Action mode of ursolic acid as a natural antioxidant and inhibitor of superoxide dismutase: In vitro and in silico study

Recently, the antioxidant is applied for the teeth bleaching treatment as an alternative of toxic material of hydrogen peroxide that is used in teeth bleaching. One of natural sources antioxidant is Uncaria gambir those containing active antioxidant agents. To be applied as a new bioactive constitue...

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Autores principales:	Ara Deani Somantri, Dikdik Kurnia, Achmad Zainuddin, Hendra Dian Adhita Dharsono, Mieke Hemiawati Satari
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2021
Materias:	dpph superoxide dismutase enzyme uncaria gambir ursolic acid Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/a1850666768d4db8bd1b5204b4e56903
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spelling	oai:doaj.org-article:a1850666768d4db8bd1b5204b4e569032021-11-12T10:17:53ZAction mode of ursolic acid as a natural antioxidant and inhibitor of superoxide dismutase: In vitro and in silico study2231-40400976-209410.4103/japtr.japtr_90_21https://doaj.org/article/a1850666768d4db8bd1b5204b4e569032021-01-01T00:00:00Zhttp://www.japtr.org/article.asp?issn=2231-4040;year=2021;volume=12;issue=4;spage=389;epage=394;aulast=Somantrihttps://doaj.org/toc/2231-4040https://doaj.org/toc/0976-2094Recently, the antioxidant is applied for the teeth bleaching treatment as an alternative of toxic material of hydrogen peroxide that is used in teeth bleaching. One of natural sources antioxidant is Uncaria gambir those containing active antioxidant agents. To be applied as a new bioactive constituent in teeth bleaching treatment, a preexperimental study is performed. The aim of the study is to identify the antioxidant constituent of U. gambir and predict their activity including action mode as an inhibitor of enzyme superoxide dismutase (SOD) through in vitro and in silico method. Combination of chromatography methods and spectroscopic analysis is used for isolated bioactive antioxidant constituent. The antioxidant activity was evaluated by in vitro assay against diphenylpicrylhydrazyl (DPPH) and SOD, respectively, while prediction of action mode of the active compounds as SOD-mutant enzyme inhibitor was conducted by in silico study using AutoDock 4.2 program. Antioxidant of ursolic acid was isolated from U. gambir with inhibitory concentration50 values 1721 ± 30.6 and 392 ± 53.57 μg/mL, respectively, against DPPH and SOD. By in silico study presented that ursolic acid inhibited SOD enzyme with a binding affinity of − 5.4 kcal/mol those higher than a quercetin as a positive control. The ursolic acid was identified as a potential natural antioxidant with potentially activity to inhibit SOD mutant.Ara Deani SomantriDikdik KurniaAchmad ZainuddinHendra Dian Adhita DharsonoMieke Hemiawati SatariWolters Kluwer Medknow Publicationsarticledpphsuperoxide dismutase enzyme uncaria gambir ursolic acidTherapeutics. PharmacologyRM1-950Pharmacy and materia medicaRS1-441ENJournal of Advanced Pharmaceutical Technology & Research, Vol 12, Iss 4, Pp 389-394 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dpph superoxide dismutase enzyme uncaria gambir ursolic acid Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441
spellingShingle	dpph superoxide dismutase enzyme uncaria gambir ursolic acid Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441 Ara Deani Somantri Dikdik Kurnia Achmad Zainuddin Hendra Dian Adhita Dharsono Mieke Hemiawati Satari Action mode of ursolic acid as a natural antioxidant and inhibitor of superoxide dismutase: In vitro and in silico study
description	Recently, the antioxidant is applied for the teeth bleaching treatment as an alternative of toxic material of hydrogen peroxide that is used in teeth bleaching. One of natural sources antioxidant is Uncaria gambir those containing active antioxidant agents. To be applied as a new bioactive constituent in teeth bleaching treatment, a preexperimental study is performed. The aim of the study is to identify the antioxidant constituent of U. gambir and predict their activity including action mode as an inhibitor of enzyme superoxide dismutase (SOD) through in vitro and in silico method. Combination of chromatography methods and spectroscopic analysis is used for isolated bioactive antioxidant constituent. The antioxidant activity was evaluated by in vitro assay against diphenylpicrylhydrazyl (DPPH) and SOD, respectively, while prediction of action mode of the active compounds as SOD-mutant enzyme inhibitor was conducted by in silico study using AutoDock 4.2 program. Antioxidant of ursolic acid was isolated from U. gambir with inhibitory concentration50 values 1721 ± 30.6 and 392 ± 53.57 μg/mL, respectively, against DPPH and SOD. By in silico study presented that ursolic acid inhibited SOD enzyme with a binding affinity of − 5.4 kcal/mol those higher than a quercetin as a positive control. The ursolic acid was identified as a potential natural antioxidant with potentially activity to inhibit SOD mutant.
format	article
author	Ara Deani Somantri Dikdik Kurnia Achmad Zainuddin Hendra Dian Adhita Dharsono Mieke Hemiawati Satari
author_facet	Ara Deani Somantri Dikdik Kurnia Achmad Zainuddin Hendra Dian Adhita Dharsono Mieke Hemiawati Satari
author_sort	Ara Deani Somantri
title	Action mode of ursolic acid as a natural antioxidant and inhibitor of superoxide dismutase: In vitro and in silico study
title_short	Action mode of ursolic acid as a natural antioxidant and inhibitor of superoxide dismutase: In vitro and in silico study
title_full	Action mode of ursolic acid as a natural antioxidant and inhibitor of superoxide dismutase: In vitro and in silico study
title_fullStr	Action mode of ursolic acid as a natural antioxidant and inhibitor of superoxide dismutase: In vitro and in silico study
title_full_unstemmed	Action mode of ursolic acid as a natural antioxidant and inhibitor of superoxide dismutase: In vitro and in silico study
title_sort	action mode of ursolic acid as a natural antioxidant and inhibitor of superoxide dismutase: in vitro and in silico study
publisher	Wolters Kluwer Medknow Publications
publishDate	2021
url	https://doaj.org/article/a1850666768d4db8bd1b5204b4e56903
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Action mode of ursolic acid as a natural antioxidant and inhibitor of superoxide dismutase: In vitro and in silico study

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