Endothelial colony forming cells and mesenchymal progenitor cells form blood vessels and increase blood flow in ischemic muscle

Abstract Here we investigated whether endothelial colony forming cells (ECFC) and mesenchymal progenitor cells (MPC) form vascular networks and restore blood flow in ischemic skeletal muscle, and whether host myeloid cells play a role. ECFC + MPC, ECFC alone, MPC alone, or vehicle alone were injecte...

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Autores principales:	Kyu-Tae Kang, Ruei-Zeng Lin, David Kuppermann, Juan M. Melero-Martin, Joyce Bischoff
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a18ab1cf2b7e4ac8a74dcb2f6a42d991
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spelling	oai:doaj.org-article:a18ab1cf2b7e4ac8a74dcb2f6a42d9912021-12-02T16:06:31ZEndothelial colony forming cells and mesenchymal progenitor cells form blood vessels and increase blood flow in ischemic muscle10.1038/s41598-017-00809-12045-2322https://doaj.org/article/a18ab1cf2b7e4ac8a74dcb2f6a42d9912017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00809-1https://doaj.org/toc/2045-2322Abstract Here we investigated whether endothelial colony forming cells (ECFC) and mesenchymal progenitor cells (MPC) form vascular networks and restore blood flow in ischemic skeletal muscle, and whether host myeloid cells play a role. ECFC + MPC, ECFC alone, MPC alone, or vehicle alone were injected into the hind limb ischemic muscle one day after ligation of femoral artery and vein. At day 5, hind limbs injected with ECFC + MPC showed greater blood flow recovery compared with ECFC, MPC, or vehicle. Tail vein injection of human endothelial specific Ulex europaeus agglutinin-I demonstrated an increased number of perfused human vessels in ECFC + MPC compared with ECFC. In vivo bioluminescence imaging showed ECFC persisted for 14 days in ECFC + MPC-injected hind limbs. Flow cytometric analysis of ischemic muscles at day 2 revealed increased myeloid lineage cells in ECFC + MPC-injected muscles compared to vehicle-injected muscles. Neutrophils declined by day 7, while the number of myeloid cells, macrophages, and monocytes did not. Systemic myeloid cell depletion with anti-Gr-1 antibody blocked the improved blood flow observed with ECFC + MPC and reduced ECFC and MPC retention. Our data suggest that ECFC + MPC delivery could be used to reestablish blood flow in ischemic tissues, and this may be enhanced by coordinated recruitment of host myeloid cells.Kyu-Tae KangRuei-Zeng LinDavid KuppermannJuan M. Melero-MartinJoyce BischoffNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kyu-Tae Kang Ruei-Zeng Lin David Kuppermann Juan M. Melero-Martin Joyce Bischoff Endothelial colony forming cells and mesenchymal progenitor cells form blood vessels and increase blood flow in ischemic muscle
description	Abstract Here we investigated whether endothelial colony forming cells (ECFC) and mesenchymal progenitor cells (MPC) form vascular networks and restore blood flow in ischemic skeletal muscle, and whether host myeloid cells play a role. ECFC + MPC, ECFC alone, MPC alone, or vehicle alone were injected into the hind limb ischemic muscle one day after ligation of femoral artery and vein. At day 5, hind limbs injected with ECFC + MPC showed greater blood flow recovery compared with ECFC, MPC, or vehicle. Tail vein injection of human endothelial specific Ulex europaeus agglutinin-I demonstrated an increased number of perfused human vessels in ECFC + MPC compared with ECFC. In vivo bioluminescence imaging showed ECFC persisted for 14 days in ECFC + MPC-injected hind limbs. Flow cytometric analysis of ischemic muscles at day 2 revealed increased myeloid lineage cells in ECFC + MPC-injected muscles compared to vehicle-injected muscles. Neutrophils declined by day 7, while the number of myeloid cells, macrophages, and monocytes did not. Systemic myeloid cell depletion with anti-Gr-1 antibody blocked the improved blood flow observed with ECFC + MPC and reduced ECFC and MPC retention. Our data suggest that ECFC + MPC delivery could be used to reestablish blood flow in ischemic tissues, and this may be enhanced by coordinated recruitment of host myeloid cells.
format	article
author	Kyu-Tae Kang Ruei-Zeng Lin David Kuppermann Juan M. Melero-Martin Joyce Bischoff
author_facet	Kyu-Tae Kang Ruei-Zeng Lin David Kuppermann Juan M. Melero-Martin Joyce Bischoff
author_sort	Kyu-Tae Kang
title	Endothelial colony forming cells and mesenchymal progenitor cells form blood vessels and increase blood flow in ischemic muscle
title_short	Endothelial colony forming cells and mesenchymal progenitor cells form blood vessels and increase blood flow in ischemic muscle
title_full	Endothelial colony forming cells and mesenchymal progenitor cells form blood vessels and increase blood flow in ischemic muscle
title_fullStr	Endothelial colony forming cells and mesenchymal progenitor cells form blood vessels and increase blood flow in ischemic muscle
title_full_unstemmed	Endothelial colony forming cells and mesenchymal progenitor cells form blood vessels and increase blood flow in ischemic muscle
title_sort	endothelial colony forming cells and mesenchymal progenitor cells form blood vessels and increase blood flow in ischemic muscle
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a18ab1cf2b7e4ac8a74dcb2f6a42d991
work_keys_str_mv	AT kyutaekang endothelialcolonyformingcellsandmesenchymalprogenitorcellsformbloodvesselsandincreasebloodflowinischemicmuscle AT rueizenglin endothelialcolonyformingcellsandmesenchymalprogenitorcellsformbloodvesselsandincreasebloodflowinischemicmuscle AT davidkuppermann endothelialcolonyformingcellsandmesenchymalprogenitorcellsformbloodvesselsandincreasebloodflowinischemicmuscle AT juanmmeleromartin endothelialcolonyformingcellsandmesenchymalprogenitorcellsformbloodvesselsandincreasebloodflowinischemicmuscle AT joycebischoff endothelialcolonyformingcellsandmesenchymalprogenitorcellsformbloodvesselsandincreasebloodflowinischemicmuscle
_version_	1718384979255230464

Endothelial colony forming cells and mesenchymal progenitor cells form blood vessels and increase blood flow in ischemic muscle

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