Regulated and Non-Regulated Mycotoxin Detection in Cereal Matrices Using an Ultra-High-Performance Liquid Chromatography High-Resolution Mass Spectrometry (UHPLC-HRMS) Method

Cereals represent a widely consumed food commodity that might be contaminated by mycotoxins, resulting not only in potential consumer health risks upon dietary exposure but also significant financial losses due to contaminated batch disposal. Thus, continuous improvement of the performance character...

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Autores principales:	Aristeidis S. Tsagkaris, Nela Prusova, Zbynek Dzuman, Jana Pulkrabova, Jana Hajslova
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mycotoxins ergot alkaloids ultra-high-performance liquid chromatography high-resolution mass spectrometry cereal Medicine R
Acceso en línea:	https://doaj.org/article/a196e910930f447aae53cd0cff53aa2a
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spelling	oai:doaj.org-article:a196e910930f447aae53cd0cff53aa2a2021-11-25T19:08:48ZRegulated and Non-Regulated Mycotoxin Detection in Cereal Matrices Using an Ultra-High-Performance Liquid Chromatography High-Resolution Mass Spectrometry (UHPLC-HRMS) Method10.3390/toxins131107832072-6651https://doaj.org/article/a196e910930f447aae53cd0cff53aa2a2021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/783https://doaj.org/toc/2072-6651Cereals represent a widely consumed food commodity that might be contaminated by mycotoxins, resulting not only in potential consumer health risks upon dietary exposure but also significant financial losses due to contaminated batch disposal. Thus, continuous improvement of the performance characteristics of methods to enable an effective monitoring of such contaminants in food supply is highly needed. In this study, an ultra-high-performance liquid chromatography coupled to a hybrid quadrupole orbitrap mass analyzer (UHPLC-q-Orbitrap MS) method was optimized and validated in wheat, maize and rye flour matrices. Nineteen analytes were monitored, including both regulated mycotoxins, e.g., ochratoxin A (OTA) or deoxynivalenol (DON), and non-regulated mycotoxins, such as ergot alkaloids (EAs), which are analytes that are expected to be regulated soon in the EU. Low limits of quantification (LOQ) at the part per trillion level were achieved as well as wide linear ranges (four orders of magnitude) and recovery rates within the 68–104% range. Overall, the developed method attained fit-for-purpose results and it highlights the applicability of high-resolution mass spectrometry (HRMS) detection in mycotoxin food analysis.Aristeidis S. TsagkarisNela PrusovaZbynek DzumanJana PulkrabovaJana HajslovaMDPI AGarticlemycotoxinsergot alkaloidsultra-high-performance liquid chromatographyhigh-resolution mass spectrometrycerealMedicineRENToxins, Vol 13, Iss 783, p 783 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mycotoxins ergot alkaloids ultra-high-performance liquid chromatography high-resolution mass spectrometry cereal Medicine R
spellingShingle	mycotoxins ergot alkaloids ultra-high-performance liquid chromatography high-resolution mass spectrometry cereal Medicine R Aristeidis S. Tsagkaris Nela Prusova Zbynek Dzuman Jana Pulkrabova Jana Hajslova Regulated and Non-Regulated Mycotoxin Detection in Cereal Matrices Using an Ultra-High-Performance Liquid Chromatography High-Resolution Mass Spectrometry (UHPLC-HRMS) Method
description	Cereals represent a widely consumed food commodity that might be contaminated by mycotoxins, resulting not only in potential consumer health risks upon dietary exposure but also significant financial losses due to contaminated batch disposal. Thus, continuous improvement of the performance characteristics of methods to enable an effective monitoring of such contaminants in food supply is highly needed. In this study, an ultra-high-performance liquid chromatography coupled to a hybrid quadrupole orbitrap mass analyzer (UHPLC-q-Orbitrap MS) method was optimized and validated in wheat, maize and rye flour matrices. Nineteen analytes were monitored, including both regulated mycotoxins, e.g., ochratoxin A (OTA) or deoxynivalenol (DON), and non-regulated mycotoxins, such as ergot alkaloids (EAs), which are analytes that are expected to be regulated soon in the EU. Low limits of quantification (LOQ) at the part per trillion level were achieved as well as wide linear ranges (four orders of magnitude) and recovery rates within the 68–104% range. Overall, the developed method attained fit-for-purpose results and it highlights the applicability of high-resolution mass spectrometry (HRMS) detection in mycotoxin food analysis.
format	article
author	Aristeidis S. Tsagkaris Nela Prusova Zbynek Dzuman Jana Pulkrabova Jana Hajslova
author_facet	Aristeidis S. Tsagkaris Nela Prusova Zbynek Dzuman Jana Pulkrabova Jana Hajslova
author_sort	Aristeidis S. Tsagkaris
title	Regulated and Non-Regulated Mycotoxin Detection in Cereal Matrices Using an Ultra-High-Performance Liquid Chromatography High-Resolution Mass Spectrometry (UHPLC-HRMS) Method
title_short	Regulated and Non-Regulated Mycotoxin Detection in Cereal Matrices Using an Ultra-High-Performance Liquid Chromatography High-Resolution Mass Spectrometry (UHPLC-HRMS) Method
title_full	Regulated and Non-Regulated Mycotoxin Detection in Cereal Matrices Using an Ultra-High-Performance Liquid Chromatography High-Resolution Mass Spectrometry (UHPLC-HRMS) Method
title_fullStr	Regulated and Non-Regulated Mycotoxin Detection in Cereal Matrices Using an Ultra-High-Performance Liquid Chromatography High-Resolution Mass Spectrometry (UHPLC-HRMS) Method
title_full_unstemmed	Regulated and Non-Regulated Mycotoxin Detection in Cereal Matrices Using an Ultra-High-Performance Liquid Chromatography High-Resolution Mass Spectrometry (UHPLC-HRMS) Method
title_sort	regulated and non-regulated mycotoxin detection in cereal matrices using an ultra-high-performance liquid chromatography high-resolution mass spectrometry (uhplc-hrms) method
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a196e910930f447aae53cd0cff53aa2a
work_keys_str_mv	AT aristeidisstsagkaris regulatedandnonregulatedmycotoxindetectionincerealmatricesusinganultrahighperformanceliquidchromatographyhighresolutionmassspectrometryuhplchrmsmethod AT nelaprusova regulatedandnonregulatedmycotoxindetectionincerealmatricesusinganultrahighperformanceliquidchromatographyhighresolutionmassspectrometryuhplchrmsmethod AT zbynekdzuman regulatedandnonregulatedmycotoxindetectionincerealmatricesusinganultrahighperformanceliquidchromatographyhighresolutionmassspectrometryuhplchrmsmethod AT janapulkrabova regulatedandnonregulatedmycotoxindetectionincerealmatricesusinganultrahighperformanceliquidchromatographyhighresolutionmassspectrometryuhplchrmsmethod AT janahajslova regulatedandnonregulatedmycotoxindetectionincerealmatricesusinganultrahighperformanceliquidchromatographyhighresolutionmassspectrometryuhplchrmsmethod
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Regulated and Non-Regulated Mycotoxin Detection in Cereal Matrices Using an Ultra-High-Performance Liquid Chromatography High-Resolution Mass Spectrometry (UHPLC-HRMS) Method

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