Antibiotic resistance in a predominantly occurring Gram-negative bacterial community from treated sewage to assess the need for going beyond coliform standards

Antibiotic resistance surveillance is an objective of global action plan on antimicrobial resistance endorsed by the World Health Organization. The current study reports the identification of frequently occurring Gram-negative bacterial community (GNBC) previously isolated from municipal treated was...

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Autores principales: Sandeep Singh Shekhawat, Niha Mohan Kulshreshtha, Rinki Mishra, Sudipti Arora, Vivekanand Vivekanand, Akhilendra Bhushan Gupta
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Publicado: IWA Publishing 2021
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Acceso en línea:https://doaj.org/article/a18d3aab12184c35adacd9481186c124
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spelling oai:doaj.org-article:a18d3aab12184c35adacd9481186c1242021-11-06T03:51:16ZAntibiotic resistance in a predominantly occurring Gram-negative bacterial community from treated sewage to assess the need for going beyond coliform standards2709-80442709-805210.2166/wqrj.2021.001https://doaj.org/article/a18d3aab12184c35adacd9481186c1242021-08-01T00:00:00Zhttp://wqrjc.iwaponline.com/content/56/3/143https://doaj.org/toc/2709-8044https://doaj.org/toc/2709-8052Antibiotic resistance surveillance is an objective of global action plan on antimicrobial resistance endorsed by the World Health Organization. The current study reports the identification of frequently occurring Gram-negative bacterial community (GNBC) previously isolated from municipal treated wastewater and their antibiotic resistance profiles. Further, the genes responsible for extended-spectrum beta lactamases (ESBL) activity were identified in ESBL-positive organisms. The isolates were characterized using biochemical assays and identification was confirmed by VITEK®2 automated system. Antibiotic susceptibility testing against seven different classes of antibiotics was also performed on the same system using AST-N280 cards. The most dominant isolates identified were Acinetobacter baumannii, Morganella morganii, Kluyvera intermedia, Stenotrophomonas maltophilia, Escherichia coli, Aeromonas hydrophila/caviae, Klebsiella pneumoniae, Enterobacter cloacae and Citrobacter freundii. The isolates were observed to be significantly resistant against the antibiotics amoxicillin-clavulanic acid, cefuroxime, cefuroxime axetil and colistin. Two of the isolates, E. cloacae sp. dissolvens and S. maltophilia, were found to be positive for ESBL activity encoded by blaCTX-M gene. The possible intrusion of hospital wastewater in domestic sewage is also discussed. This study may help assess the risk of wastewater reuse by detecting dominant bacteria as a step towards the development of new microbiological standards. HIGHLIGHTS Stenotrophomonas maltophilia found to be most resistant and E. coli least resistant among the isolates.; Five isolates found to be resistant to the last resort antibiotic Colistin.; Molecular determinant of two extended spectrum beta lactamase producing isolates found to be blaCTX-M gene.; This report might aid in devising a suitable strategy for reducing risk to human health upon treated wastewater reuse.;Sandeep Singh ShekhawatNiha Mohan KulshreshthaRinki MishraSudipti AroraVivekanand VivekanandAkhilendra Bhushan GuptaIWA Publishingarticleesblgram-negative bacterial communitymultiple drug resistancetreated municipal sewagevitek®2Environmental technology. Sanitary engineeringTD1-1066ENWater Quality Research Journal, Vol 56, Iss 3, Pp 143-154 (2021)
institution DOAJ
collection DOAJ
language EN
topic esbl
gram-negative bacterial community
multiple drug resistance
treated municipal sewage
vitek®2
Environmental technology. Sanitary engineering
TD1-1066
spellingShingle esbl
gram-negative bacterial community
multiple drug resistance
treated municipal sewage
vitek®2
Environmental technology. Sanitary engineering
TD1-1066
Sandeep Singh Shekhawat
Niha Mohan Kulshreshtha
Rinki Mishra
Sudipti Arora
Vivekanand Vivekanand
Akhilendra Bhushan Gupta
Antibiotic resistance in a predominantly occurring Gram-negative bacterial community from treated sewage to assess the need for going beyond coliform standards
description Antibiotic resistance surveillance is an objective of global action plan on antimicrobial resistance endorsed by the World Health Organization. The current study reports the identification of frequently occurring Gram-negative bacterial community (GNBC) previously isolated from municipal treated wastewater and their antibiotic resistance profiles. Further, the genes responsible for extended-spectrum beta lactamases (ESBL) activity were identified in ESBL-positive organisms. The isolates were characterized using biochemical assays and identification was confirmed by VITEK®2 automated system. Antibiotic susceptibility testing against seven different classes of antibiotics was also performed on the same system using AST-N280 cards. The most dominant isolates identified were Acinetobacter baumannii, Morganella morganii, Kluyvera intermedia, Stenotrophomonas maltophilia, Escherichia coli, Aeromonas hydrophila/caviae, Klebsiella pneumoniae, Enterobacter cloacae and Citrobacter freundii. The isolates were observed to be significantly resistant against the antibiotics amoxicillin-clavulanic acid, cefuroxime, cefuroxime axetil and colistin. Two of the isolates, E. cloacae sp. dissolvens and S. maltophilia, were found to be positive for ESBL activity encoded by blaCTX-M gene. The possible intrusion of hospital wastewater in domestic sewage is also discussed. This study may help assess the risk of wastewater reuse by detecting dominant bacteria as a step towards the development of new microbiological standards. HIGHLIGHTS Stenotrophomonas maltophilia found to be most resistant and E. coli least resistant among the isolates.; Five isolates found to be resistant to the last resort antibiotic Colistin.; Molecular determinant of two extended spectrum beta lactamase producing isolates found to be blaCTX-M gene.; This report might aid in devising a suitable strategy for reducing risk to human health upon treated wastewater reuse.;
format article
author Sandeep Singh Shekhawat
Niha Mohan Kulshreshtha
Rinki Mishra
Sudipti Arora
Vivekanand Vivekanand
Akhilendra Bhushan Gupta
author_facet Sandeep Singh Shekhawat
Niha Mohan Kulshreshtha
Rinki Mishra
Sudipti Arora
Vivekanand Vivekanand
Akhilendra Bhushan Gupta
author_sort Sandeep Singh Shekhawat
title Antibiotic resistance in a predominantly occurring Gram-negative bacterial community from treated sewage to assess the need for going beyond coliform standards
title_short Antibiotic resistance in a predominantly occurring Gram-negative bacterial community from treated sewage to assess the need for going beyond coliform standards
title_full Antibiotic resistance in a predominantly occurring Gram-negative bacterial community from treated sewage to assess the need for going beyond coliform standards
title_fullStr Antibiotic resistance in a predominantly occurring Gram-negative bacterial community from treated sewage to assess the need for going beyond coliform standards
title_full_unstemmed Antibiotic resistance in a predominantly occurring Gram-negative bacterial community from treated sewage to assess the need for going beyond coliform standards
title_sort antibiotic resistance in a predominantly occurring gram-negative bacterial community from treated sewage to assess the need for going beyond coliform standards
publisher IWA Publishing
publishDate 2021
url https://doaj.org/article/a18d3aab12184c35adacd9481186c124
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