Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid

Abstract Evaluate the biological action of valproic acid in the acetylation of histones and in the methylation of tumor suppressor genes via oral rinse in patients with a previous history of head and neck squamous cell carcinoma (HNSCC). Forty-two active or former smokers were included in this rando...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ricardo Ribeiro Gama, Lidia Maria Rebolho Batista Arantes, Bruna Pereira Sorroche, Pedro De Marchi, Matias Eliseo Melendez, Raiany Santos Carvalho, Marcos Alves de Lima, André Luiz Vettore, André Lopes Carvalho
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a1a750191e93469b91cc45c85575df0e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a1a750191e93469b91cc45c85575df0e
record_format	dspace
spelling	oai:doaj.org-article:a1a750191e93469b91cc45c85575df0e2021-12-02T15:07:47ZEvaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid10.1038/s41598-021-95845-32045-2322https://doaj.org/article/a1a750191e93469b91cc45c85575df0e2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95845-3https://doaj.org/toc/2045-2322Abstract Evaluate the biological action of valproic acid in the acetylation of histones and in the methylation of tumor suppressor genes via oral rinse in patients with a previous history of head and neck squamous cell carcinoma (HNSCC). Forty-two active or former smokers were included in this randomized, double-blind, placebo-controlled trial. Oral rinse samples were collected prior to treatment with valproic acid or placebo and after 90 days of treatment. The methylation status of five tumor suppressor genes and histone acetylation were evaluated by pyrosequencing and ELISA techniques, respectively. Differences between the 90-day and baseline oral rinse acetylation and methylation results were analyzed by comparing groups. Thirty-four patients were considered for analysis. The mean percentage adherence in the valproic and placebo groups was 93.4 and 93.0, respectively (p = 0.718). There was no statistically significant difference between groups when comparing the medians of the histone acetylation ratio and the methylation ratio for most of the studied genes. A significant reduction in the DCC methylation pattern was observed in the valproic group (p = 0.023). The use of valproic acid was safe and accompanied by good therapeutic adherence. DCC methylation was lower in the valproic acid group than in the placebo group.Ricardo Ribeiro GamaLidia Maria Rebolho Batista ArantesBruna Pereira SorrochePedro De MarchiMatias Eliseo MelendezRaiany Santos CarvalhoMarcos Alves de LimaAndré Luiz VettoreAndré Lopes CarvalhoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ricardo Ribeiro Gama Lidia Maria Rebolho Batista Arantes Bruna Pereira Sorroche Pedro De Marchi Matias Eliseo Melendez Raiany Santos Carvalho Marcos Alves de Lima André Luiz Vettore André Lopes Carvalho Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid
description	Abstract Evaluate the biological action of valproic acid in the acetylation of histones and in the methylation of tumor suppressor genes via oral rinse in patients with a previous history of head and neck squamous cell carcinoma (HNSCC). Forty-two active or former smokers were included in this randomized, double-blind, placebo-controlled trial. Oral rinse samples were collected prior to treatment with valproic acid or placebo and after 90 days of treatment. The methylation status of five tumor suppressor genes and histone acetylation were evaluated by pyrosequencing and ELISA techniques, respectively. Differences between the 90-day and baseline oral rinse acetylation and methylation results were analyzed by comparing groups. Thirty-four patients were considered for analysis. The mean percentage adherence in the valproic and placebo groups was 93.4 and 93.0, respectively (p = 0.718). There was no statistically significant difference between groups when comparing the medians of the histone acetylation ratio and the methylation ratio for most of the studied genes. A significant reduction in the DCC methylation pattern was observed in the valproic group (p = 0.023). The use of valproic acid was safe and accompanied by good therapeutic adherence. DCC methylation was lower in the valproic acid group than in the placebo group.
format	article
author	Ricardo Ribeiro Gama Lidia Maria Rebolho Batista Arantes Bruna Pereira Sorroche Pedro De Marchi Matias Eliseo Melendez Raiany Santos Carvalho Marcos Alves de Lima André Luiz Vettore André Lopes Carvalho
author_facet	Ricardo Ribeiro Gama Lidia Maria Rebolho Batista Arantes Bruna Pereira Sorroche Pedro De Marchi Matias Eliseo Melendez Raiany Santos Carvalho Marcos Alves de Lima André Luiz Vettore André Lopes Carvalho
author_sort	Ricardo Ribeiro Gama
title	Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid
title_short	Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid
title_full	Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid
title_fullStr	Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid
title_full_unstemmed	Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid
title_sort	evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a1a750191e93469b91cc45c85575df0e
work_keys_str_mv	AT ricardoribeirogama evaluationofacetylationandmethylationinoralrinseofpatientswithheadandneckcancerhistoryexposedtovalproicacid AT lidiamariarebolhobatistaarantes evaluationofacetylationandmethylationinoralrinseofpatientswithheadandneckcancerhistoryexposedtovalproicacid AT brunapereirasorroche evaluationofacetylationandmethylationinoralrinseofpatientswithheadandneckcancerhistoryexposedtovalproicacid AT pedrodemarchi evaluationofacetylationandmethylationinoralrinseofpatientswithheadandneckcancerhistoryexposedtovalproicacid AT matiaseliseomelendez evaluationofacetylationandmethylationinoralrinseofpatientswithheadandneckcancerhistoryexposedtovalproicacid AT raianysantoscarvalho evaluationofacetylationandmethylationinoralrinseofpatientswithheadandneckcancerhistoryexposedtovalproicacid AT marcosalvesdelima evaluationofacetylationandmethylationinoralrinseofpatientswithheadandneckcancerhistoryexposedtovalproicacid AT andreluizvettore evaluationofacetylationandmethylationinoralrinseofpatientswithheadandneckcancerhistoryexposedtovalproicacid AT andrelopescarvalho evaluationofacetylationandmethylationinoralrinseofpatientswithheadandneckcancerhistoryexposedtovalproicacid
_version_	1718388407775789056

Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid

Ejemplares similares