Amelioration effects of nanoencapsulated triterpenoids from petri dish-cultured Antrodia cinnamomea on reproductive function of diabetic male rats

Sabri Sudirman, Yuan-Hua Hsu, Athira Johnson, David Tsou, Zwe-Ling Kong Department of Food Science, National Taiwan Ocean University, Keelung City, Taiwan, Republic of China Purpose: Nanoencapsulated triterpenoids from petri dish-cultured Antrodia cinnamomea (PAC) and its amelioration effects on r...

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Autores principales:	Sudirman S, Hsu YH, Johnson A, Tsou D, Kong ZL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Antrodia cinnamomea diabetes mellitus male reproduction nanoparticles Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a1cb3989b39944cdbdfdaaddb739efa9
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spelling	oai:doaj.org-article:a1cb3989b39944cdbdfdaaddb739efa92021-12-02T08:34:29ZAmelioration effects of nanoencapsulated triterpenoids from petri dish-cultured Antrodia cinnamomea on reproductive function of diabetic male rats1178-2013https://doaj.org/article/a1cb3989b39944cdbdfdaaddb739efa92018-09-01T00:00:00Zhttps://www.dovepress.com/amelioration-effects-of-nanoencapsulated-triterpenoids-from-petri-dish-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sabri Sudirman, Yuan-Hua Hsu, Athira Johnson, David Tsou, Zwe-Ling Kong Department of Food Science, National Taiwan Ocean University, Keelung City, Taiwan, Republic of China Purpose: Nanoencapsulated triterpenoids from petri dish-cultured Antrodia cinnamomea (PAC) and its amelioration effects on reproductive function in diabetic rats were investigated. Materials and methods: PAC encapsulated in silica–chitosan nanoparticles (Nano-PAC) was prepared by the biosilicification method. The diabetic condition in male Sprague Dawley rats was induced by high-fat diet and streptozotocin (STZ). Three different doses of Nano-PAC (4, 8, and 20 mg/kg) were administered for 6 weeks. Metformin and control of nanoparticles (Nano-con) were taken as positive and negative controls, respectively. Results: The average particle size was ~79.46±1.63 nm, and encapsulation efficiency was ~73.35%±0.09%. Nano-PAC administration improved hyperglycemia and insulin resistance. In addition, Nano-PAC ameliorated the morphology of testicular seminiferous tubules, sperm morphology, motility, ROS production, and mitochondrial membrane potential. Superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) antioxidant, as well as testosterone, luteinizing hormone (LH), and follicle stimulating hormone (FSH) were increased, whereas proinflammatory cytokines TNF-α, IL-6, and IFN-γ were decreased. Conclusion: In the present study, we successfully nanoencapsulated PAC and found that a very low dosage of Nano-PAC exhibited amelioration effects on the reproductive function of diabetic rats. Keywords: Antrodia cinnamomea, diabetes mellitus, insulin resistance, male reproduction, nanoparticlesSudirman SHsu YHJohnson ATsou DKong ZLDove Medical PressarticleAntrodia cinnamomeadiabetes mellitusmale reproductionnanoparticlesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 5059-5073 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Antrodia cinnamomea diabetes mellitus male reproduction nanoparticles Medicine (General) R5-920
spellingShingle	Antrodia cinnamomea diabetes mellitus male reproduction nanoparticles Medicine (General) R5-920 Sudirman S Hsu YH Johnson A Tsou D Kong ZL Amelioration effects of nanoencapsulated triterpenoids from petri dish-cultured Antrodia cinnamomea on reproductive function of diabetic male rats
description	Sabri Sudirman, Yuan-Hua Hsu, Athira Johnson, David Tsou, Zwe-Ling Kong Department of Food Science, National Taiwan Ocean University, Keelung City, Taiwan, Republic of China Purpose: Nanoencapsulated triterpenoids from petri dish-cultured Antrodia cinnamomea (PAC) and its amelioration effects on reproductive function in diabetic rats were investigated. Materials and methods: PAC encapsulated in silica–chitosan nanoparticles (Nano-PAC) was prepared by the biosilicification method. The diabetic condition in male Sprague Dawley rats was induced by high-fat diet and streptozotocin (STZ). Three different doses of Nano-PAC (4, 8, and 20 mg/kg) were administered for 6 weeks. Metformin and control of nanoparticles (Nano-con) were taken as positive and negative controls, respectively. Results: The average particle size was ~79.46±1.63 nm, and encapsulation efficiency was ~73.35%±0.09%. Nano-PAC administration improved hyperglycemia and insulin resistance. In addition, Nano-PAC ameliorated the morphology of testicular seminiferous tubules, sperm morphology, motility, ROS production, and mitochondrial membrane potential. Superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) antioxidant, as well as testosterone, luteinizing hormone (LH), and follicle stimulating hormone (FSH) were increased, whereas proinflammatory cytokines TNF-α, IL-6, and IFN-γ were decreased. Conclusion: In the present study, we successfully nanoencapsulated PAC and found that a very low dosage of Nano-PAC exhibited amelioration effects on the reproductive function of diabetic rats. Keywords: Antrodia cinnamomea, diabetes mellitus, insulin resistance, male reproduction, nanoparticles
format	article
author	Sudirman S Hsu YH Johnson A Tsou D Kong ZL
author_facet	Sudirman S Hsu YH Johnson A Tsou D Kong ZL
author_sort	Sudirman S
title	Amelioration effects of nanoencapsulated triterpenoids from petri dish-cultured Antrodia cinnamomea on reproductive function of diabetic male rats
title_short	Amelioration effects of nanoencapsulated triterpenoids from petri dish-cultured Antrodia cinnamomea on reproductive function of diabetic male rats
title_full	Amelioration effects of nanoencapsulated triterpenoids from petri dish-cultured Antrodia cinnamomea on reproductive function of diabetic male rats
title_fullStr	Amelioration effects of nanoencapsulated triterpenoids from petri dish-cultured Antrodia cinnamomea on reproductive function of diabetic male rats
title_full_unstemmed	Amelioration effects of nanoencapsulated triterpenoids from petri dish-cultured Antrodia cinnamomea on reproductive function of diabetic male rats
title_sort	amelioration effects of nanoencapsulated triterpenoids from petri dish-cultured antrodia cinnamomea on reproductive function of diabetic male rats
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/a1cb3989b39944cdbdfdaaddb739efa9
work_keys_str_mv	AT sudirmans ameliorationeffectsofnanoencapsulatedtriterpenoidsfrompetridishculturedantrodiacinnamomeaonreproductivefunctionofdiabeticmalerats AT hsuyh ameliorationeffectsofnanoencapsulatedtriterpenoidsfrompetridishculturedantrodiacinnamomeaonreproductivefunctionofdiabeticmalerats AT johnsona ameliorationeffectsofnanoencapsulatedtriterpenoidsfrompetridishculturedantrodiacinnamomeaonreproductivefunctionofdiabeticmalerats AT tsoud ameliorationeffectsofnanoencapsulatedtriterpenoidsfrompetridishculturedantrodiacinnamomeaonreproductivefunctionofdiabeticmalerats AT kongzl ameliorationeffectsofnanoencapsulatedtriterpenoidsfrompetridishculturedantrodiacinnamomeaonreproductivefunctionofdiabeticmalerats
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Amelioration effects of nanoencapsulated triterpenoids from petri dish-cultured Antrodia cinnamomea on reproductive function of diabetic male rats

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