Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines

Abstract. Fadhilah K, Wahyuono S, Astuti P. 2021. Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines. Biodiversitas 22: 3743-3748. Lansium domesticum (Fam. Meliaceae), a tropical fruit (local name, Duku), has been repor...

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Autores principales:	Khusnul Fadhilah, Subagus Wahyuono, Puji Astuti
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	cytotoxic t47d hepg2 lansium domesticum Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a183014bc2ce4e4e8e88d142fbdb443d
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spelling	oai:doaj.org-article:a183014bc2ce4e4e8e88d142fbdb443d2021-11-22T12:18:55ZFractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines1412-033X2085-472210.13057/biodiv/d220918https://doaj.org/article/a183014bc2ce4e4e8e88d142fbdb443d2021-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/8941https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Fadhilah K, Wahyuono S, Astuti P. 2021. Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines. Biodiversitas 22: 3743-3748. Lansium domesticum (Fam. Meliaceae), a tropical fruit (local name, Duku), has been reported to have various biological activities. At the moment we emphasize on searching compounds to have cytotoxic activity from the peel of L. domesticum. The study was initiated by extraction with EtOAc followed by fractionation that was monitored by thin-layer chromatography (TLC) and cytotoxicity against T-47D and HepG2 cells. The EtOAc extract of the sample was triturated with n-hexane to give n-hexane soluble (A) n-hexane insoluble fractions (B). The B fraction was fractionated by vacuum column chromatography using gradient solvent composition of n-hexane: acetone to give 18 fractions. According to TLC similarity pictures, fractions were combined to give 6 fractions (I-VI). Isolation was performed using preparative TLC, and the cytotoxic assay was performed using MTT method. The isolated compound was identified as a sesquiterpene having one aldehyde functional group, based on spectroscopic data.The isolated compound displayed cytotoxic activity on T-47D (IC50, 48.58 + 0.96 µg/mL) and HepG2 (IC50, 127.45 + 25.76 µg/mL).Khusnul FadhilahSubagus WahyuonoPuji AstutiMBI & UNS Soloarticlecytotoxict47dhepg2lansium domesticumBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cytotoxic t47d hepg2 lansium domesticum Biology (General) QH301-705.5
spellingShingle	cytotoxic t47d hepg2 lansium domesticum Biology (General) QH301-705.5 Khusnul Fadhilah Subagus Wahyuono Puji Astuti Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines
description	Abstract. Fadhilah K, Wahyuono S, Astuti P. 2021. Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines. Biodiversitas 22: 3743-3748. Lansium domesticum (Fam. Meliaceae), a tropical fruit (local name, Duku), has been reported to have various biological activities. At the moment we emphasize on searching compounds to have cytotoxic activity from the peel of L. domesticum. The study was initiated by extraction with EtOAc followed by fractionation that was monitored by thin-layer chromatography (TLC) and cytotoxicity against T-47D and HepG2 cells. The EtOAc extract of the sample was triturated with n-hexane to give n-hexane soluble (A) n-hexane insoluble fractions (B). The B fraction was fractionated by vacuum column chromatography using gradient solvent composition of n-hexane: acetone to give 18 fractions. According to TLC similarity pictures, fractions were combined to give 6 fractions (I-VI). Isolation was performed using preparative TLC, and the cytotoxic assay was performed using MTT method. The isolated compound was identified as a sesquiterpene having one aldehyde functional group, based on spectroscopic data.The isolated compound displayed cytotoxic activity on T-47D (IC50, 48.58 + 0.96 µg/mL) and HepG2 (IC50, 127.45 + 25.76 µg/mL).
format	article
author	Khusnul Fadhilah Subagus Wahyuono Puji Astuti
author_facet	Khusnul Fadhilah Subagus Wahyuono Puji Astuti
author_sort	Khusnul Fadhilah
title	Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines
title_short	Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines
title_full	Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines
title_fullStr	Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines
title_full_unstemmed	Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines
title_sort	fractions and isolated compounds from lansium domesticum fruit peel exhibited cytotoxic activity against t-47d and hepg2 cell lines
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/a183014bc2ce4e4e8e88d142fbdb443d
work_keys_str_mv	AT khusnulfadhilah fractionsandisolatedcompoundsfromlansiumdomesticumfruitpeelexhibitedcytotoxicactivityagainstt47dandhepg2celllines AT subaguswahyuono fractionsandisolatedcompoundsfromlansiumdomesticumfruitpeelexhibitedcytotoxicactivityagainstt47dandhepg2celllines AT pujiastuti fractionsandisolatedcompoundsfromlansiumdomesticumfruitpeelexhibitedcytotoxicactivityagainstt47dandhepg2celllines
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Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines

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