Identification of miRNAs differentially expressed in human epilepsy with or without granule cell pathology.

The microRNAs (miRNAs) are small size non-coding RNAs that regulate expression of target mRNAs at post-transcriptional level. miRNAs differentially expressed under pathological conditions may help identifying mechanisms underlying the disease and may represent biomarkers with prognostic value. Howev...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Silvia Zucchini, Gianluca Marucci, Beatrice Paradiso, Giovanni Lanza, Paolo Roncon, Pierangelo Cifelli, Manuela Ferracin, Marco Giulioni, Roberto Michelucci, Guido Rubboli, Michele Simonato
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a1b8086a0c51450186534d9f2298521f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a1b8086a0c51450186534d9f2298521f
record_format	dspace
spelling	oai:doaj.org-article:a1b8086a0c51450186534d9f2298521f2021-11-25T06:03:34ZIdentification of miRNAs differentially expressed in human epilepsy with or without granule cell pathology.1932-620310.1371/journal.pone.0105521https://doaj.org/article/a1b8086a0c51450186534d9f2298521f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25148080/?tool=EBIhttps://doaj.org/toc/1932-6203The microRNAs (miRNAs) are small size non-coding RNAs that regulate expression of target mRNAs at post-transcriptional level. miRNAs differentially expressed under pathological conditions may help identifying mechanisms underlying the disease and may represent biomarkers with prognostic value. However, this kind of studies are difficult in the brain because of the cellular heterogeneity of the tissue and of the limited access to fresh tissue. Here, we focused on a pathology affecting specific cells in a subpopulation of epileptic brains (hippocampal granule cells), an approach that bypasses the above problems. All patients underwent surgery for intractable temporal lobe epilepsy and had hippocampal sclerosis associated with no granule cell pathology in half of the cases and with type-2 granule cell pathology (granule cell layer dispersion or bilamination) in the other half. The expression of more than 1000 miRNAs was examined in the laser-microdissected dentate granule cell layer. Twelve miRNAs were differentially expressed in the two groups. One of these, miR487a, was confirmed to be expressed at highly differential levels in an extended cohort of patients, using RT-qPCR. Bioinformatics searches and RT-qPCR verification identified ANTXR1 as a possible target of miR487a. ANTXR1 may be directly implicated in granule cell dispersion because it is an adhesion molecule that favors cell spreading. Thus, miR487a could be the first identified element of a miRNA signature that may be useful for prognostic evaluation of post-surgical epilepsy and may drive mechanistic studies leading to the identification of therapeutic targets.Silvia ZucchiniGianluca MarucciBeatrice ParadisoGiovanni LanzaPaolo RonconPierangelo CifelliManuela FerracinMarco GiulioniRoberto MichelucciGuido RubboliMichele SimonatoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e105521 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Silvia Zucchini Gianluca Marucci Beatrice Paradiso Giovanni Lanza Paolo Roncon Pierangelo Cifelli Manuela Ferracin Marco Giulioni Roberto Michelucci Guido Rubboli Michele Simonato Identification of miRNAs differentially expressed in human epilepsy with or without granule cell pathology.
description	The microRNAs (miRNAs) are small size non-coding RNAs that regulate expression of target mRNAs at post-transcriptional level. miRNAs differentially expressed under pathological conditions may help identifying mechanisms underlying the disease and may represent biomarkers with prognostic value. However, this kind of studies are difficult in the brain because of the cellular heterogeneity of the tissue and of the limited access to fresh tissue. Here, we focused on a pathology affecting specific cells in a subpopulation of epileptic brains (hippocampal granule cells), an approach that bypasses the above problems. All patients underwent surgery for intractable temporal lobe epilepsy and had hippocampal sclerosis associated with no granule cell pathology in half of the cases and with type-2 granule cell pathology (granule cell layer dispersion or bilamination) in the other half. The expression of more than 1000 miRNAs was examined in the laser-microdissected dentate granule cell layer. Twelve miRNAs were differentially expressed in the two groups. One of these, miR487a, was confirmed to be expressed at highly differential levels in an extended cohort of patients, using RT-qPCR. Bioinformatics searches and RT-qPCR verification identified ANTXR1 as a possible target of miR487a. ANTXR1 may be directly implicated in granule cell dispersion because it is an adhesion molecule that favors cell spreading. Thus, miR487a could be the first identified element of a miRNA signature that may be useful for prognostic evaluation of post-surgical epilepsy and may drive mechanistic studies leading to the identification of therapeutic targets.
format	article
author	Silvia Zucchini Gianluca Marucci Beatrice Paradiso Giovanni Lanza Paolo Roncon Pierangelo Cifelli Manuela Ferracin Marco Giulioni Roberto Michelucci Guido Rubboli Michele Simonato
author_facet	Silvia Zucchini Gianluca Marucci Beatrice Paradiso Giovanni Lanza Paolo Roncon Pierangelo Cifelli Manuela Ferracin Marco Giulioni Roberto Michelucci Guido Rubboli Michele Simonato
author_sort	Silvia Zucchini
title	Identification of miRNAs differentially expressed in human epilepsy with or without granule cell pathology.
title_short	Identification of miRNAs differentially expressed in human epilepsy with or without granule cell pathology.
title_full	Identification of miRNAs differentially expressed in human epilepsy with or without granule cell pathology.
title_fullStr	Identification of miRNAs differentially expressed in human epilepsy with or without granule cell pathology.
title_full_unstemmed	Identification of miRNAs differentially expressed in human epilepsy with or without granule cell pathology.
title_sort	identification of mirnas differentially expressed in human epilepsy with or without granule cell pathology.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/a1b8086a0c51450186534d9f2298521f
work_keys_str_mv	AT silviazucchini identificationofmirnasdifferentiallyexpressedinhumanepilepsywithorwithoutgranulecellpathology AT gianlucamarucci identificationofmirnasdifferentiallyexpressedinhumanepilepsywithorwithoutgranulecellpathology AT beatriceparadiso identificationofmirnasdifferentiallyexpressedinhumanepilepsywithorwithoutgranulecellpathology AT giovannilanza identificationofmirnasdifferentiallyexpressedinhumanepilepsywithorwithoutgranulecellpathology AT paoloroncon identificationofmirnasdifferentiallyexpressedinhumanepilepsywithorwithoutgranulecellpathology AT pierangelocifelli identificationofmirnasdifferentiallyexpressedinhumanepilepsywithorwithoutgranulecellpathology AT manuelaferracin identificationofmirnasdifferentiallyexpressedinhumanepilepsywithorwithoutgranulecellpathology AT marcogiulioni identificationofmirnasdifferentiallyexpressedinhumanepilepsywithorwithoutgranulecellpathology AT robertomichelucci identificationofmirnasdifferentiallyexpressedinhumanepilepsywithorwithoutgranulecellpathology AT guidorubboli identificationofmirnasdifferentiallyexpressedinhumanepilepsywithorwithoutgranulecellpathology AT michelesimonato identificationofmirnasdifferentiallyexpressedinhumanepilepsywithorwithoutgranulecellpathology
_version_	1718414236949938176

Identification of miRNAs differentially expressed in human epilepsy with or without granule cell pathology.

Ejemplares similares