Evaluation of a panel of spermatological methods for assessing reprotoxic compounds in multilayer semen plastic bags

Abstract The increase of fertility performance in sows is one of the biggest achievements in pig production over the last 30 years. Nevertheless, pig farms using artificial insemination (AI) repeatedly experienced in recent year’s fertility problems with dramatic consequences due to toxic compounds...

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Autores principales:	M. Schulze, F. Schröter, M. Jung, U. Jakop
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a1917d7edeac44648372a21e3633ea5c
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spelling	oai:doaj.org-article:a1917d7edeac44648372a21e3633ea5c2021-12-02T13:58:10ZEvaluation of a panel of spermatological methods for assessing reprotoxic compounds in multilayer semen plastic bags10.1038/s41598-020-79415-72045-2322https://doaj.org/article/a1917d7edeac44648372a21e3633ea5c2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79415-7https://doaj.org/toc/2045-2322Abstract The increase of fertility performance in sows is one of the biggest achievements in pig production over the last 30 years. Nevertheless, pig farms using artificial insemination (AI) repeatedly experienced in recent year’s fertility problems with dramatic consequences due to toxic compounds from plastic semen bags. In particular, bisphenol A diglycidyl-ether (BADGE) present in multilayer plastic bags can leach into the semen and could affect the functionality of the spermatozoa. Former studies could not find any alterations in spermatozoa based on the exposure to BADGE. The aim of the study was to evaluate effects of BADGE on boar spermatozoa using an extended panel of spermatological methods. In spring 2019, a large drop in farrowing rates from 92.6 ± 2.3% to 63.7 ± 11.1% in four sow farms in Croatia was detected. In migration studies, BADGE could be identified as a causal toxic compound and leached into the extended semen in concentration of 0.37 ± 0.05 mg/L. Detailed spermatological studies showed that significant predictors for effects on spermatozoa were different levels of motility and kinematic data after a prolonged storage time, thermo-resistance test (prolonged incubation time), mitochondrial activity, membrane integrity and fluidity. No serious effects were observed for sperm morphology and DNA fragmentation. These results provide new insights into the development of a new quality assurance concept for a detailed spermatological examination during testing of plastic materials for boar semen preservation. It could be shown that boar spermatozoa are an excellent biosensor to detect potential toxicity and fertility-relevant compounds.M. SchulzeF. SchröterM. JungU. JakopNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. Schulze F. Schröter M. Jung U. Jakop Evaluation of a panel of spermatological methods for assessing reprotoxic compounds in multilayer semen plastic bags
description	Abstract The increase of fertility performance in sows is one of the biggest achievements in pig production over the last 30 years. Nevertheless, pig farms using artificial insemination (AI) repeatedly experienced in recent year’s fertility problems with dramatic consequences due to toxic compounds from plastic semen bags. In particular, bisphenol A diglycidyl-ether (BADGE) present in multilayer plastic bags can leach into the semen and could affect the functionality of the spermatozoa. Former studies could not find any alterations in spermatozoa based on the exposure to BADGE. The aim of the study was to evaluate effects of BADGE on boar spermatozoa using an extended panel of spermatological methods. In spring 2019, a large drop in farrowing rates from 92.6 ± 2.3% to 63.7 ± 11.1% in four sow farms in Croatia was detected. In migration studies, BADGE could be identified as a causal toxic compound and leached into the extended semen in concentration of 0.37 ± 0.05 mg/L. Detailed spermatological studies showed that significant predictors for effects on spermatozoa were different levels of motility and kinematic data after a prolonged storage time, thermo-resistance test (prolonged incubation time), mitochondrial activity, membrane integrity and fluidity. No serious effects were observed for sperm morphology and DNA fragmentation. These results provide new insights into the development of a new quality assurance concept for a detailed spermatological examination during testing of plastic materials for boar semen preservation. It could be shown that boar spermatozoa are an excellent biosensor to detect potential toxicity and fertility-relevant compounds.
format	article
author	M. Schulze F. Schröter M. Jung U. Jakop
author_facet	M. Schulze F. Schröter M. Jung U. Jakop
author_sort	M. Schulze
title	Evaluation of a panel of spermatological methods for assessing reprotoxic compounds in multilayer semen plastic bags
title_short	Evaluation of a panel of spermatological methods for assessing reprotoxic compounds in multilayer semen plastic bags
title_full	Evaluation of a panel of spermatological methods for assessing reprotoxic compounds in multilayer semen plastic bags
title_fullStr	Evaluation of a panel of spermatological methods for assessing reprotoxic compounds in multilayer semen plastic bags
title_full_unstemmed	Evaluation of a panel of spermatological methods for assessing reprotoxic compounds in multilayer semen plastic bags
title_sort	evaluation of a panel of spermatological methods for assessing reprotoxic compounds in multilayer semen plastic bags
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/a1917d7edeac44648372a21e3633ea5c
work_keys_str_mv	AT mschulze evaluationofapanelofspermatologicalmethodsforassessingreprotoxiccompoundsinmultilayersemenplasticbags AT fschroter evaluationofapanelofspermatologicalmethodsforassessingreprotoxiccompoundsinmultilayersemenplasticbags AT mjung evaluationofapanelofspermatologicalmethodsforassessingreprotoxiccompoundsinmultilayersemenplasticbags AT ujakop evaluationofapanelofspermatologicalmethodsforassessingreprotoxiccompoundsinmultilayersemenplasticbags
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Evaluation of a panel of spermatological methods for assessing reprotoxic compounds in multilayer semen plastic bags

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