Rapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature
Abstract Despite ongoing efforts to characterize the host response toward SARS-CoV-2, a major gap in our knowledge still exists regarding the magnitude and duration of the humoral response. Analysis of the antibody response in mild versus moderate/severe patients, using our new developed quantitativ...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/a1ba2337325c40c4bd8595bff203b6bf |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:a1ba2337325c40c4bd8595bff203b6bf |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:a1ba2337325c40c4bd8595bff203b6bf2021-12-02T12:09:32ZRapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature10.1038/s41598-021-83019-02045-2322https://doaj.org/article/a1ba2337325c40c4bd8595bff203b6bf2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83019-0https://doaj.org/toc/2045-2322Abstract Despite ongoing efforts to characterize the host response toward SARS-CoV-2, a major gap in our knowledge still exists regarding the magnitude and duration of the humoral response. Analysis of the antibody response in mild versus moderate/severe patients, using our new developed quantitative electrochemiluminescent assay for detecting IgM/IgA/IgG antibodies toward SARS-CoV-2 antigens, revealed a rapid onset of IgG/IgA antibodies, specifically in moderate/severe patients. IgM antibodies against the viral receptor binding domain, but not against nucleocapsid protein, were detected at early stages of the disease. Furthermore, we observed a marked reduction in IgM/IgA antibodies over-time. Adapting our assay for ACE2 binding-competition, demonstrated that the presence of potentially neutralizing antibodies is corelated with IgG/IgA. Finally, analysis of the cytokine profile in COVID-19 patients revealed unique correlation of an IL-12p70/IL33 and IgG seroconversion, which correlated with disease severity. In summary, our comprehensive analysis has major implications on the understanding and monitoring of SARS-CoV-2 infections.Ariel MunitzL. Edry-BotzerM. ItanR. Tur-KaspaD. DickerD. MarcoviciuM. G. GorenM. MorS. LevT. GottesmanK. MuhsenD. CohenM. SteinU. QimronN. T. FreundY. WineMotti GerlicNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Ariel Munitz L. Edry-Botzer M. Itan R. Tur-Kaspa D. Dicker D. Marcoviciu M. G. Goren M. Mor S. Lev T. Gottesman K. Muhsen D. Cohen M. Stein U. Qimron N. T. Freund Y. Wine Motti Gerlic Rapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature |
| description |
Abstract Despite ongoing efforts to characterize the host response toward SARS-CoV-2, a major gap in our knowledge still exists regarding the magnitude and duration of the humoral response. Analysis of the antibody response in mild versus moderate/severe patients, using our new developed quantitative electrochemiluminescent assay for detecting IgM/IgA/IgG antibodies toward SARS-CoV-2 antigens, revealed a rapid onset of IgG/IgA antibodies, specifically in moderate/severe patients. IgM antibodies against the viral receptor binding domain, but not against nucleocapsid protein, were detected at early stages of the disease. Furthermore, we observed a marked reduction in IgM/IgA antibodies over-time. Adapting our assay for ACE2 binding-competition, demonstrated that the presence of potentially neutralizing antibodies is corelated with IgG/IgA. Finally, analysis of the cytokine profile in COVID-19 patients revealed unique correlation of an IL-12p70/IL33 and IgG seroconversion, which correlated with disease severity. In summary, our comprehensive analysis has major implications on the understanding and monitoring of SARS-CoV-2 infections. |
| format |
article |
| author |
Ariel Munitz L. Edry-Botzer M. Itan R. Tur-Kaspa D. Dicker D. Marcoviciu M. G. Goren M. Mor S. Lev T. Gottesman K. Muhsen D. Cohen M. Stein U. Qimron N. T. Freund Y. Wine Motti Gerlic |
| author_facet |
Ariel Munitz L. Edry-Botzer M. Itan R. Tur-Kaspa D. Dicker D. Marcoviciu M. G. Goren M. Mor S. Lev T. Gottesman K. Muhsen D. Cohen M. Stein U. Qimron N. T. Freund Y. Wine Motti Gerlic |
| author_sort |
Ariel Munitz |
| title |
Rapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature |
| title_short |
Rapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature |
| title_full |
Rapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature |
| title_fullStr |
Rapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature |
| title_full_unstemmed |
Rapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature |
| title_sort |
rapid seroconversion and persistent functional igg antibodies in severe covid-19 patients correlates with an il-12p70 and il-33 signature |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/a1ba2337325c40c4bd8595bff203b6bf |
| work_keys_str_mv |
AT arielmunitz rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT ledrybotzer rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT mitan rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT rturkaspa rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT ddicker rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT dmarcoviciu rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT mggoren rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT mmor rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT slev rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT tgottesman rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT kmuhsen rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT dcohen rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT mstein rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT uqimron rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT ntfreund rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT ywine rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature AT mottigerlic rapidseroconversionandpersistentfunctionaliggantibodiesinseverecovid19patientscorrelateswithanil12p70andil33signature |
| _version_ |
1718394646229417984 |