocrine organs and their malignancies. We dissected the functional domains of p44 for protein interaction with transcription factors, transcrip...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/a171e214df7948f19f0b7260eaa0733f |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:a171e214df7948f19f0b7260eaa0733f |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:a171e214df7948f19f0b7260eaa0733f2021-11-18T07:43:55ZFunctional domains of androgen receptor coactivator p44/Mep50/WDR77and its interaction with Smad1.1932-620310.1371/journal.pone.0064663https://doaj.org/article/a171e214df7948f19f0b7260eaa0733f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23734213/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203p44/MEP50/WDR77 has been identified as a coactivator of androgen receptor (AR), with distinct growth suppression and promotion function in gender specific endocrine organs and their malignancies. We dissected the functional domains of p44 for protein interaction with transcription factors, transcriptional activation, as well as the functional domains in p44 related to its growth inhibition in prostate cancer. Using a yeast two-hybrid screen, we identified a novel transcription complex AR-p44-Smad1, confirmed for physical interaction by co-immunoprecipitaion and functional interaction with luciferase assays in human prostate cancer cells. Yeast two-hybrid assay revealed that the N-terminal region of p44, instead of the traditional WD40 domain at the C-terminus, mediates the interaction among p44, N-terminus of AR and full length Smad1. Although both N and C terminal domains of p44 are necessary for maximum AR transcriptional activation, the N terminal fragment of p44 alone maintains the basic effect on AR transcriptional activation. Cell proliferation assays with N- and C- terminal deletion mutations indicated that the central portion of p44 is required for nuclear p44 mediated prostate cancer growth inhibition.Yirong LiLiantian TianMartin LigrGarrett DanielsYi PengXinyu WuMandeep SinghJianjun WeiYongzhao ShaoHerbert LeporRuliang XuZhijie ChangZhengxin WangPeng LeePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e64663 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Yirong Li Liantian Tian Martin Ligr Garrett Daniels Yi Peng Xinyu Wu Mandeep Singh Jianjun Wei Yongzhao Shao Herbert Lepor Ruliang Xu Zhijie Chang Zhengxin Wang Peng Lee Functional domains of androgen receptor coactivator p44/Mep50/WDR77and its interaction with Smad1. |
| description |
p44/MEP50/WDR77 has been identified as a coactivator of androgen receptor (AR), with distinct growth suppression and promotion function in gender specific endocrine organs and their malignancies. We dissected the functional domains of p44 for protein interaction with transcription factors, transcriptional activation, as well as the functional domains in p44 related to its growth inhibition in prostate cancer. Using a yeast two-hybrid screen, we identified a novel transcription complex AR-p44-Smad1, confirmed for physical interaction by co-immunoprecipitaion and functional interaction with luciferase assays in human prostate cancer cells. Yeast two-hybrid assay revealed that the N-terminal region of p44, instead of the traditional WD40 domain at the C-terminus, mediates the interaction among p44, N-terminus of AR and full length Smad1. Although both N and C terminal domains of p44 are necessary for maximum AR transcriptional activation, the N terminal fragment of p44 alone maintains the basic effect on AR transcriptional activation. Cell proliferation assays with N- and C- terminal deletion mutations indicated that the central portion of p44 is required for nuclear p44 mediated prostate cancer growth inhibition. |
| format |
article |
| author |
Yirong Li Liantian Tian Martin Ligr Garrett Daniels Yi Peng Xinyu Wu Mandeep Singh Jianjun Wei Yongzhao Shao Herbert Lepor Ruliang Xu Zhijie Chang Zhengxin Wang Peng Lee |
| author_facet |
Yirong Li Liantian Tian Martin Ligr Garrett Daniels Yi Peng Xinyu Wu Mandeep Singh Jianjun Wei Yongzhao Shao Herbert Lepor Ruliang Xu Zhijie Chang Zhengxin Wang Peng Lee |
| author_sort |
Yirong Li |
| title |
Functional domains of androgen receptor coactivator p44/Mep50/WDR77and its interaction with Smad1. |
| title_short |
Functional domains of androgen receptor coactivator p44/Mep50/WDR77and its interaction with Smad1. |
| title_full |
Functional domains of androgen receptor coactivator p44/Mep50/WDR77and its interaction with Smad1. |
| title_fullStr |
Functional domains of androgen receptor coactivator p44/Mep50/WDR77and its interaction with Smad1. |
| title_full_unstemmed |
Functional domains of androgen receptor coactivator p44/Mep50/WDR77and its interaction with Smad1. |
| title_sort |
functional domains of androgen receptor coactivator p44/mep50/wdr77and its interaction with smad1. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/a171e214df7948f19f0b7260eaa0733f |
| work_keys_str_mv |
AT yirongli functionaldomainsofandrogenreceptorcoactivatorp44mep50wdr77anditsinteractionwithsmad1 AT liantiantian functionaldomainsofandrogenreceptorcoactivatorp44mep50wdr77anditsinteractionwithsmad1 AT martinligr functionaldomainsofandrogenreceptorcoactivatorp44mep50wdr77anditsinteractionwithsmad1 AT garrettdaniels functionaldomainsofandrogenreceptorcoactivatorp44mep50wdr77anditsinteractionwithsmad1 AT yipeng functionaldomainsofandrogenreceptorcoactivatorp44mep50wdr77anditsinteractionwithsmad1 AT xinyuwu functionaldomainsofandrogenreceptorcoactivatorp44mep50wdr77anditsinteractionwithsmad1 AT mandeepsingh functionaldomainsofandrogenreceptorcoactivatorp44mep50wdr77anditsinteractionwithsmad1 AT jianjunwei functionaldomainsofandrogenreceptorcoactivatorp44mep50wdr77anditsinteractionwithsmad1 AT yongzhaoshao functionaldomainsofandrogenreceptorcoactivatorp44mep50wdr77anditsinteractionwithsmad1 AT herbertlepor functionaldomainsofandrogenreceptorcoactivatorp44mep50wdr77anditsinteractionwithsmad1 AT ruliangxu functionaldomainsofandrogenreceptorcoactivatorp44mep50wdr77anditsinteractionwithsmad1 AT zhijiechang functionaldomainsofandrogenreceptorcoactivatorp44mep50wdr77anditsinteractionwithsmad1 AT zhengxinwang functionaldomainsofandrogenreceptorcoactivatorp44mep50wdr77anditsinteractionwithsmad1 AT penglee functionaldomainsofandrogenreceptorcoactivatorp44mep50wdr77anditsinteractionwithsmad1 |
| _version_ |
1718423053724024832 |