Effects of soil texture and nitrogen fertilisation on soil bacterial community structure and nitrogen uptake in flue-cured tobacco

Abstract We tested the hypothesis that soil texture and nitrogen (N) fertilisation are the primary factors regulating the N cycle and soil bacterial community structure. The response of soil bacterial communities to N fertilisation in different textured soils might help in identifying the specific u...

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Autores principales:	Meiying Zheng, Pei Zhu, Jiayu Zheng, Lin Xue, Qifa Zhu, Xianjie Cai, Sen Cheng, Zhongfeng Zhang, Fanyu Kong, Jiguang Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a17c440bf198447c96be85d8f727a316
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spelling	oai:doaj.org-article:a17c440bf198447c96be85d8f727a3162021-11-28T12:16:46ZEffects of soil texture and nitrogen fertilisation on soil bacterial community structure and nitrogen uptake in flue-cured tobacco10.1038/s41598-021-01957-12045-2322https://doaj.org/article/a17c440bf198447c96be85d8f727a3162021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01957-1https://doaj.org/toc/2045-2322Abstract We tested the hypothesis that soil texture and nitrogen (N) fertilisation are the primary factors regulating the N cycle and soil bacterial community structure. The response of soil bacterial communities to N fertilisation in different textured soils might help in identifying the specific underlying mechanism and hence management of N fertiliser application in fields. We examined how N fertiliser accumulates in flue-cured tobacco and influences soil bacterial community structure in different textured soils. We conducted plot and micro-plot experimental measurements of N content in soil and tobacco samples using the KNO3 15N isotope technique. Soil bacterial community structure was determined using high-throughput sequencing of 16S rRNA. Nitrogen absorption and utilisation by tobacco plants were highest in sandy loam soils, followed by loam soil and clay loam. The ability of clay loam to supply N was weak during the plant growth period. Absence of fertilisation could reduce bacterial abundance in soils to various degrees. Bacterial diversity was higher in sandy loam soil than in loam soil and clay loam. Soil texture and N fertilisation significantly affected soil bacterial community structure and diversity. Proteobacteria, Acidobacteria, Firmicutes, Bacteroidetes, Actinobacteria, and Chloroflexi were the dominant bacterial phyla, while Bacillus, Nitrobacter, Nitrosospira, Nitrospira, and Rhizobium were the primary N transformation bacteria at the genus level in all treatments. However, relative abundances differed with N fertiliser application, which could lead to differential N availability and N use efficiency of tobacco among soil types. We conclude that both soil texture and N fertilisation influence N accumulation and distribution in flue-cured tobacco and thus regulate soil bacterial communities. N fertiliser application in sandy loam soil should be strictly controlled for its higher N use efficiency, soil bacterial abundance, and diversity.Meiying ZhengPei ZhuJiayu ZhengLin XueQifa ZhuXianjie CaiSen ChengZhongfeng ZhangFanyu KongJiguang ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meiying Zheng Pei Zhu Jiayu Zheng Lin Xue Qifa Zhu Xianjie Cai Sen Cheng Zhongfeng Zhang Fanyu Kong Jiguang Zhang Effects of soil texture and nitrogen fertilisation on soil bacterial community structure and nitrogen uptake in flue-cured tobacco
description	Abstract We tested the hypothesis that soil texture and nitrogen (N) fertilisation are the primary factors regulating the N cycle and soil bacterial community structure. The response of soil bacterial communities to N fertilisation in different textured soils might help in identifying the specific underlying mechanism and hence management of N fertiliser application in fields. We examined how N fertiliser accumulates in flue-cured tobacco and influences soil bacterial community structure in different textured soils. We conducted plot and micro-plot experimental measurements of N content in soil and tobacco samples using the KNO3 15N isotope technique. Soil bacterial community structure was determined using high-throughput sequencing of 16S rRNA. Nitrogen absorption and utilisation by tobacco plants were highest in sandy loam soils, followed by loam soil and clay loam. The ability of clay loam to supply N was weak during the plant growth period. Absence of fertilisation could reduce bacterial abundance in soils to various degrees. Bacterial diversity was higher in sandy loam soil than in loam soil and clay loam. Soil texture and N fertilisation significantly affected soil bacterial community structure and diversity. Proteobacteria, Acidobacteria, Firmicutes, Bacteroidetes, Actinobacteria, and Chloroflexi were the dominant bacterial phyla, while Bacillus, Nitrobacter, Nitrosospira, Nitrospira, and Rhizobium were the primary N transformation bacteria at the genus level in all treatments. However, relative abundances differed with N fertiliser application, which could lead to differential N availability and N use efficiency of tobacco among soil types. We conclude that both soil texture and N fertilisation influence N accumulation and distribution in flue-cured tobacco and thus regulate soil bacterial communities. N fertiliser application in sandy loam soil should be strictly controlled for its higher N use efficiency, soil bacterial abundance, and diversity.
format	article
author	Meiying Zheng Pei Zhu Jiayu Zheng Lin Xue Qifa Zhu Xianjie Cai Sen Cheng Zhongfeng Zhang Fanyu Kong Jiguang Zhang
author_facet	Meiying Zheng Pei Zhu Jiayu Zheng Lin Xue Qifa Zhu Xianjie Cai Sen Cheng Zhongfeng Zhang Fanyu Kong Jiguang Zhang
author_sort	Meiying Zheng
title	Effects of soil texture and nitrogen fertilisation on soil bacterial community structure and nitrogen uptake in flue-cured tobacco
title_short	Effects of soil texture and nitrogen fertilisation on soil bacterial community structure and nitrogen uptake in flue-cured tobacco
title_full	Effects of soil texture and nitrogen fertilisation on soil bacterial community structure and nitrogen uptake in flue-cured tobacco
title_fullStr	Effects of soil texture and nitrogen fertilisation on soil bacterial community structure and nitrogen uptake in flue-cured tobacco
title_full_unstemmed	Effects of soil texture and nitrogen fertilisation on soil bacterial community structure and nitrogen uptake in flue-cured tobacco
title_sort	effects of soil texture and nitrogen fertilisation on soil bacterial community structure and nitrogen uptake in flue-cured tobacco
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a17c440bf198447c96be85d8f727a316
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Effects of soil texture and nitrogen fertilisation on soil bacterial community structure and nitrogen uptake in flue-cured tobacco

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