Zebrafish automatic monitoring system for conditioning and behavioral analysis

Abstract Studies using zebrafish (Danio rerio) in neuro-behavioural research are growing. Measuring fish behavior by computational methods is one of the most efficient ways to avoid human bias in experimental analyses, extending them to various approaches. Sometimes, thorough analyses are difficult...

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Autores principales:	Marta de Oliveira Barreiros, Felipe Gomes Barbosa, Diego de Oliveira Dantas, Daniel de Matos Luna dos Santos, Sidarta Ribeiro, Giselle Cutrim de Oliveira Santos, Allan Kardec Barros
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a17609aef03846499c168c637244977c
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spelling	oai:doaj.org-article:a17609aef03846499c168c637244977c2021-12-02T13:41:22ZZebrafish automatic monitoring system for conditioning and behavioral analysis10.1038/s41598-021-87502-62045-2322https://doaj.org/article/a17609aef03846499c168c637244977c2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87502-6https://doaj.org/toc/2045-2322Abstract Studies using zebrafish (Danio rerio) in neuro-behavioural research are growing. Measuring fish behavior by computational methods is one of the most efficient ways to avoid human bias in experimental analyses, extending them to various approaches. Sometimes, thorough analyses are difficult to do, as fish can behave unpredictably during an experimental strategy. However, the analyses can be implemented in an automated way, using an online strategy and video processing for a complete assessment of the zebrafish behavior, based on the detection and tracking of fish during an activity. Here, a fully automatic conditioning and detailed analysis of zebrafish behavior is presented. Microcontrolled components were used to control the delivery of visual and sound stimuli, in addition to the concise amounts of food after conditioned stimuli for adult zebrafish groups in a conventional tank. The images were captured and processed for automatic detection of the fish, and the training of the fish was done in two evaluation strategies: simple and complex. In simple conditioning, the zebrafish showed significant responses from the second attempt, learning that the conditioned stimulus was a predictor of food presentation in a specific space of the tank, where the food was dumped. When the fish were subjected to two stimuli for decision-making in the food reward, the zebrafish obtained better responses to red light stimuli in relation to vibration. The behavior change was clear in stimulated fish in relation to the control group, thus, the distances traveled and the speed were greater, while the polarization was lower in stimulated fish. This automated system allows for the conditioning and assessment of zebrafish behavior online, with greater stability in experiments, and in the analysis of the behavior of individual fish or fish schools, including learning and memory studies.Marta de Oliveira BarreirosFelipe Gomes BarbosaDiego de Oliveira DantasDaniel de Matos Luna dos SantosSidarta RibeiroGiselle Cutrim de Oliveira SantosAllan Kardec BarrosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marta de Oliveira Barreiros Felipe Gomes Barbosa Diego de Oliveira Dantas Daniel de Matos Luna dos Santos Sidarta Ribeiro Giselle Cutrim de Oliveira Santos Allan Kardec Barros Zebrafish automatic monitoring system for conditioning and behavioral analysis
description	Abstract Studies using zebrafish (Danio rerio) in neuro-behavioural research are growing. Measuring fish behavior by computational methods is one of the most efficient ways to avoid human bias in experimental analyses, extending them to various approaches. Sometimes, thorough analyses are difficult to do, as fish can behave unpredictably during an experimental strategy. However, the analyses can be implemented in an automated way, using an online strategy and video processing for a complete assessment of the zebrafish behavior, based on the detection and tracking of fish during an activity. Here, a fully automatic conditioning and detailed analysis of zebrafish behavior is presented. Microcontrolled components were used to control the delivery of visual and sound stimuli, in addition to the concise amounts of food after conditioned stimuli for adult zebrafish groups in a conventional tank. The images were captured and processed for automatic detection of the fish, and the training of the fish was done in two evaluation strategies: simple and complex. In simple conditioning, the zebrafish showed significant responses from the second attempt, learning that the conditioned stimulus was a predictor of food presentation in a specific space of the tank, where the food was dumped. When the fish were subjected to two stimuli for decision-making in the food reward, the zebrafish obtained better responses to red light stimuli in relation to vibration. The behavior change was clear in stimulated fish in relation to the control group, thus, the distances traveled and the speed were greater, while the polarization was lower in stimulated fish. This automated system allows for the conditioning and assessment of zebrafish behavior online, with greater stability in experiments, and in the analysis of the behavior of individual fish or fish schools, including learning and memory studies.
format	article
author	Marta de Oliveira Barreiros Felipe Gomes Barbosa Diego de Oliveira Dantas Daniel de Matos Luna dos Santos Sidarta Ribeiro Giselle Cutrim de Oliveira Santos Allan Kardec Barros
author_facet	Marta de Oliveira Barreiros Felipe Gomes Barbosa Diego de Oliveira Dantas Daniel de Matos Luna dos Santos Sidarta Ribeiro Giselle Cutrim de Oliveira Santos Allan Kardec Barros
author_sort	Marta de Oliveira Barreiros
title	Zebrafish automatic monitoring system for conditioning and behavioral analysis
title_short	Zebrafish automatic monitoring system for conditioning and behavioral analysis
title_full	Zebrafish automatic monitoring system for conditioning and behavioral analysis
title_fullStr	Zebrafish automatic monitoring system for conditioning and behavioral analysis
title_full_unstemmed	Zebrafish automatic monitoring system for conditioning and behavioral analysis
title_sort	zebrafish automatic monitoring system for conditioning and behavioral analysis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a17609aef03846499c168c637244977c
work_keys_str_mv	AT martadeoliveirabarreiros zebrafishautomaticmonitoringsystemforconditioningandbehavioralanalysis AT felipegomesbarbosa zebrafishautomaticmonitoringsystemforconditioningandbehavioralanalysis AT diegodeoliveiradantas zebrafishautomaticmonitoringsystemforconditioningandbehavioralanalysis AT danieldematoslunadossantos zebrafishautomaticmonitoringsystemforconditioningandbehavioralanalysis AT sidartaribeiro zebrafishautomaticmonitoringsystemforconditioningandbehavioralanalysis AT gisellecutrimdeoliveirasantos zebrafishautomaticmonitoringsystemforconditioningandbehavioralanalysis AT allankardecbarros zebrafishautomaticmonitoringsystemforconditioningandbehavioralanalysis
_version_	1718392571929034752

Zebrafish automatic monitoring system for conditioning and behavioral analysis

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