The Wnt receptor Ryk is a negative regulator of mammalian dendrite morphogenesis

Abstract The unique dendritic architecture of a given neuronal subtype determines its synaptic connectivity and ability to integrate into functional neuronal networks. It is now clear that abnormal dendritic structure is associated with neuropsychiatric and neurodegenerative disorders. Currently, ho...

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Autores principales:	Vanessa Lanoue, Michael Langford, Amanda White, Kai Sempert, Lily Fogg, Helen M. Cooper
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a1859b9f6ea446528bbd49524c971c62
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spelling	oai:doaj.org-article:a1859b9f6ea446528bbd49524c971c622021-12-02T11:41:20ZThe Wnt receptor Ryk is a negative regulator of mammalian dendrite morphogenesis10.1038/s41598-017-06140-z2045-2322https://doaj.org/article/a1859b9f6ea446528bbd49524c971c622017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06140-zhttps://doaj.org/toc/2045-2322Abstract The unique dendritic architecture of a given neuronal subtype determines its synaptic connectivity and ability to integrate into functional neuronal networks. It is now clear that abnormal dendritic structure is associated with neuropsychiatric and neurodegenerative disorders. Currently, however, the nature of the extrinsic factors that limit dendritic growth and branching within predetermined boundaries in the mammalian brain is poorly understood. Here we identify the Wnt receptor Ryk as a novel negative regulator of dendritic arborisation. We demonstrate that loss of Ryk in mouse hippocampal and cortical neurons promotes excessive dendrite growth and branching in vitro. Conversely, overexpression of wildtype Ryk restricts these processes, confirming that Ryk acts to restrain dendrite arborisation. Furthermore, we identify a hitherto uncharacterized membrane proximal subdomain crucial for Ryk-mediated suppression of dendrite morphogenesis, suggesting that it may act through a novel signalling pathway to constrain dendrite complexity. We also demonstrate that Ryk performs a similar function in vivo as Ryk haploinsufficient postnatal animals exhibit excessive dendrite growth and branching in layer 2/3 pyramidal neurons of the somatosensory cortex. These findings reveal an essential role for Ryk in regulating dendrite complexity and raise the intriguing possibility that it may influence neural plasticity by modifying dendritic structure.Vanessa LanoueMichael LangfordAmanda WhiteKai SempertLily FoggHelen M. CooperNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vanessa Lanoue Michael Langford Amanda White Kai Sempert Lily Fogg Helen M. Cooper The Wnt receptor Ryk is a negative regulator of mammalian dendrite morphogenesis
description	Abstract The unique dendritic architecture of a given neuronal subtype determines its synaptic connectivity and ability to integrate into functional neuronal networks. It is now clear that abnormal dendritic structure is associated with neuropsychiatric and neurodegenerative disorders. Currently, however, the nature of the extrinsic factors that limit dendritic growth and branching within predetermined boundaries in the mammalian brain is poorly understood. Here we identify the Wnt receptor Ryk as a novel negative regulator of dendritic arborisation. We demonstrate that loss of Ryk in mouse hippocampal and cortical neurons promotes excessive dendrite growth and branching in vitro. Conversely, overexpression of wildtype Ryk restricts these processes, confirming that Ryk acts to restrain dendrite arborisation. Furthermore, we identify a hitherto uncharacterized membrane proximal subdomain crucial for Ryk-mediated suppression of dendrite morphogenesis, suggesting that it may act through a novel signalling pathway to constrain dendrite complexity. We also demonstrate that Ryk performs a similar function in vivo as Ryk haploinsufficient postnatal animals exhibit excessive dendrite growth and branching in layer 2/3 pyramidal neurons of the somatosensory cortex. These findings reveal an essential role for Ryk in regulating dendrite complexity and raise the intriguing possibility that it may influence neural plasticity by modifying dendritic structure.
format	article
author	Vanessa Lanoue Michael Langford Amanda White Kai Sempert Lily Fogg Helen M. Cooper
author_facet	Vanessa Lanoue Michael Langford Amanda White Kai Sempert Lily Fogg Helen M. Cooper
author_sort	Vanessa Lanoue
title	The Wnt receptor Ryk is a negative regulator of mammalian dendrite morphogenesis
title_short	The Wnt receptor Ryk is a negative regulator of mammalian dendrite morphogenesis
title_full	The Wnt receptor Ryk is a negative regulator of mammalian dendrite morphogenesis
title_fullStr	The Wnt receptor Ryk is a negative regulator of mammalian dendrite morphogenesis
title_full_unstemmed	The Wnt receptor Ryk is a negative regulator of mammalian dendrite morphogenesis
title_sort	wnt receptor ryk is a negative regulator of mammalian dendrite morphogenesis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a1859b9f6ea446528bbd49524c971c62
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The Wnt receptor Ryk is a negative regulator of mammalian dendrite morphogenesis

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