Spatial distribution of epigenetic modifications in Brachypodium distachyon embryos during seed maturation and germination.

Seed development involves a plethora of spatially and temporally synchronised genetic and epigenetic processes. Although it has been shown that epigenetic mechanisms, such as DNA methylation and chromatin remodelling, act on a large number of genes during seed development and germination, to date th...

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Autores principales:	Elzbieta Wolny, Agnieszka Braszewska-Zalewska, Robert Hasterok
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a187093ff63e49d7aa42bff0c759a5d3
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spelling	oai:doaj.org-article:a187093ff63e49d7aa42bff0c759a5d32021-11-25T06:09:15ZSpatial distribution of epigenetic modifications in Brachypodium distachyon embryos during seed maturation and germination.1932-620310.1371/journal.pone.0101246https://doaj.org/article/a187093ff63e49d7aa42bff0c759a5d32014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25006668/?tool=EBIhttps://doaj.org/toc/1932-6203Seed development involves a plethora of spatially and temporally synchronised genetic and epigenetic processes. Although it has been shown that epigenetic mechanisms, such as DNA methylation and chromatin remodelling, act on a large number of genes during seed development and germination, to date the global levels of histone modifications have not been studied in a tissue-specific manner in plant embryos. In this study we analysed the distribution of three epigenetic markers, i.e. H4K5ac, H3K4me2 and H3K4me1 in 'matured', 'dry' and 'germinating' embryos of a model grass, Brachypodium distachyon (Brachypodium). Our results indicate that the abundance of these modifications differs considerably in various organs and tissues of the three types of Brachypodium embryos. Embryos from matured seeds were characterised by the highest level of H4K5ac in RAM and epithelial cells of the scutellum, whereas this modification was not observed in the coleorhiza. In this type of embryos H3K4me2 was most evident in epithelial cells of the scutellum. In 'dry' embryos H4K5ac was highest in the coleorhiza but was not present in the nuclei of the scutellum. H3K4me1 was the most elevated in the coleoptile but absent from the coleorhiza, whereas H3K4me2 was the most prominent in leaf primordia and RAM. In embryos from germinating seeds H4K5ac was the most evident in the scutellum but not present in the coleoptile, similarly H3K4me1 was the highest in the scutellum and very low in the coleoptile, while the highest level of H3K4me2 was observed in the coleoptile and the lowest in the coleorhiza. The distinct patterns of epigenetic modifications that were observed may be involved in the switch of the gene expression profiles in specific organs of the developing embryo and may be linked with the physiological changes that accompany seed desiccation, imbibition and germination.Elzbieta WolnyAgnieszka Braszewska-ZalewskaRobert HasterokPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e101246 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elzbieta Wolny Agnieszka Braszewska-Zalewska Robert Hasterok Spatial distribution of epigenetic modifications in Brachypodium distachyon embryos during seed maturation and germination.
description	Seed development involves a plethora of spatially and temporally synchronised genetic and epigenetic processes. Although it has been shown that epigenetic mechanisms, such as DNA methylation and chromatin remodelling, act on a large number of genes during seed development and germination, to date the global levels of histone modifications have not been studied in a tissue-specific manner in plant embryos. In this study we analysed the distribution of three epigenetic markers, i.e. H4K5ac, H3K4me2 and H3K4me1 in 'matured', 'dry' and 'germinating' embryos of a model grass, Brachypodium distachyon (Brachypodium). Our results indicate that the abundance of these modifications differs considerably in various organs and tissues of the three types of Brachypodium embryos. Embryos from matured seeds were characterised by the highest level of H4K5ac in RAM and epithelial cells of the scutellum, whereas this modification was not observed in the coleorhiza. In this type of embryos H3K4me2 was most evident in epithelial cells of the scutellum. In 'dry' embryos H4K5ac was highest in the coleorhiza but was not present in the nuclei of the scutellum. H3K4me1 was the most elevated in the coleoptile but absent from the coleorhiza, whereas H3K4me2 was the most prominent in leaf primordia and RAM. In embryos from germinating seeds H4K5ac was the most evident in the scutellum but not present in the coleoptile, similarly H3K4me1 was the highest in the scutellum and very low in the coleoptile, while the highest level of H3K4me2 was observed in the coleoptile and the lowest in the coleorhiza. The distinct patterns of epigenetic modifications that were observed may be involved in the switch of the gene expression profiles in specific organs of the developing embryo and may be linked with the physiological changes that accompany seed desiccation, imbibition and germination.
format	article
author	Elzbieta Wolny Agnieszka Braszewska-Zalewska Robert Hasterok
author_facet	Elzbieta Wolny Agnieszka Braszewska-Zalewska Robert Hasterok
author_sort	Elzbieta Wolny
title	Spatial distribution of epigenetic modifications in Brachypodium distachyon embryos during seed maturation and germination.
title_short	Spatial distribution of epigenetic modifications in Brachypodium distachyon embryos during seed maturation and germination.
title_full	Spatial distribution of epigenetic modifications in Brachypodium distachyon embryos during seed maturation and germination.
title_fullStr	Spatial distribution of epigenetic modifications in Brachypodium distachyon embryos during seed maturation and germination.
title_full_unstemmed	Spatial distribution of epigenetic modifications in Brachypodium distachyon embryos during seed maturation and germination.
title_sort	spatial distribution of epigenetic modifications in brachypodium distachyon embryos during seed maturation and germination.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/a187093ff63e49d7aa42bff0c759a5d3
work_keys_str_mv	AT elzbietawolny spatialdistributionofepigeneticmodificationsinbrachypodiumdistachyonembryosduringseedmaturationandgermination AT agnieszkabraszewskazalewska spatialdistributionofepigeneticmodificationsinbrachypodiumdistachyonembryosduringseedmaturationandgermination AT roberthasterok spatialdistributionofepigeneticmodificationsinbrachypodiumdistachyonembryosduringseedmaturationandgermination
_version_	1718414120633499648

Spatial distribution of epigenetic modifications in Brachypodium distachyon embryos during seed maturation and germination.

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