Detection of ROS1 rearrangement in non-small cell lung cancer: current and future perspectives

Giulio Rossi,1 Genny Jocollé,2 Antonia Conti,3 Marcello Tiseo,4 Federica Zito Marino,5,6 Giovanni Donati,7 Renato Franco,5,6 Francesca Bono,8 Francesca Barbisan,9 Francesco Facchinetti4,10 1Pathology Unit, 2Oncology Unit, Azienda USL Valle d’Aosta, Regional Hospital “P...

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Autores principales:	Rossi G, Jocollé G, Conti A, Tiseo M, Zito Marino F, Donati G, Franco R, Bono F, Barbisan F, Facchinetti F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	lung adenocarcinoma ROS1 FISH immunohistochemistry NGS rearrangement Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/a1ba6c03f55d443a8bc0cfe41a8f2e7e
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spelling	oai:doaj.org-article:a1ba6c03f55d443a8bc0cfe41a8f2e7e2021-12-02T08:52:04ZDetection of ROS1 rearrangement in non-small cell lung cancer: current and future perspectives1179-2728https://doaj.org/article/a1ba6c03f55d443a8bc0cfe41a8f2e7e2017-07-01T00:00:00Zhttps://www.dovepress.com/detection-of-ros1-rearrangement-in-non-small-cell-lung-cancer-current--peer-reviewed-article-LCTThttps://doaj.org/toc/1179-2728Giulio Rossi,1 Genny Jocollé,2 Antonia Conti,3 Marcello Tiseo,4 Federica Zito Marino,5,6 Giovanni Donati,7 Renato Franco,5,6 Francesca Bono,8 Francesca Barbisan,9 Francesco Facchinetti4,10 1Pathology Unit, 2Oncology Unit, Azienda USL Valle d’Aosta, Regional Hospital “Parini”, Aosta, 3Medical Illustrator, Riccione, 4Medical Oncology Unit, University Hospital of Parma, Parma, 5Pathology Unit, Istituto Nazionale Tumori Fondazione G. Pascale, 6Pathology Unit, Luigi Vanvitelli University of Campania, Naples, 7Unit of Thoracic and Senology Surgery, Azienda USL Valle d’Aosta, Regional Hospital “Parini”, Aosta, 8Unit of Pathologic Anatomy, San Gerardo Hospital, IRCCS, Monza, 9Pathology Unit, University Hospital, Azienda Ospedali Riuniti, Ancona, Italy; 10INSERM, U981, Gustave Roussy Cancer Campus, Villejuif, France Abstract: ROS1 rearrangement characterizes a small subset (1%–2%) of non-small cell lung cancer and is associated with slight/never smoking patients and adenocarcinoma histology. Identification of ROS1 rearrangement is mandatory to permit targeted therapy with specific inhibitors, demonstrating a significantly better survival when compared with conventional chemotherapy. Detection of ROS1 rearrangement is based on in situ (immunohistochemistry, fluorescence in situ hybridization) and extractive non-in situ assays. While fluorescence in situ hybridization still represents the gold standard in clinical trials, this technique may fail to recognize rearrangements of ROS1 with some gene fusion partner. On the other hand, immunohistochemistry is the most cost-effective screening technique, but it seems to be characterized by low specificity. Extractive molecular assays are expensive and laborious methods, but they specifically recognize almost all ROS1 fusions using a limited amount of mRNA even from formalin-fixed, paraffin-embedded tumor tissues. This review is a discussion on the present and futuristic diagnostic scenario of ROS1 identification in lung cancer. Keywords: lung, adenocarcinoma, ROS1, FISH, immunohistochemistry, NGS, rearrangementRossi GJocollé GConti ATiseo MZito Marino FDonati GFranco RBono FBarbisan FFacchinetti FDove Medical PressarticlelungadenocarcinomaROS1FISHimmunohistochemistryNGSrearrangementNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENLung Cancer: Targets and Therapy, Vol Volume 8, Pp 45-55 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	lung adenocarcinoma ROS1 FISH immunohistochemistry NGS rearrangement Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	lung adenocarcinoma ROS1 FISH immunohistochemistry NGS rearrangement Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Rossi G Jocollé G Conti A Tiseo M Zito Marino F Donati G Franco R Bono F Barbisan F Facchinetti F Detection of ROS1 rearrangement in non-small cell lung cancer: current and future perspectives
description	Giulio Rossi,1 Genny Jocollé,2 Antonia Conti,3 Marcello Tiseo,4 Federica Zito Marino,5,6 Giovanni Donati,7 Renato Franco,5,6 Francesca Bono,8 Francesca Barbisan,9 Francesco Facchinetti4,10 1Pathology Unit, 2Oncology Unit, Azienda USL Valle d’Aosta, Regional Hospital “Parini”, Aosta, 3Medical Illustrator, Riccione, 4Medical Oncology Unit, University Hospital of Parma, Parma, 5Pathology Unit, Istituto Nazionale Tumori Fondazione G. Pascale, 6Pathology Unit, Luigi Vanvitelli University of Campania, Naples, 7Unit of Thoracic and Senology Surgery, Azienda USL Valle d’Aosta, Regional Hospital “Parini”, Aosta, 8Unit of Pathologic Anatomy, San Gerardo Hospital, IRCCS, Monza, 9Pathology Unit, University Hospital, Azienda Ospedali Riuniti, Ancona, Italy; 10INSERM, U981, Gustave Roussy Cancer Campus, Villejuif, France Abstract: ROS1 rearrangement characterizes a small subset (1%–2%) of non-small cell lung cancer and is associated with slight/never smoking patients and adenocarcinoma histology. Identification of ROS1 rearrangement is mandatory to permit targeted therapy with specific inhibitors, demonstrating a significantly better survival when compared with conventional chemotherapy. Detection of ROS1 rearrangement is based on in situ (immunohistochemistry, fluorescence in situ hybridization) and extractive non-in situ assays. While fluorescence in situ hybridization still represents the gold standard in clinical trials, this technique may fail to recognize rearrangements of ROS1 with some gene fusion partner. On the other hand, immunohistochemistry is the most cost-effective screening technique, but it seems to be characterized by low specificity. Extractive molecular assays are expensive and laborious methods, but they specifically recognize almost all ROS1 fusions using a limited amount of mRNA even from formalin-fixed, paraffin-embedded tumor tissues. This review is a discussion on the present and futuristic diagnostic scenario of ROS1 identification in lung cancer. Keywords: lung, adenocarcinoma, ROS1, FISH, immunohistochemistry, NGS, rearrangement
format	article
author	Rossi G Jocollé G Conti A Tiseo M Zito Marino F Donati G Franco R Bono F Barbisan F Facchinetti F
author_facet	Rossi G Jocollé G Conti A Tiseo M Zito Marino F Donati G Franco R Bono F Barbisan F Facchinetti F
author_sort	Rossi G
title	Detection of ROS1 rearrangement in non-small cell lung cancer: current and future perspectives
title_short	Detection of ROS1 rearrangement in non-small cell lung cancer: current and future perspectives
title_full	Detection of ROS1 rearrangement in non-small cell lung cancer: current and future perspectives
title_fullStr	Detection of ROS1 rearrangement in non-small cell lung cancer: current and future perspectives
title_full_unstemmed	Detection of ROS1 rearrangement in non-small cell lung cancer: current and future perspectives
title_sort	detection of ros1 rearrangement in non-small cell lung cancer: current and future perspectives
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/a1ba6c03f55d443a8bc0cfe41a8f2e7e
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Detection of ROS1 rearrangement in non-small cell lung cancer: current and future perspectives

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