Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners

Abstract The collection and presentation of accurate reproductive data from wild fish has historically been somewhat problematic, especially for serially spawning species. Therefore, the aim of the current study was to develop a novel method of assessing female spawning status that is robust to vari...

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Autores principales:	Kelli C. Anderson, Maud Alix, Katerina Charitonidou, Anders Thorsen, Grethe Thorsheim, Kostas Ganias, Thassya C. dos Santos Schmidt, Olav Sigurd Kjesbu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a18968bde94842b5801b4029aa518d1b
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spelling	oai:doaj.org-article:a18968bde94842b5801b4029aa518d1b2021-12-02T17:40:44ZDevelopment of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners10.1038/s41598-020-66601-w2045-2322https://doaj.org/article/a18968bde94842b5801b4029aa518d1b2020-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-66601-whttps://doaj.org/toc/2045-2322Abstract The collection and presentation of accurate reproductive data from wild fish has historically been somewhat problematic, especially for serially spawning species. Therefore, the aim of the current study was to develop a novel method of assessing female spawning status that is robust to variation in oocyte dynamics between specimens. Atlantic cod (Barents Sea stock) were used to develop the new ‘ultrametric’ method, that is based on the progressive depletion of the vitellogenic oocyte pool relative to the rather constant previtellogenic oocyte (PVO) pool. Fish were subsequently partitioned into one of four categories that accurately reflected changes in their oocyte size frequency distribution characteristics and gonadosomatic index throughout spawning. The ultrametric method overcomes difficulties associated with presence of bimodal oocyte distributions, oocyte tails, lack of clear hiatus region, and presence of free ova, and can be implemented at a single sampling point. Much of the workflow is fully automated, and the technique may circumvent the need for histological analysis depending on the desired outcome. The ultrametric method differs from the traditional autodiametric method in that PVOs can be separated by ultrasonication and then enumerated, and ovarian homogeneity is not a mandatory requirement per se. The method is designed for determinate spawners but might be extended to include indeterminate spawners.Kelli C. AndersonMaud AlixKaterina CharitonidouAnders ThorsenGrethe ThorsheimKostas GaniasThassya C. dos Santos SchmidtOlav Sigurd KjesbuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kelli C. Anderson Maud Alix Katerina Charitonidou Anders Thorsen Grethe Thorsheim Kostas Ganias Thassya C. dos Santos Schmidt Olav Sigurd Kjesbu Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners
description	Abstract The collection and presentation of accurate reproductive data from wild fish has historically been somewhat problematic, especially for serially spawning species. Therefore, the aim of the current study was to develop a novel method of assessing female spawning status that is robust to variation in oocyte dynamics between specimens. Atlantic cod (Barents Sea stock) were used to develop the new ‘ultrametric’ method, that is based on the progressive depletion of the vitellogenic oocyte pool relative to the rather constant previtellogenic oocyte (PVO) pool. Fish were subsequently partitioned into one of four categories that accurately reflected changes in their oocyte size frequency distribution characteristics and gonadosomatic index throughout spawning. The ultrametric method overcomes difficulties associated with presence of bimodal oocyte distributions, oocyte tails, lack of clear hiatus region, and presence of free ova, and can be implemented at a single sampling point. Much of the workflow is fully automated, and the technique may circumvent the need for histological analysis depending on the desired outcome. The ultrametric method differs from the traditional autodiametric method in that PVOs can be separated by ultrasonication and then enumerated, and ovarian homogeneity is not a mandatory requirement per se. The method is designed for determinate spawners but might be extended to include indeterminate spawners.
format	article
author	Kelli C. Anderson Maud Alix Katerina Charitonidou Anders Thorsen Grethe Thorsheim Kostas Ganias Thassya C. dos Santos Schmidt Olav Sigurd Kjesbu
author_facet	Kelli C. Anderson Maud Alix Katerina Charitonidou Anders Thorsen Grethe Thorsheim Kostas Ganias Thassya C. dos Santos Schmidt Olav Sigurd Kjesbu
author_sort	Kelli C. Anderson
title	Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners
title_short	Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners
title_full	Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners
title_fullStr	Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners
title_full_unstemmed	Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners
title_sort	development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/a18968bde94842b5801b4029aa518d1b
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Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners

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