The m6A RNA Modification Modulates Gene Expression and Fibrosis-Related Pathways in Hypertrophic Scar

Purpose: To systematically analyze the overall m6A modification pattern in hyperplastic scars (HS).Methods: The m6A modification patterns in HS and normal skin (NS) tissues were described by m6A sequencing and RNA sequencing, and subsequently bioinformatics analysis was performed. The m6A-related RN...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Si-Yu Liu, Jun-Jie Wu, Zhong-hua Chen, Ming-Li Zou, Ying-ying Teng, Kai-Wen Zhang, Yue-Yue Li, Dang-yang Guo, Feng-Lai Yuan
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	hypertrophic scar m6A sequencing modification patterns N6-methyladenosine MeRIP-seq Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a18989ec44294e9f853a06156de9db29
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a18989ec44294e9f853a06156de9db29
record_format	dspace
spelling	oai:doaj.org-article:a18989ec44294e9f853a06156de9db292021-11-15T06:57:16ZThe m6A RNA Modification Modulates Gene Expression and Fibrosis-Related Pathways in Hypertrophic Scar2296-634X10.3389/fcell.2021.748703https://doaj.org/article/a18989ec44294e9f853a06156de9db292021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.748703/fullhttps://doaj.org/toc/2296-634XPurpose: To systematically analyze the overall m6A modification pattern in hyperplastic scars (HS).Methods: The m6A modification patterns in HS and normal skin (NS) tissues were described by m6A sequencing and RNA sequencing, and subsequently bioinformatics analysis was performed. The m6A-related RNA was immunoprecipitated and verified by real-time quantitative PCR.Results: The appearance of 14,791 new m6A peaks in the HS sample was accompanied by the disappearance of 7,835 peaks. The unique m6A-related genes in HS were thus associated with fibrosis-related pathways. We identified the differentially expressed mRNA transcripts in HS samples with hyper-methylated or hypo-methylated m6A peaks.Conclusion: This study is the first to map the m6A transcriptome of human HS, which may help clarify the possible mechanism of m6A-mediated gene expression regulation.Si-Yu LiuJun-Jie WuZhong-hua ChenMing-Li ZouYing-ying TengKai-Wen ZhangYue-Yue LiDang-yang GuoFeng-Lai YuanFeng-Lai YuanFeng-Lai YuanFrontiers Media S.A.articlehypertrophic scarm6A sequencingmodification patternsN6-methyladenosineMeRIP-seqBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hypertrophic scar m6A sequencing modification patterns N6-methyladenosine MeRIP-seq Biology (General) QH301-705.5
spellingShingle	hypertrophic scar m6A sequencing modification patterns N6-methyladenosine MeRIP-seq Biology (General) QH301-705.5 Si-Yu Liu Jun-Jie Wu Zhong-hua Chen Ming-Li Zou Ying-ying Teng Kai-Wen Zhang Yue-Yue Li Dang-yang Guo Feng-Lai Yuan Feng-Lai Yuan Feng-Lai Yuan The m6A RNA Modification Modulates Gene Expression and Fibrosis-Related Pathways in Hypertrophic Scar
description	Purpose: To systematically analyze the overall m6A modification pattern in hyperplastic scars (HS).Methods: The m6A modification patterns in HS and normal skin (NS) tissues were described by m6A sequencing and RNA sequencing, and subsequently bioinformatics analysis was performed. The m6A-related RNA was immunoprecipitated and verified by real-time quantitative PCR.Results: The appearance of 14,791 new m6A peaks in the HS sample was accompanied by the disappearance of 7,835 peaks. The unique m6A-related genes in HS were thus associated with fibrosis-related pathways. We identified the differentially expressed mRNA transcripts in HS samples with hyper-methylated or hypo-methylated m6A peaks.Conclusion: This study is the first to map the m6A transcriptome of human HS, which may help clarify the possible mechanism of m6A-mediated gene expression regulation.
format	article
author	Si-Yu Liu Jun-Jie Wu Zhong-hua Chen Ming-Li Zou Ying-ying Teng Kai-Wen Zhang Yue-Yue Li Dang-yang Guo Feng-Lai Yuan Feng-Lai Yuan Feng-Lai Yuan
author_facet	Si-Yu Liu Jun-Jie Wu Zhong-hua Chen Ming-Li Zou Ying-ying Teng Kai-Wen Zhang Yue-Yue Li Dang-yang Guo Feng-Lai Yuan Feng-Lai Yuan Feng-Lai Yuan
author_sort	Si-Yu Liu
title	The m6A RNA Modification Modulates Gene Expression and Fibrosis-Related Pathways in Hypertrophic Scar
title_short	The m6A RNA Modification Modulates Gene Expression and Fibrosis-Related Pathways in Hypertrophic Scar
title_full	The m6A RNA Modification Modulates Gene Expression and Fibrosis-Related Pathways in Hypertrophic Scar
title_fullStr	The m6A RNA Modification Modulates Gene Expression and Fibrosis-Related Pathways in Hypertrophic Scar
title_full_unstemmed	The m6A RNA Modification Modulates Gene Expression and Fibrosis-Related Pathways in Hypertrophic Scar
title_sort	m6a rna modification modulates gene expression and fibrosis-related pathways in hypertrophic scar
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a18989ec44294e9f853a06156de9db29
work_keys_str_mv	AT siyuliu them6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT junjiewu them6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT zhonghuachen them6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT minglizou them6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT yingyingteng them6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT kaiwenzhang them6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT yueyueli them6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT dangyangguo them6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT fenglaiyuan them6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT fenglaiyuan them6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT fenglaiyuan them6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT siyuliu m6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT junjiewu m6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT zhonghuachen m6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT minglizou m6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT yingyingteng m6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT kaiwenzhang m6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT yueyueli m6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT dangyangguo m6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT fenglaiyuan m6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT fenglaiyuan m6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar AT fenglaiyuan m6arnamodificationmodulatesgeneexpressionandfibrosisrelatedpathwaysinhypertrophicscar
_version_	1718428513167474688

The m6A RNA Modification Modulates Gene Expression and Fibrosis-Related Pathways in Hypertrophic Scar

Ejemplares similares