Xylella fastidiosa differentially accumulates mineral elements in biofilm and planktonic cells.

Xylella fastidiosa is a bacterial plant pathogen that infects numerous plant hosts. Disease develops when the bacterium colonizes the xylem vessels and forms a biofilm. Inductively coupled plasma optical emission spectroscopy was used to examine the mineral element content of this pathogen in biofil...

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Autores principales:	Paul A Cobine, Luisa F Cruz, Fernando Navarrete, Daniel Duncan, Melissa Tygart, Leonardo De La Fuente
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a1ac3008628042c586d83204002b92e1
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spelling	oai:doaj.org-article:a1ac3008628042c586d83204002b92e12021-11-18T08:00:32ZXylella fastidiosa differentially accumulates mineral elements in biofilm and planktonic cells.1932-620310.1371/journal.pone.0054936https://doaj.org/article/a1ac3008628042c586d83204002b92e12013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23349991/?tool=EBIhttps://doaj.org/toc/1932-6203Xylella fastidiosa is a bacterial plant pathogen that infects numerous plant hosts. Disease develops when the bacterium colonizes the xylem vessels and forms a biofilm. Inductively coupled plasma optical emission spectroscopy was used to examine the mineral element content of this pathogen in biofilm and planktonic states. Significant accumulations of copper (30-fold), manganese (6-fold), zinc (5-fold), calcium (2-fold) and potassium (2-fold) in the biofilm compared to planktonic cells were observed. Other mineral elements such as sodium, magnesium and iron did not significantly differ between biofilm and planktonic cells. The distribution of mineral elements in the planktonic cells loosely mirrors the media composition; however the unique mineral element distribution in biofilm suggests specific mechanisms of accumulation from the media. A cell-to-surface attachment assay shows that addition of 50 to 100 µM Cu to standard X. fastidiosa media increases biofilm, while higher concentrations (>200 µM) slow cell growth and prevent biofilm formation. Moreover cell-to-surface attachment was blocked by specific chelation of copper. Growth of X. fastidiosa in microfluidic chambers under flow conditions showed that addition of 50 µM Cu to the media accelerated attachment and aggregation, while 400 µM prevented this process. Supplementation of standard media with Mn showed increased biofilm formation and cell-to-cell attachment. In contrast, while the biofilm accumulated Zn, supplementation to the media with this element caused inhibited growth of planktonic cells and impaired biofilm formation. Collectively these data suggest roles for these minerals in attachment and biofilm formation and therefore the virulence of this pathogen.Paul A CobineLuisa F CruzFernando NavarreteDaniel DuncanMelissa TygartLeonardo De La FuentePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e54936 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paul A Cobine Luisa F Cruz Fernando Navarrete Daniel Duncan Melissa Tygart Leonardo De La Fuente Xylella fastidiosa differentially accumulates mineral elements in biofilm and planktonic cells.
description	Xylella fastidiosa is a bacterial plant pathogen that infects numerous plant hosts. Disease develops when the bacterium colonizes the xylem vessels and forms a biofilm. Inductively coupled plasma optical emission spectroscopy was used to examine the mineral element content of this pathogen in biofilm and planktonic states. Significant accumulations of copper (30-fold), manganese (6-fold), zinc (5-fold), calcium (2-fold) and potassium (2-fold) in the biofilm compared to planktonic cells were observed. Other mineral elements such as sodium, magnesium and iron did not significantly differ between biofilm and planktonic cells. The distribution of mineral elements in the planktonic cells loosely mirrors the media composition; however the unique mineral element distribution in biofilm suggests specific mechanisms of accumulation from the media. A cell-to-surface attachment assay shows that addition of 50 to 100 µM Cu to standard X. fastidiosa media increases biofilm, while higher concentrations (>200 µM) slow cell growth and prevent biofilm formation. Moreover cell-to-surface attachment was blocked by specific chelation of copper. Growth of X. fastidiosa in microfluidic chambers under flow conditions showed that addition of 50 µM Cu to the media accelerated attachment and aggregation, while 400 µM prevented this process. Supplementation of standard media with Mn showed increased biofilm formation and cell-to-cell attachment. In contrast, while the biofilm accumulated Zn, supplementation to the media with this element caused inhibited growth of planktonic cells and impaired biofilm formation. Collectively these data suggest roles for these minerals in attachment and biofilm formation and therefore the virulence of this pathogen.
format	article
author	Paul A Cobine Luisa F Cruz Fernando Navarrete Daniel Duncan Melissa Tygart Leonardo De La Fuente
author_facet	Paul A Cobine Luisa F Cruz Fernando Navarrete Daniel Duncan Melissa Tygart Leonardo De La Fuente
author_sort	Paul A Cobine
title	Xylella fastidiosa differentially accumulates mineral elements in biofilm and planktonic cells.
title_short	Xylella fastidiosa differentially accumulates mineral elements in biofilm and planktonic cells.
title_full	Xylella fastidiosa differentially accumulates mineral elements in biofilm and planktonic cells.
title_fullStr	Xylella fastidiosa differentially accumulates mineral elements in biofilm and planktonic cells.
title_full_unstemmed	Xylella fastidiosa differentially accumulates mineral elements in biofilm and planktonic cells.
title_sort	xylella fastidiosa differentially accumulates mineral elements in biofilm and planktonic cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a1ac3008628042c586d83204002b92e1
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Xylella fastidiosa differentially accumulates mineral elements in biofilm and planktonic cells.

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